Logo of jcellbiolHomeThe Rockefeller University PressEditorsContactInstructions for AuthorsThis issue
J Cell Biol. Mar 1, 1977; 72(3): 595–603.
PMCID: PMC2111017

Daughter 3T3 cells. Are they mirror images of each other?

Abstract

Using a new technique to visualize the tracks of moving 3T3 cells and combining it with the visualization of actin-containing microfilament bundles by indirect immunofluorescence (Lazarides, E. and K. Weber. 1974, Proc. Natl. Acad. Sci. U.S.A. 71:2268-2272), I present experiments which suggest that: (a) 30-40% of the pairs of daughter 3T3 mouse fibroblasts in noncloned cultures have mirror symmetrical actin-bundle patterns. (b) The angle between separating daughter cells is approx. 90 degrees or 180 degrees and seems related to the directions of certain actin-containing bundles. (c) Approximately 40% of separately moving daughter cells which did not collide with any other cell in the culture performed directional changes in a mirror symmetrical way. Both daughter cells entered the next mitosis at approximately the same time. I suggest that the actin-bundle pattern, the angle of separation, major directional changes during interphase, and the time of the next mitosis are predetermined by the parental cell.

Full Text

The Full Text of this article is available as a PDF (2.7M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • ABERCROMBIE M, HEAYSMAN JE. Observations on the social behaviour of cells in tissue culture. II. Monolayering of fibroblasts. Exp Cell Res. 1954 May;6(2):293–306. [PubMed]
  • Albrecht-Buehler G. Filopodia of spreading 3T3 cells. Do they have a substrate-exploring function? J Cell Biol. 1976 May;69(2):275–286. [PMC free article] [PubMed]
  • Albrecht-Buehler G, Goldman RD. Microspike-mediated particle transport towards the cell body during early spreading of 3T3 cells. Exp Cell Res. 1976 Feb;97(2):329–339. [PubMed]
  • Albrecht-Buehler G, Lancaster RM. A quantitative description of the extension and retraction of surface protrusions in spreading 3T3 mouse fibroblasts. J Cell Biol. 1976 Nov;71(2):370–382. [PMC free article] [PubMed]
  • Albrecht-Bühler G, Yarnell MM. A quantitation of movement of marker particles in the plasma membrane of 3T3 mouse fibroblasts. Experimental methods. Exp Cell Res. 1973 Mar 30;78(1):59–66. [PubMed]
  • Gail MH, Boone CW. The locomotion of mouse fibroblasts in tissue culture. Biophys J. 1970 Oct;10(10):980–993. [PMC free article] [PubMed]
  • Lazarides E, Weber K. Actin antibody: the specific visualization of actin filaments in non-muscle cells. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2268–2272. [PMC free article] [PubMed]
  • TODARO GJ, GREEN H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J Cell Biol. 1963 May;17:299–313. [PMC free article] [PubMed]
  • Trinkaus JP, Betchaku T, Krulikowski LS. Local inhibition of ruffling during contact inhibition of cell movement. Exp Cell Res. 1971 Feb;64(2):291–300. [PubMed]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...