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J Cell Biol. Nov 1, 1980; 87(2): 442–450.
PMCID: PMC2110758

Calcofluor white and Congo red inhibit chitin microfibril assembly of Poterioochromonas: evidence for a gap between polymerization and microfibril formation

Abstract

The influence of the light microscopical stains, Calcofluor white and Congo red, on the process of chitin microfibril formation of the chrysoflagellate alga Poterioochromonas stipitata was studied with light and electron microscopy. There is a concentration-dependent inhibition of lorica formation with both dyes. In the presence of the inhibitors malformed loricae are made, which do not show the usual ultrastructure and arrangement of the chitin microfibrils. Instead of long, laterally associated microfibrils, short rods or irregular networks of subelementary (15-25 A) fibrils are found. Microfibril assembly obviously takes place on the accessible outside of the plasma membrane. There must be a gap between the polymerization and microfibril formation reactions, allowing the stains to bind to the polymerized subunits. Thus, later association of these units to form microfibrils is disturbed. The microfibril-orienting mechanism also depends on normal microfibril formation. A model summarizing these hypotheses is suggested.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Blackwell J, Parker KD, Rudall KM. Chitin fibres of the diatoms Thalassiosira fluviatilis and Cyclotella cryptica. J Mol Biol. 1967 Sep 14;28(2):383–385. [PubMed]
  • Giddings TH, Jr, Brower DL, Staehelin LA. Visualization of particle complexes in the plasma membrane of Micrasterias denticulata associated with the formation of cellulose fibrils in primary and secondary cell walls. J Cell Biol. 1980 Feb;84(2):327–339. [PMC free article] [PubMed]
  • Harrington BJ, Raper KB. Use of a fluorescent brightener to demonstrate cellulose in the cellular slime molds. Appl Microbiol. 1968 Jan;16(1):106–113. [PMC free article] [PubMed]
  • Heath IB. A unified hypothesis for the role of membrane bound enzyme complexes and microtubules in plant cell wall synthesis. J Theor Biol. 1974 Dec;48(2):445–449. [PubMed]
  • Herth W, Kuppel A, Schnepf E. Chitinous fibrils in the lorica of the flagellate chrysophyte Poteriochromonas stipitata (syn. Ochromonas malhamensis). J Cell Biol. 1977 May;73(2):311–321. [PMC free article] [PubMed]
  • Herth W, Zugenmaier P. Ultrastructure of the chitin fibrils of the centric diatom Cyclotella cryptica. J Ultrastruct Res. 1977 Nov;61(2):230–239. [PubMed]
  • Herth W, Barthlott W. The site of beta-chitin fibril formation in centric diatoms. I. Pores and fibril formation. J Ultrastruct Res. 1979 Jul;68(1):6–15. [PubMed]
  • Herth W. The site of beta-chitin fibril formation in centric diatoms. II. The chitin-forming cytoplasmic structures. J Ultrastruct Res. 1979 Jul;68(1):16–27. [PubMed]
  • Leppard GG. Nascent stage of cellulose biosynthesis. Science. 1975 Sep 26;189(4208):1094–1095. [PubMed]
  • Mueller SC, Brown RM., Jr Evidence for an intramembrane component associated with a cellulose microfibril-synthesizing complex in higher plants. J Cell Biol. 1980 Feb;84(2):315–326. [PMC free article] [PubMed]

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