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J Cell Biol. May 1, 1973; 57(2): 359–372.
PMCID: PMC2108974

INDEPENDENCE OF CENTRIOLE FORMATION AND DNA SYNTHESIS

Abstract

The temporal relationship between cell cycle events and centriole duplication was investigated electron microscopically in L cells synchronized by mechanically selecting mitotic cells. The two mature centrioles which each cell received at telophase migrated together from the side of the telophase nucleus distal to the stem body around to a region of the cytoplasm near the stem body and then into a groovelike indention in the early G1 nucleus, where they were found throughout interphase. Procentrioles appeared in association with each mature centriole at times varying from 4 to 12 h after mitosis. Since S phase was found to begin on the average about 9 h after mitotic selection, it appeared that cells generated procentrioles late in G1 or early in S. During prophase, the two centriolar duplexes migrated to opposite sides of the nucleus and the daughter centrioles elongated to the mature length. To ascertain whether any aspect of centriolar duplication was contingent upon nuclear DNA synthesis, arabinosyl cytosine was added to mitotic cells at a concentration which inhibited cellular DNA synthesis by more than 99%. Though cells were thus prevented from entering S phase, the course of procentriole formation was not detectibly affected. However, cells were inhibited from proceeding to the next mitosis, and the centriolar elongation and migration normally associated with prophase did not occur.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Brinkley BR, Murphy P, Richardson LC. Procedure for embedding in situ selected cells cultured in vitro. J Cell Biol. 1967 Oct;35(1):279–283. [PMC free article] [PubMed]
  • Hotta Y, Ito M, Stern H. Synthesis of DNA during meiosis. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1184–1191. [PMC free article] [PubMed]
  • Murray RG, Murray AS, Pizzo A. The fine structure of mitosis in rat thymic lymphocytes. J Cell Biol. 1965 Aug;26(2):601–619. [PMC free article] [PubMed]
  • Nass MM. Mitochondrial DNA. I. Intramitochondrial distribution and structural relations of single- and double-length circular DNA. J Mol Biol. 1969 Jun 28;42(3):521–528. [PubMed]
  • Rattner JB. Observations of centriole formation in male meiosis. J Cell Biol. 1972 Jul;54(1):20–29. [PMC free article] [PubMed]
  • Robbins E, Jentzsch G, Micali A. The centriole cycle in synchronized HeLa cells. J Cell Biol. 1968 Feb;36(2):329–339. [PMC free article] [PubMed]
  • SOROKIN S. Centrioles and the formation of rudimentary cilia by fibroblasts and smooth muscle cells. J Cell Biol. 1962 Nov;15:363–377. [PMC free article] [PubMed]
  • TERASIMA T, TOLMACH LJ. Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells. Exp Cell Res. 1963 Apr;30:344–362. [PubMed]
  • Younger KB, Banerjee S, Kelleher JK, Winston M, Margulis L. Evidence that the synchronized production of new basal bodies is not associated with DNA synthesis in Stentor coeruleus. J Cell Sci. 1972 Sep;11(2):621–637. [PubMed]

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