Logo of jcellbiolHomeThe Rockefeller University PressEditorsContactInstructions for AuthorsThis issue
J Cell Biol. 1972 Dec 1; 55(3): 579–585.
PMCID: PMC2108819


I. Separation and Partial Synchrony of Mitotic Cells


Using a colloidal silica density gradient, HeLa cells in mitosis were found to have a density of 1.040–1.046 g/cc, lighter than the remaining interphase cells. The mitotic cells could be harvested and cultured after centrifugation, showing growth synchrony by measurement of a peak in mitotic index 21 hr after establishing the culture. By using Colcemid or vinblastine sulfate, HeLa cells were arrested in metaphase and centrifuged on the colloidal silica density gradient. The blocked metaphase cells were lighter in density than the interphase cells but somewhat more dense than untreated cells selected by the density gradient centrifugation. Near-equilibrium conditions were established during the centrifugation of cells so that cell density measurements could be made, and the gradient medium employed was not measurably toxic to those cells tested.

Full Text

The Full Text of this article is available as a PDF (400K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Whitmore GF. Natural and induced synchronous cultures. In Vitro. 1971 Jan-Feb;6(4):276–285. [PubMed]
  • AXELRAD AA, McCULLOCH EA. Obtaining suspensions of animal cells in metaphase from cultures propagated on glass. Stain Technol. 1958 Mar;33(2):67–71. [PubMed]
  • TERASIMA T, TOLMACH LJ. Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells. Exp Cell Res. 1963 Apr;30:344–362. [PubMed]
  • Lindahl PE, Sörenby L. A new method for the continuous selection of cells in mitosis. Exp Cell Res. 1966 Sep;43(2):424–434. [PubMed]
  • Schindler R, Ramseier L, Schaer JC, Grieder A. Studies on the division cycle of mammalian cells. 3. Preparation of synchronously dividing cell populations by isotonic sucrose gradient centrifugation. Exp Cell Res. 1970 Jan;59(1):90–96. [PubMed]
  • Morris NR, Cramer JW, Reno D. A simple method for concentration of cells in the DNA synthetic period of the mitotic cycle. Exp Cell Res. 1967 Oct;48(1):216–218. [PubMed]
  • Warmsley AM, Pasternak CA. The use of conventional and zonal centrifugation to study the life cycle of mammalian cells. Phospholipid and macromolecular synthesis in neoplastic mast cells. Biochem J. 1970 Sep;119(3):493–499. [PMC free article] [PubMed]
  • EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. [PubMed]
  • Stubblefield E, Klevecz R. Synchronization of Chinese hamster cells by reversal of colcemid inhibition. Exp Cell Res. 1965 Dec;40(3):660–664. [PubMed]
  • Kim JH, Stambuk BK. Synchronization of HeLa cells by vinblastine sulfate. Exp Cell Res. 1966 Nov-Dec;44(2):631–634. [PubMed]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press


Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Compound
    PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...