Logo of jcellbiolHomeThe Rockefeller University PressEditorsContactInstructions for AuthorsThis issue
J Cell Biol. 1969 Oct 1; 43(1): 105–114.
PMCID: PMC2107846


Composition and Effects of Phagocytosis on Incorporation of Radioactive Precursors


The lipids of Acanthamoeba castellanii (Neff) consist of 52% neutral lipids and 48% polar lipids. Triglycerides account for 75% and free sterols for 17% of the neutral lipids. The major phospholipids are phosphatidylcholine (45%), phosphatidylethanolamine (33%), phosphatidylserine (10%), a phosphoinositide (6%), and diphosphatidylglycerol (4%). The phosphoinositide is unique in that it contains fatty acids, aldehyde, inositol, and phosphate in the ratio of 1.4:0.5:1.1, but it contains no glycerol. Sphingomyelin, cerebrosides, psychosine, and glycoglycerides were not detected, but small amounts of unidentified long chain bases and sugars are present. The rates of uptake of palmitate-1-14C and of its incorporation into glycerides and phospholipids were not affected by the phagocytosis of polystyrene latex beads. Although phagocytosis usually decreased the uptake by amebas of phosphate-32P, serine-U-14C, and inositol-2-3H, their subsequent incroporation into phospholipids was not demonstrably stimulated or inhibited by phagocytosis. Phagocytosis did seem to increase the incorporation into ameba phospholipids of phosphatidylcholine-1 ,2-14C but not that of phosphatidylethanolamine-1 ,2-14C. These experiments, in which the incorporation of radioactive precursors into total cell lipids was measured, do not, of course, eliminate the possibility that localized effects may occur.

Full Text

The Full Text of this article is available as a PDF (668K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Weisman RA, Korn ED. Phagocytosis of latex beads by Acanthamoeba. I. Biochemical properties. Biochemistry. 1967 Feb;6(2):485–497. [PubMed]
  • Korn ED, Weisman RA. Phagocytosis of latex beads by Acanthamoeba. II. Electron microscopic study of the initial events. J Cell Biol. 1967 Jul;34(1):219–227. [PMC free article] [PubMed]
  • Wetzel MG, Korn ED. Phagocytosis of latex beads by Acahamoeba castellanii (Neff). 3. Isolation of the phagocytic vesicles and their membranes. J Cell Biol. 1969 Oct;43(1):90–104. [PMC free article] [PubMed]
  • GOODMAN DS. Preparation of human serum albumin free of long-chain fatty acids. Science. 1957 Jun 28;125(3261):1296–1297. [PubMed]
  • Weisman RA, Korn ED. Uptake of fatty acids by Acanthamoeba. Biochim Biophys Acta. 1966 Apr 4;116(2):229–242. [PubMed]
  • FOLCH J, LEES M, SLOANE STANLEY GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed]
  • Biezenski JJ. Efficient elution of rabbit liver and plasma phospholipids from thin-layer plates. J Lipid Res. 1967 Jul;8(4):409–410. [PubMed]
  • MOORE PR, BAUMANN CA. Skin sterols. I. Colorimetric determination of cholesterol and other sterols in skin. J Biol Chem. 1952 Apr;195(2):615–621. [PubMed]
  • Smith FR, Korn ED. 7-Dehydrostigmasterol and ergosterol: the major sterols of an amoeba. J Lipid Res. 1968 Jul;9(4):405–408. [PubMed]
  • RAPPORT MM, ALONZO N. Photometric determination of fatty acid ester groups in phospholipides. J Biol Chem. 1955 Nov;217(1):193–198. [PubMed]
  • RAPPORT MM, ALONZO N. Identification of phosphatidal choline as the major constituent of beef heart lecithin. J Biol Chem. 1955 Nov;217(1):199–204. [PubMed]
  • ROSEN H. A modified ninhydrin colorimetric analysis for amino acids. Arch Biochem Biophys. 1957 Mar;67(1):10–15. [PubMed]
  • GRANT DR. Reagent stability in Rosen's ninhydrin method of analysis for amino acids. Anal Biochem. 1963 Jul;6:109–110. [PubMed]
  • AXELROD J, REICHENTHAL J, BRODIE BB. The direct determination of phosphatidyl ethanolamine and phosphatidyl serine in plasma and red blood cells. J Biol Chem. 1953 Oct;204(2):903–911. [PubMed]
  • SPENCER B. The ultramicro determination of inorganic sulphte. Biochem J. 1960 Jun;75:435–440. [PMC free article] [PubMed]
  • WHEELDON LW, COLLINS FD. Studies on phospholipids. 3. Determination of choline. Biochem J. 1958 Sep;70(1):43–45. [PMC free article] [PubMed]
  • HANAHAN DJ, OLLEY JN. Chemical nature of monophosphoinositides. J Biol Chem. 1958 Apr;231(2):813–828. [PubMed]
  • Walborg EF, Jr, Christensson L. A colorimetric method for the quantitative determination of monosaccharides. Anal Biochem. 1965 Nov;13(2):186–193. [PubMed]
  • CRANE RK, LIPMANN F. The effect of arsenate on aerobic phosphorylation. J Biol Chem. 1953 Mar;201(1):235–243. [PubMed]
  • KORN ED. FATTY ACIDS OF ACANTHAMOEBA SP. J Biol Chem. 1963 Nov;238:3584–3587. [PubMed]
  • SBARRA AJ, KARNOVSKY ML. The biochemical basis of phagocytosis. 2. Incorporation of C14-labeled building blocks into lipid, protein, and glycogen of leukocytes during phagocytosis. J Biol Chem. 1960 Aug;235:2224–2229. [PubMed]
  • KARNOVSKY ML, WALLACH DF. The metabolic basis of phagocytosis. III. Incorporation of inorganic phosphate into various classes of phosphatides during phagocytosis. J Biol Chem. 1961 Jul;236:1895–1901. [PubMed]
  • OREN R, FARNHAM AE, SAITO K, MILOFSKY E, KARNOVSKY ML. Metabolic patterns in three types of phagocytizing cells. J Cell Biol. 1963 Jun;17:487–501. [PMC free article] [PubMed]
  • Sastry PS, Hokin LE. Studies on the role of phospholipids in phagocytosis. J Biol Chem. 1966 Jul 25;241(14):3354–3361. [PubMed]
  • Graham RC, Jr, Karnovsky MJ, Shafer AW, Glass EA, Karnovsky ML. Metabolic and morphological observations on the effect of surface-active agents of leukocytes. J Cell Biol. 1967 Mar;32(3):629–647. [PMC free article] [PubMed]
  • Elsbach P, Levy S. Increased synthesis of phospholipid during phagocytosis. J Clin Invest. 1968 Oct;47(10):2217–2229. [PMC free article] [PubMed]
  • Prottey C, Ballou CE. Diacyl myoinositol monomannoside from Propionibacterium shermanii. J Biol Chem. 1968 Dec 10;243(23):6196–6201. [PubMed]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press


Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Compound
    PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.
  • Taxonomy
    Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
  • Taxonomy Tree
    Taxonomy Tree

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...