Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Jan 1988; 170(1): 149–154.
PMCID: PMC210619

Identification of a Staphylococcus aureus transposon (Tn4291) that carries the methicillin resistance gene(s).


We isolated a transposon (Tn4291) that carries the resistance gene(s) for methicillin in a secondary insertion site on the penicillinase plasmid pI524. Transposition of Tn4291 into pI524 occurred during the transduction of the tetracycline resistance plasmid pSN1 from a methicillin-resistant donor into a recipient that carried the mec allele in the primary site on the chromosome. Insertion of Tn4291 caused extensive rearrangement of pI524 and resulted in the formation of a 27.9-kilobase-pair plasmid (pIT103) which coded for resistance to methicillin and cadmium, but not penicillin. Although resistance to methicillin and cadmium were always linked, Tn4291 was stably maintained only in the presence of a chromosomal mec allele, while in its absence the plasmid was unstable and transposition to the primary site occurred. Subsequently, a 20.1-kilobase-pair plasmid, pIT203, was formed which retained cadmium resistance and regained the ability to express beta-lactamase activity.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Annear DI, Grubb WB. Methicillin-sensitive variants in ageing broth cultures of methicillin-resistant Staphylococcus aureus. Pathology. 1976 Jan;8(1):69–72. [PubMed]
  • Chang S, Cohen SN. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA. Mol Gen Genet. 1979 Jan 5;168(1):111–115. [PubMed]
  • Cohen S, Sweeney HM. Transduction of Methicillin Resistance in Staphylococcus aureus Dependent on an Unusual Specificity of the Recipient Strain. J Bacteriol. 1970 Dec;104(3):1158–1167. [PMC free article] [PubMed]
  • Dornbusch K, Hallander HO. Transduction of penicillinase production and methicillin resistance-enterotoxin B production in strains of Staphylococcus aureus. J Gen Microbiol. 1973 May;76(1):1–11. [PubMed]
  • Dornbusch K, Hallander HO, Löfquist F. Extrachromosomal control of methicillin resistance and toxin production in Staphylococcus aureus. J Bacteriol. 1969 May;98(2):351–358. [PMC free article] [PubMed]
  • Dyer DW, Iandolo JJ. Rapid isolation of DNA from Staphylococcus aureus. Appl Environ Microbiol. 1983 Jul;46(1):283–285. [PMC free article] [PubMed]
  • Dyer DW, Rock MI, Iandolo JJ. Autogenous transduction of phi 11de in Staphylococcus aureus: transfer and genetic properties. J Bacteriol. 1984 May;158(2):689–695. [PMC free article] [PubMed]
  • Dyer DW, Rock MI, Lee CY, Iandolo JJ. Generation of transducing particles in Staphylococcus aureus. J Bacteriol. 1985 Jan;161(1):91–95. [PMC free article] [PubMed]
  • Gillespie MT, May JW, Skurray RA. Detection of an integrated tetracycline resistance plasmid in the chromosome of methicillin-resistant Staphylococcus aureus. J Gen Microbiol. 1986 Jun;132(6):1723–1728. [PubMed]
  • Kayser FH, Wüst J, Santanam P. Genetic and molecular characterisation of resistance determinants in methicillin-resistant Staphylococcus-aureus. J Med Microbiol. 1976 May;9(2):137–148. [PubMed]
  • Kuhl SA, Pattee PA, Baldwin JN. Chromosomal map location of the methicillin resistance determinant in Staphylococcus aureus. J Bacteriol. 1978 Aug;135(2):460–465. [PMC free article] [PubMed]
  • Lacey RW. Genetic control in methicillin-resistant strains of Staphylococcus aureus. J Med Microbiol. 1972 Nov;5(4):497–508. [PubMed]
  • Lacey RW, Grinsted J. Genetic analysis of methicillin-resistant strains of Staphylococcus aureus; evidence for their evolution from a single clone. J Med Microbiol. 1973 Nov;6(4):511–526. [PubMed]
  • Landy A, Ross W. Viral integration and excision: structure of the lambda att sites. Science. 1977 Sep 16;197(4309):1147–1160. [PMC free article] [PubMed]
  • Lee CY, Iandolo JJ. Mechanism of bacteriophage conversion of lipase activity in Staphylococcus aureus. J Bacteriol. 1985 Oct;164(1):288–293. [PMC free article] [PubMed]
  • Lyon BR, Skurray R. Antimicrobial resistance of Staphylococcus aureus: genetic basis. Microbiol Rev. 1987 Mar;51(1):88–134. [PMC free article] [PubMed]
  • Murphy E. Inhibition of Tn554 transposition: deletion analysis. Plasmid. 1983 Nov;10(3):260–269. [PubMed]
  • Murphy E, Löfdahl S. Transposition of Tn554 does not generate a target duplication. Nature. 1984 Jan 19;307(5948):292–294. [PubMed]
  • Novick RP, Murphy E, Gryczan TJ, Baron E, Edelman I. Penicillinase plasmids of Staphylococcus aureus: restriction-deletion maps. Plasmid. 1979 Jan;2(1):109–129. [PubMed]
  • Phillips S, Novick RP. Tn554--a site-specific repressor-controlled transposon in Staphylococcus aureus. Nature. 1979 Mar 29;278(5703):476–478. [PubMed]
  • Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. [PubMed]
  • Shafer WM, Iandolo JJ. Genetics of staphylococcal enterotoxin B in methicillin-resistant isolates of Staphylococcus aureus. Infect Immun. 1979 Sep;25(3):902–911. [PMC free article] [PubMed]
  • Shafer WM, Iandolo JJ. Transduction of staphylococcal enterotoxin B synthesis: establishment of the toxin gene in a recombination-deficient mutant. Infect Immun. 1980 Jan;27(1):280–282. [PMC free article] [PubMed]
  • Shafferman A, Shalita Z, Hertman I. Cleavage maps of a tetracycline plasmid from Staphylococcus aureus. J Bacteriol. 1978 Apr;134(1):345–348. [PMC free article] [PubMed]
  • Shalita Z, Murphy E, Novick RP. Penicillinase plasmids of Staphylococcus aureus: structural and evolutionary relationships. Plasmid. 1980 May;3(3):291–311. [PubMed]
  • Sjöström JE, Löfdahl S, Philipson L. Transformation reveals a chromosomal locus of the gene(s) for methicillin resistance in Staphylococcus aureus. J Bacteriol. 1975 Sep;123(3):905–915. [PMC free article] [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Stahl ML, Pattee PA. Computer-assisted chromosome mapping by protoplast fusion in Staphylococcus aureus. J Bacteriol. 1983 Apr;154(1):395–405. [PMC free article] [PubMed]
  • Stewart GC, Rosenblum ED. Transduction of methicillin resistance in Staphylococcus aureus: recipient effectiveness and beta-lactamase production. Antimicrob Agents Chemother. 1980 Sep;18(3):424–432. [PMC free article] [PubMed]
  • Stobberingh EE, Winkler KC. Restriction-deficient mutants of Staphylococcus aureus. J Gen Microbiol. 1977 Apr;99(2):359–367. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...