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J Cell Biol. Aug 1, 1962; 14(2): 193–205.
PMCID: PMC2106096

OBSERVATIONS ON THE FINE STRUCTURE OF THE DEVELOPING SPERMATID IN THE DOMESTIC CHICKEN

Abstract

The kinetic apparatus, the acrosome and associated structures, and the manchette of the spermatid of the domestic chicken have been studied with the electron microscope. The basic structural features of the two centrioles do not change during spermiogenesis, but there is a change in orientation and length. The proximal centriole is situated in a groove at the edge of the nucleus and oriented normal to the long axis of the nucleus and at right angles to the elongate distal centriole. The tail filaments appear to originate from the distal centriole. The plasma membrane is invaginated along the tail filaments. A dense structure which appears at the deep reflection of the plasma membrane is identified as the ring. The fine structure of the ring has no resemblance to that of a centriole and there is no evidence that it is derived from or related to the centrioles. The tail of the spermatid contains nine peripheral pairs and one central pair of tubular filaments. The two members of each pair of peripheral filaments differ in density and in shape: one is dense and circular, and the other is light and semilunar in cross-section. The dense filaments have processes. A manchette consisting of fine tubules appears in the cytoplasm of the older spermatid along the nucleus, neck region, and proximal segment of the tail. The acrosome is spherical in young spermatids and becomes crescentic and, finally, U-shaped as spermiogenesis proceeds. A dense granule is observed in the cytoplasm between acrosome and nucleus. This granule later becomes a dense rod which is interpreted as the perforatorium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • AFZELIUS B. Electron microscopy of the sperm tail; results obtained with a new fixative. J Biophys Biochem Cytol. 1959 Mar 25;5(2):269–278. [PMC free article] [PubMed]
  • AFZELIUS BA. The fine structure of the cilia from ctenophore swimming-plates. J Biophys Biochem Cytol. 1961 Feb;9:383–394. [PMC free article] [PubMed]
  • BENNETT HS, LUFT JH. zeta-Collidine as a basis for buffering fixatives. J Biophys Biochem Cytol. 1959 Aug;6(1):113–114. [PMC free article] [PubMed]
  • BURGOS MH, FAWCETT DW. Studies on the fine structure of the mammalian testis. I. Differentiation of the spermatids in the cat (Felis domestica). J Biophys Biochem Cytol. 1955 Jul 25;1(4):287–300. [PMC free article] [PubMed]
  • BURGOS MH, FAWCETT DW. An electron microscope study of spermatid differentiation in the toad, Bufo arenarum Hensel. J Biophys Biochem Cytol. 1956 May 25;2(3):223–240. [PMC free article] [PubMed]
  • GALTSOFF PS, PHILPOTT DE. Ultrastructure of the spermatozoon of the oyster. Crassostrea virginica. J Ultrastruct Res. 1960 Feb;3:241–253. [PubMed]
  • GIBBONS IR, GRIMSTONE AV. On flagellar structure in certain flagellates. J Biophys Biochem Cytol. 1960 Jul;7:697–716. [PMC free article] [PubMed]
  • LANSING AI, LAMY F. Fine structure of the cilia of rotifers. J Biophys Biochem Cytol. 1961 Apr;9:799–812. [PMC free article] [PubMed]
  • LUFT JH. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. [PMC free article] [PubMed]
  • RHODIN J, DALHAMN T. Electron microscopy of the tracheal ciliated mucosa in rat. Z Zellforsch Mikrosk Anat. 1956;44(4):345–412. [PubMed]
  • SOTELO JR, TRUJILLO-CENOZ O. Electron microscope study of the kinetic apparatus in animal sperm cells. Z Zellforsch Mikrosk Anat. 1958;48(5):565–601. [PubMed]
  • SOTELO JR, TRUJILLO-CENOZ O. Electron microscope study on the development of ciliary components of the neural epithelium of the chick embryo. Z Zellforsch Mikrosk Anat. 1958;49(1):1–12. [PubMed]
  • WATSON ML. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol. 1958 Jul 25;4(4):475–478. [PMC free article] [PubMed]
  • WATSON ML. Staining of tissue sections for electron microscopy with heavy metals. II. Application of solutions containing lead and barium. J Biophys Biochem Cytol. 1958 Nov 25;4(6):727–730. [PMC free article] [PubMed]
  • WOOD RL, HOWARD CC. Use of fine grain positive sheet film for electron microscopy. J Biophys Biochem Cytol. 1959 Jan 25;5(1):181–182. [PMC free article] [PubMed]

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