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J Bacteriol. Nov 1989; 171(11): 6383–6386.
PMCID: PMC210518

Trehalose accumulation in vegetative cells and spores of Myxococcus xanthus.

Abstract

The disaccharide trehalose is found in the spores and cysts of a variety of organisms. We analyzed developing cells of Myxococcus xanthus for trehalose accumulation. Vegetative cells grown in media with low osmotic strengths contained less than 5 micrograms of trehalose per mg of protein. Spores formed in fruiting bodies accumulated up to 1,100 micrograms of trehalose per mg of protein. Spores formed in liquid culture following the addition of glycerol contained up to 300 micrograms of trehalose per mg of protein. The trehalose contents of both spore types decreased rapidly during the early stages of germination. Trehalase activity was not detected in extracts of dormant or germinating spores. Trehalose accumulation in M. xanthus was also associated with elevated osmotic strength. Vegetative cells accumulated up to 214 micrograms of trehalose per mg of protein when grown in media containing elevated levels of solutes.

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Selected References

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  • Crowe JH, Crowe LM, Chapman D. Preservation of membranes in anhydrobiotic organisms: the role of trehalose. Science. 1984 Feb 17;223(4637):701–703. [PubMed]
  • Crowe JH, Crowe LM, Chapman D. Infrared spectroscopic studies on interactions of water and carbohydrates with a biological membrane. Arch Biochem Biophys. 1984 Jul;232(1):400–407. [PubMed]
  • Crowe JH, Whittam MA, Chapman D, Crowe LM. Interactions of phospholipid monolayers with carbohydrates. Biochim Biophys Acta. 1984 Jan 11;769(1):151–159. [PubMed]
  • DWORKIN M, GIBSON SM. A SYSTEM FOR STUDYING MICROBIAL MORPHOGENESIS: RAPID FORMATION OF MICROCYSTS IN MYXOCOCCUS XANTHUS. Science. 1964 Oct 9;146(3641):243–244. [PubMed]
  • Elbein AD. The metabolism of alpha,alpha-trehalose. Adv Carbohydr Chem Biochem. 1974;30:227–256. [PubMed]
  • Hagen DC, Bretscher AP, Kaiser D. Synergism between morphogenetic mutants of Myxococcus xanthus. Dev Biol. 1978 Jun;64(2):284–296. [PubMed]
  • Inouye M, Inouye S, Zusman DR. Biosynthesis and self-assembly of protein S, a development-specific protein of Myxococcus xanthus. Proc Natl Acad Sci U S A. 1979 Jan;76(1):209–213. [PMC free article] [PubMed]
  • Larsen PI, Sydnes LK, Landfald B, Strøm AR. Osmoregulation in Escherichia coli by accumulation of organic osmolytes: betaines, glutamic acid, and trehalose. Arch Microbiol. 1987 Feb;147(1):1–7. [PubMed]
  • Lee CW, Waugh JS, Griffin RG. Solid-state NMR study of trehalose/1,2-dipalmitoyl-sn-phosphatidylcholine interactions. Biochemistry. 1986 Jul 1;25(13):3737–3742. [PubMed]
  • McBride MJ, Ensign JC. Effects of intracellular trehalose content on Streptomyces griseus spores. J Bacteriol. 1987 Nov;169(11):4995–5001. [PMC free article] [PubMed]
  • McBride MJ, Ensign JC. Metabolism of endogenous trehalose by Streptomyces griseus spores and by spores or cells of other actinomycetes. J Bacteriol. 1987 Nov;169(11):5002–5007. [PMC free article] [PubMed]
  • Sudo SZ, Dworkin M. Resistance of vegetative cells and microcysts of Myxococcus xanthus. J Bacteriol. 1969 Jun;98(3):883–887. [PMC free article] [PubMed]
  • Thevelein JM. Regulation of trehalose mobilization in fungi. Microbiol Rev. 1984 Mar;48(1):42–59. [PMC free article] [PubMed]
  • Watson BF, Dworkin M. Comparative intermediary metabolism of vegetative cells and microcysts of Myxococcus xanthus. J Bacteriol. 1968 Nov;96(5):1465–1473. [PMC free article] [PubMed]

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