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J Bacteriol. Sep 1989; 171(9): 4778–4784.
PMCID: PMC210279

Region of the streptococcal plasmid pMV158 required for conjugative mobilization.

Abstract

The nonconjugative streptococcal plasmid pMV158 can be mobilized by the conjugative streptococcal plasmid pIP501. We determined the sequence of the 1.1-kilobase EcoRI fragment of pMV158 to complete the DNA sequence of the plasmid. We showed that an open reading frame, mob (able to encode a polypeptide of 58,020 daltons), is required for mobilization of pMV158. An intergenic region present in the EcoRI fragment contains four lengthy palindromes that are found also in one or more of the staphylococcal plasmids pT181, pE194, and pUB110. One palindromic sequence, palD, which is common to all four plasmids, also appeared to be necessary for mobilization. Circumstantial evidence indicates that this sequence contains both an oriT site and the mob promoter. The Mob protein is homologous in its amino-terminal half to Pre proteins encoded by pT181 and pE194 that were shown by others to be essential for site-specific cointegrative plasmid recombination; their main biological function may be plasmid mobilization.

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Selected References

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  • Balganesh TS, Lacks SA. Plasmid vector for cloning in Streptococcus pneumoniae and strategies for enrichment for recombinant plasmids. Gene. 1984 Jul-Aug;29(1-2):221–230. [PubMed]
  • Balganesh TS, Lacks SA. Heteroduplex DNA mismatch repair system of Streptococcus pneumoniae: cloning and expression of the hexA gene. J Bacteriol. 1985 Jun;162(3):979–984. [PMC free article] [PubMed]
  • Ballester S, Lopez P, Alonso JC, Espinosa M, Lacks SA. Selective advantage of deletions enhancing chloramphenicol acetyltransferase gene expression in Streptococcus pneumoniae plasmids. Gene. 1986;41(2-3):153–163. [PubMed]
  • Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. [PMC free article] [PubMed]
  • Burdett V. Identification of tetracycline-resistant R-plasmids in Streptococcus agalactiae (group B). Antimicrob Agents Chemother. 1980 Nov;18(5):753–760. [PMC free article] [PubMed]
  • Clewell DB. Plasmids, drug resistance, and gene transfer in the genus Streptococcus. Microbiol Rev. 1981 Sep;45(3):409–436. [PMC free article] [PubMed]
  • Clewell DB, Helinski DR. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. [PMC free article] [PubMed]
  • Currier TC, Nester EW. Isolation of covalently closed circular DNA of high molecular weight from bacteria. Anal Biochem. 1976 Dec;76(2):431–441. [PubMed]
  • del Solar GH, Puyet A, Espinosa M. Initiation signals for the conversion of single stranded to double stranded DNA forms in the streptococcal plasmid pLS1. Nucleic Acids Res. 1987 Jul 24;15(14):5561–5580. [PMC free article] [PubMed]
  • Derbyshire KM, Willetts NS. Mobilization of the non-conjugative plasmid RSF1010: a genetic analysis of its origin of transfer. Mol Gen Genet. 1987 Jan;206(1):154–160. [PubMed]
  • Espinosa M, Lopez P, Perez-Ureña MT, Lacks SA. Interspecific plasmid transfer between Streptococcus pneumoniae and Bacillus subtilis. Mol Gen Genet. 1982;188(2):195–201. [PubMed]
  • Gennaro ML, Kornblum J, Novick RP. A site-specific recombination function in Staphylococcus aureus plasmids. J Bacteriol. 1987 Jun;169(6):2601–2610. [PMC free article] [PubMed]
  • Horaud T, Le Bouguenec C, Pepper K. Molecular genetics of resistance to macrolides, lincosamides and streptogramin B (MLS) in streptococci. J Antimicrob Chemother. 1985 Jul;16 (Suppl A):111–135. [PubMed]
  • Horinouchi S, Weisblum B. Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide, and streptogramin type B antibodies. J Bacteriol. 1982 May;150(2):804–814. [PMC free article] [PubMed]
  • Horodniceanu T, Bouanchaud DH, Bieth G, Chabbert YA. R plasmids in Streptococcus agalactiae (group B). Antimicrob Agents Chemother. 1976 Nov;10(5):795–801. [PMC free article] [PubMed]
  • Horowitz B, Deonier RC. Formation of delta tra F' plasmids: specific recombination at oriT. J Mol Biol. 1985 Nov 20;186(2):267–274. [PubMed]
  • Ikeda H, Aoki K, Naito A. Illegitimate recombination mediated in vitro by DNA gyrase of Escherichia coli: structure of recombinant DNA molecules. Proc Natl Acad Sci U S A. 1982 Jun;79(12):3724–3728. [PMC free article] [PubMed]
  • Khan SA, Novick RP. Complete nucleotide sequence of pT181, a tetracycline-resistance plasmid from Staphylococcus aureus. Plasmid. 1983 Nov;10(3):251–259. [PubMed]
  • Lacks S. Integration efficiency and genetic recombination in pneumococcal transformation. Genetics. 1966 Jan;53(1):207–235. [PMC free article] [PubMed]
  • Lacks SA, Lopez P, Greenberg B, Espinosa M. Identification and analysis of genes for tetracycline resistance and replication functions in the broad-host-range plasmid pLS1. J Mol Biol. 1986 Dec 20;192(4):753–765. [PubMed]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed]
  • McKenzie T, Hoshino T, Tanaka T, Sueoka N. The nucleotide sequence of pUB110: some salient features in relation to replication and its regulation. Plasmid. 1986 Mar;15(2):93–103. [PubMed]
  • McKenzie T, Hoshino T, Tanaka T, Sueoka N. Correction. A revision of the nucleotide sequence and functional map of pUB110. Plasmid. 1987 Jan;17(1):83–85. [PubMed]
  • Novick RP, Projan SJ, Rosenblum W, Edelman I. Staphylococcal plasmid cointegrates are formed by host- and phage-mediated general rec systems that act on short regions of homology. Mol Gen Genet. 1984;195(1-2):374–377. [PubMed]
  • Priebe SD, Hadi SM, Greenberg B, Lacks SA. Nucleotide sequence of the hexA gene for DNA mismatch repair in Streptococcus pneumoniae and homology of hexA to mutS of Escherichia coli and Salmonella typhimurium. J Bacteriol. 1988 Jan;170(1):190–196. [PMC free article] [PubMed]
  • Projan SJ, Novick R. Comparative analysis of five related Staphylococcal plasmids. Plasmid. 1988 May;19(3):203–221. [PubMed]
  • Puyet A, del Solar GH, Espinosa M. Identification of the origin and direction of replication of the broad-host-range plasmid pLS1. Nucleic Acids Res. 1988 Jan 11;16(1):115–133. [PMC free article] [PubMed]
  • Rosenberg M, Court D. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979;13:319–353. [PubMed]
  • Shine J, Dalgarno L. Determinant of cistron specificity in bacterial ribosomes. Nature. 1975 Mar 6;254(5495):34–38. [PubMed]
  • Smith MD, Guild WR. Improved method for conjugative transfer by filter mating of Streptococcus pneumoniae. J Bacteriol. 1980 Oct;144(1):457–459. [PMC free article] [PubMed]
  • Smith MD, Shoemaker NB, Burdett V, Guild WR. Transfer of plasmids by conjugation in Streptococcus pneumonias. Plasmid. 1980 Jan;3(1):70–79. [PubMed]
  • Stassi DL, Lopez P, Espinosa M, Lacks SA. Cloning of chromosomal genes in Streptococcus pneumoniae. Proc Natl Acad Sci U S A. 1981 Nov;78(11):7028–7032. [PMC free article] [PubMed]
  • Tanaka T, Sueoka N. Site-specific in vitro binding of plasmid pUB110 to Bacillus subtilis membrane fraction. J Bacteriol. 1983 Jun;154(3):1184–1194. [PMC free article] [PubMed]
  • Warren GJ, Twigg AJ, Sherratt DJ. ColE1 plasmid mobility and relaxation complex. Nature. 1978 Jul 20;274(5668):259–261. [PubMed]
  • Willetts N, Wilkins B. Processing of plasmid DNA during bacterial conjugation. Microbiol Rev. 1984 Mar;48(1):24–41. [PMC free article] [PubMed]

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