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J Bacteriol. 1989 Sep; 171(9): 4714–4717.
PMCID: PMC210271

An osmoregulated dipeptide in stressed Rhizobium meliloti.


One common mechanism of cellular adaptation to osmotic stress is the accumulation of organic solutes in the cytosol. We have used natural-abundance 13C nuclear magnetic resonance to identify all organic solutes that accumulate to significant levels in Rhizobium meliloti. Our studies led to the discovery of a new dipeptide, N-acetylglutaminylglutamine amide (NAGGN), which is accumulated during osmotic stress. Only rarely have peptides been shown to function in bacteria, and furthermore, this is the first example of a peptide playing a role in osmoregulation. Evidence for the biological role of NAGGN in osmotic-stress protection is presented.

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  • Borowitzka LJ, Demmerle S, Mackay MA, Norton RS. Carbon-13 nuclear magnetic resonance study of osmoregulation in a blue-green alga. Science. 1980 Nov 7;210(4470):650–651. [PubMed]
  • Chambers ST, Kunin CM. Osmoprotective activity for Escherichia coli in mammalian renal inner medulla and urine. Correlation of glycine and proline betaines and sorbitol with response to osmotic loads. J Clin Invest. 1987 Nov;80(5):1255–1260. [PMC free article] [PubMed]
  • Curatolo A, D'Arcangelo P, Lino A, Brancati A. Distribuzione dell'acido N-acetil-aspartil-glutammico nel nevrasse di mammiferi. Boll Soc Ital Biol Sper. 1964 Dec 31;40(24 Suppl):2136–2138. [PubMed]
  • DEKKER CA, STONE D, FRUTON JS. A peptide from a marine alga. J Biol Chem. 1949 Dec;181(2):719–729. [PubMed]
  • Fox GE, Stackebrandt E, Hespell RB, Gibson J, Maniloff J, Dyer TA, Wolfe RS, Balch WE, Tanner RS, Magrum LJ, et al. The phylogeny of prokaryotes. Science. 1980 Jul 25;209(4455):457–463. [PubMed]
  • Larsen PI, Sydnes LK, Landfald B, Strøm AR. Osmoregulation in Escherichia coli by accumulation of organic osmolytes: betaines, glutamic acid, and trehalose. Arch Microbiol. 1987 Feb;147(1):1–7. [PubMed]
  • Le Rudulier D, Strom AR, Dandekar AM, Smith LT, Valentine RC. Molecular biology of osmoregulation. Science. 1984 Jun 8;224(4653):1064–1068. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Measures JC. Role of amino acids in osmoregulation of non-halophilic bacteria. Nature. 1975 Oct 2;257(5525):398–400. [PubMed]
  • Meister A, Anderson ME. Glutathione. Annu Rev Biochem. 1983;52:711–760. [PubMed]
  • Saimi Y, Martinac B, Gustin MC, Culbertson MR, Adler J, Kung C. Ion channels in Paramecium, yeast and Escherichia coli. Trends Biochem Sci. 1988 Aug;13(8):304–309. [PubMed]
  • Smith LT, Pocard JA, Bernard T, Le Rudulier D. Osmotic control of glycine betaine biosynthesis and degradation in Rhizobium meliloti. J Bacteriol. 1988 Jul;170(7):3142–3149. [PMC free article] [PubMed]
  • Thurston JH, Hauhart RE, Naccarato EF. Taurine: possible role in osmotic regulation of mammalian heart. Science. 1981 Dec 18;214(4527):1373–1374. [PubMed]
  • Yancey PH, Clark ME, Hand SC, Bowlus RD, Somero GN. Living with water stress: evolution of osmolyte systems. Science. 1982 Sep 24;217(4566):1214–1222. [PubMed]
  • Zaczek R, Koller K, Cotter R, Heller D, Coyle JT. N-acetylaspartylglutamate: an endogenous peptide with high affinity for a brain "glutamate" receptor. Proc Natl Acad Sci U S A. 1983 Feb;80(4):1116–1119. [PMC free article] [PubMed]

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