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J Bacteriol. Aug 1989; 171(8): 4303–4314.
PMCID: PMC210205

Actively replicating nucleoids influence positioning of division sites in Escherichia coli filaments forming cells lacking DNA.


The positioning of constrictions in Escherichia coli filaments pinching off anucleate cells was analyzed by fluorescence microscopy of dnaX(Ts), dnaX(Ts) sfiA, dnaA46(Ts), gyrA(Am) supF(Ts), and gyrB(Ts) mutants. In filaments with actively replicating nucleoids, constrictions were positioned close to the nucleoid, whereas in nonreplicating filaments, positioning of constrictions within the anucleate region was nearly random. We conclude that constriction positioning depends in an unknown way on nucleoid replication activity.

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  • Henson JM, Chu H, Irwin CA, Walker JR. Isolation and characterization of dnaX and dnaY temperature-sensitive mutants of Escherichia coli. Genetics. 1979 Aug;92(4):1041–1059. [PMC free article] [PubMed]
  • Hirota Y, Jacob F, Ryter A, Buttin G, Nakai T. On the process of cellular division in Escherichia coli. I. Asymmetrical cell division and production of deoxyribonucleic acid-less bacteria. J Mol Biol. 1968 Jul 14;35(1):175–192. [PubMed]
  • Hussain K, Begg KJ, Salmond GP, Donachie WD. ParD: a new gene coding for a protein required for chromosome partitioning and septum localization in Escherichia coli. Mol Microbiol. 1987 Jul;1(1):73–81. [PubMed]
  • Inouye M. Pleiotropic effect of the rec A gene of Escherichia coli: uncoupling of cell division from deoxyribonucleic acid replication. J Bacteriol. 1971 May;106(2):539–542. [PMC free article] [PubMed]
  • Jaffé A, D'Ari R, Hiraga S. Minicell-forming mutants of Escherichia coli: production of minicells and anucleate rods. J Bacteriol. 1988 Jul;170(7):3094–3101. [PMC free article] [PubMed]
  • Jaffé A, D'Ari R, Norris V. SOS-independent coupling between DNA replication and cell division in Escherichia coli. J Bacteriol. 1986 Jan;165(1):66–71. [PMC free article] [PubMed]
  • Jones NC, Donachie WD. Chromosome replication, transcription and control of cell division in Escherichia coli. Nat New Biol. 1973 May 23;243(125):100–103. [PubMed]
  • Little JW, Mount DW. The SOS regulatory system of Escherichia coli. Cell. 1982 May;29(1):11–22. [PubMed]
  • Monk M, Gross J. Induction of prophage lambda in a mutant of E. coli K12 defective in initiation of DNA replication at high temperature. Mol Gen Genet. 1971;110(4):299–306. [PubMed]
  • Orr E, Fairweather NF, Holland IB, Pritchard RH. Isolation and characterisation of a strain carrying a conditional lethal mutation in the cou gene of Escherichia coli K12. Mol Gen Genet. 1979;177(1):103–112. [PubMed]
  • Schaus N, O'Day K, Peters W, Wright A. Isolation and characterization of amber mutations in gene dnaA of escherichia coli K-12. J Bacteriol. 1981 Feb;145(2):904–913. [PMC free article] [PubMed]
  • Taschner PE, Huls PG, Pas E, Woldringh CL. Division behavior and shape changes in isogenic ftsZ, ftsQ, ftsA, pbpB, and ftsE cell division mutants of Escherichia coli during temperature shift experiments. J Bacteriol. 1988 Apr;170(4):1533–1540. [PMC free article] [PubMed]
  • Trueba FJ, Woldringh CL. Changes in cell diameter during the division cycle of Escherichia coli. J Bacteriol. 1980 Jun;142(3):869–878. [PMC free article] [PubMed]
  • Woldringh CL, de Jong MA, van den Berg W, Koppes L. Morphological analysis of the division cycle of two Escherichia coli substrains during slow growth. J Bacteriol. 1977 Jul;131(1):270–279. [PMC free article] [PubMed]
  • Woldringh CL, Valkendurg JA, Pas E, Taschner PE, Huls P, Wientjes FB. Physiological and geometrical conditions for cell division in Escherichia coli. Ann Inst Pasteur Microbiol. 1985 Jan-Feb;136A(1):131–138. [PubMed]

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