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J Bacteriol. Aug 1989; 171(8): 4272–4280.
PMCID: PMC210201

Expression of Escherichia coli dnaA and mioC genes as a function of growth rate.

Abstract

The synthesis of specific cellular components related to the initiation process of DNA replication was correlated with changes in growth rate. The concentrations of DnaA protein and mioC mRNA were determined for cells grown at six different growth rates; both increased relative to either total protein or total RNA, respectively, as the growth rate increased. Expression from the chromosomal mioC promoter, which contains a DnaA protein-binding site, was not repressed when the DnaA protein concentration was increased and was not derepressed in a dnaA46 mutant at 42 degrees C. The mioC transcript had a characteristic mRNA-type half-life of 1.51 min.

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  • Aiba H, Adhya S, de Crombrugghe B. Evidence for two functional gal promoters in intact Escherichia coli cells. J Biol Chem. 1981 Nov 25;256(22):11905–11910. [PubMed]
  • Atlung T, Clausen ES, Hansen FG. Autoregulation of the dnaA gene of Escherichia coli K12. Mol Gen Genet. 1985;200(3):442–450. [PubMed]
  • Baracchini E, Bremer H. Stringent and growth control of rRNA synthesis in Escherichia coli are both mediated by ppGpp. J Biol Chem. 1988 Feb 25;263(6):2597–2602. [PubMed]
  • Berk AJ, Sharp PA. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977 Nov;12(3):721–732. [PubMed]
  • Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. [PMC free article] [PubMed]
  • Bramhill D, Kornberg A. Duplex opening by dnaA protein at novel sequences in initiation of replication at the origin of the E. coli chromosome. Cell. 1988 Mar 11;52(5):743–755. [PubMed]
  • Braun RE, O'Day K, Wright A. Autoregulation of the DNA replication gene dnaA in E. coli K-12. Cell. 1985 Jan;40(1):159–169. [PubMed]
  • Brunschede H, Dove TL, Bremer H. Establishment of exponential growth after a nutritional shift-up in Escherichia coli B/r: accumulation of deoxyribonucleic acid, ribonucleic acid, and protein. J Bacteriol. 1977 Feb;129(2):1020–1033. [PMC free article] [PubMed]
  • Buhk HJ, Messer W. The replication origin region of Escherichia coli: nucleotide sequence and functional units. Gene. 1983 Oct;24(2-3):265–279. [PubMed]
  • Carter-Muenchau P, Wolf RE., Jr Growth-rate-dependent regulation of 6-phosphogluconate dehydrogenase level mediated by an anti-Shine-Dalgarno sequence located within the Escherichia coli gnd structural gene. Proc Natl Acad Sci U S A. 1989 Feb;86(4):1138–1142. [PMC free article] [PubMed]
  • Casey J, Davidson N. Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide. Nucleic Acids Res. 1977;4(5):1539–1552. [PMC free article] [PubMed]
  • Clewell DB, Helinski DR. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. [PMC free article] [PubMed]
  • Cole JR, Nomura M. Translational regulation is responsible for growth-rate-dependent and stringent control of the synthesis of ribosomal proteins L11 and L1 in Escherichia coli. Proc Natl Acad Sci U S A. 1986 Jun;83(12):4129–4133. [PMC free article] [PubMed]
  • Cole JR, Olsson CL, Hershey JW, Grunberg-Manago M, Nomura M. Feedback regulation of rRNA synthesis in Escherichia coli. Requirement for initiation factor IF2. J Mol Biol. 1987 Dec 5;198(3):383–392. [PubMed]
  • de Wind N, Parren P, Stuitje AR, Meijer M. Evidence for the involvement of the 16kD gene promoter in initiation of chromosomal replication of Escherichia coli strains carrying a B/r-derived replication origin. Nucleic Acids Res. 1987 Jun 25;15(12):4901–4914. [PMC free article] [PubMed]
  • Donachie WD. Relationship between cell size and time of initiation of DNA replication. Nature. 1968 Sep 7;219(5158):1077–1079. [PubMed]
  • Dretzen G, Bellard M, Sassone-Corsi P, Chambon P. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Anal Biochem. 1981 Apr;112(2):295–298. [PubMed]
  • Elliott T, Kassavetis GA, Geiduschek EP. The complex pattern of transcription in the segment of the bacteriophage T4 genome containing three of the head protein genes. Virology. 1984 Dec;139(2):260–282. [PubMed]
  • FRANZEN JS, BINKLEY SB. Comparison of the acid-soluble nucleotides in Escherichia coli at different growth rates. J Biol Chem. 1961 Feb;236:515–519. [PubMed]
  • Freedman LP, Zengel JM, Archer RH, Lindahl L. Autogenous control of the S10 ribosomal protein operon of Escherichia coli: genetic dissection of transcriptional and posttranscriptional regulation. Proc Natl Acad Sci U S A. 1987 Sep;84(18):6516–6520. [PMC free article] [PubMed]
  • Fuller RS, Kornberg A. Purified dnaA protein in initiation of replication at the Escherichia coli chromosomal origin of replication. Proc Natl Acad Sci U S A. 1983 Oct;80(19):5817–5821. [PMC free article] [PubMed]
  • Fuller RS, Funnell BE, Kornberg A. The dnaA protein complex with the E. coli chromosomal replication origin (oriC) and other DNA sites. Cell. 1984 Oct;38(3):889–900. [PubMed]
  • Funnell BE, Baker TA, Kornberg A. In vitro assembly of a prepriming complex at the origin of the Escherichia coli chromosome. J Biol Chem. 1987 Jul 25;262(21):10327–10334. [PubMed]
  • Gielow A, Kücherer C, Kölling R, Messer W. Transcription in the region of the replication origin, oriC, of Escherichia coli: termination of asnC transcripts. Mol Gen Genet. 1988 Nov;214(3):474–481. [PubMed]
  • Guzman EC, Carrillo FJ, Jimenez-Sanchez A. Differential inhibition of the initiation of DNA replication in stringent and relaxed strains of Escherichia coli. Genet Res. 1988 Jun;51(3):173–177. [PubMed]
  • Hansen EB, Atlung T, Hansen FG, Skovgaard O, von Meyenburg K. Fine structure genetic map and complementation analysis of mutations in the dnaA gene of Escherichia coli. Mol Gen Genet. 1984;196(3):387–396. [PubMed]
  • Junker DE, Jr, Rokeach LA, Ganea D, Chiaramello A, Zyskind JW. Transcription termination within the Escherichia coli origin of DNA replication, oriC. Mol Gen Genet. 1986 Apr;203(1):101–109. [PubMed]
  • Kogoma T, von Meyenburg K. The origin of replication, oriC, and the dnaA protein are dispensable in stable DNA replication (sdrA) mutants of Escherichia coli K-12. EMBO J. 1983;2(3):463–468. [PMC free article] [PubMed]
  • Kohara Y, Tohdoh N, Jiang XW, Okazaki T. The distribution and properties of RNA primed initiation sites of DNA synthesis at the replication origin of Escherichia coli chromosome. Nucleic Acids Res. 1985 Oct 11;13(19):6847–6866. [PMC free article] [PubMed]
  • Kölling R, Lother H. AsnC: an autogenously regulated activator of asparagine synthetase A transcription in Escherichia coli. J Bacteriol. 1985 Oct;164(1):310–315. [PMC free article] [PubMed]
  • Kücherer C, Lother H, Kölling R, Schauzu MA, Messer W. Regulation of transcription of the chromosomal dnaA gene of Escherichia coli. Mol Gen Genet. 1986 Oct;205(1):115–121. [PubMed]
  • Løbner-Olesen A, Atlung T, Rasmussen KV. Stability and replication control of Escherichia coli minichromosomes. J Bacteriol. 1987 Jun;169(6):2835–2842. [PMC free article] [PubMed]
  • Lother H, Kölling R, Kücherer C, Schauzu M. dnaA protein-regulated transcription: effects on the in vitro replication of Escherichia coli minichromosomes. EMBO J. 1985 Feb;4(2):555–560. [PMC free article] [PubMed]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed]
  • Melefors O, von Gabain A. Site-specific endonucleolytic cleavages and the regulation of stability of E. coli ompA mRNA. Cell. 1988 Mar 25;52(6):893–901. [PubMed]
  • Nilsson G, Belasco JG, Cohen SN, von Gabain A. Growth-rate dependent regulation of mRNA stability in Escherichia coli. Nature. 1984 Nov 1;312(5989):75–77. [PubMed]
  • Nozaki N, Okazaki T, Ogawa T. In vitro transcription of the origin region of replication of the Escherichia coli chromosome. J Biol Chem. 1988 Oct 5;263(28):14176–14183. [PubMed]
  • Ogawa T, Baker TA, van der Ende A, Kornberg A. Initiation of enzymatic replication at the origin of the Escherichia coli chromosome: contributions of RNA polymerase and primase. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3562–3566. [PMC free article] [PubMed]
  • Ogden GB, Pratt MJ, Schaechter M. The replicative origin of the E. coli chromosome binds to cell membranes only when hemimethylated. Cell. 1988 Jul 1;54(1):127–135. [PubMed]
  • Oka A, Sugimoto K, Takanami M, Hirota Y. Replication origin of the Escherichia coli K-12 chromosome: the size and structure of the minimum DNA segment carrying the information for autonomous replication. Mol Gen Genet. 1980 Apr;178(1):9–20. [PubMed]
  • Ota Y, Kikuchi A, Cashel M. Gene expression of an Escherichia coli ribosomal RNA promoter fused to structural genes of the galactose operon. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5799–5803. [PMC free article] [PubMed]
  • Pedersen S, Bloch PL, Reeh S, Neidhardt FC. Patterns of protein synthesis in E. coli: a catalog of the amount of 140 individual proteins at different growth rates. Cell. 1978 May;14(1):179–190. [PubMed]
  • Rokeach LA, Kassavetis GA, Zyskind JW. RNA polymerase pauses in vitro within the Escherichia coli origin of replication at the same sites where termination occurs in vivo. J Biol Chem. 1987 May 25;262(15):7264–7272. [PubMed]
  • Rokeach LA, Zyskind JW. RNA terminating within the E. coli origin of replication: stringent regulation and control by DnaA protein. Cell. 1986 Aug 29;46(5):763–771. [PubMed]
  • Sarubbi E, Rudd KE, Cashel M. Basal ppGpp level adjustment shown by new spoT mutants affect steady state growth rates and rrnA ribosomal promoter regulation in Escherichia coli. Mol Gen Genet. 1988 Aug;213(2-3):214–222. [PubMed]
  • Schauzu MA, Kücherer C, Kölling R, Messer W, Lother H. Transcripts within the replication origin, oriC, of Escherichia coli. Nucleic Acids Res. 1987 Mar 25;15(6):2479–2497. [PMC free article] [PubMed]
  • Sekimizu K, Bramhill D, Kornberg A. ATP activates dnaA protein in initiating replication of plasmids bearing the origin of the E. coli chromosome. Cell. 1987 Jul 17;50(2):259–265. [PubMed]
  • Sekimizu K, Yung BY, Kornberg A. The dnaA protein of Escherichia coli. Abundance, improved purification, and membrane binding. J Biol Chem. 1988 May 25;263(15):7136–7140. [PubMed]
  • Sinden RR, Carlson JO, Pettijohn DE. Torsional tension in the DNA double helix measured with trimethylpsoralen in living E. coli cells: analogous measurements in insect and human cells. Cell. 1980 Oct;21(3):773–783. [PubMed]
  • Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC. Measurement of protein using bicinchoninic acid. Anal Biochem. 1985 Oct;150(1):76–85. [PubMed]
  • Sompayrac L, Maaloe O. Autorepressor model for control of DNA replication. Nat New Biol. 1973 Jan 31;241(109):133–135. [PubMed]
  • Tanaka M, Hiraga S. Negative control of oriC plasmid replication by transcription of the oriC region. Mol Gen Genet. 1985;200(1):21–26. [PubMed]
  • Walker JE, Gay NJ, Saraste M, Eberle AN. DNA sequence around the Escherichia coli unc operon. Completion of the sequence of a 17 kilobase segment containing asnA, oriC, unc, glmS and phoS. Biochem J. 1984 Dec 15;224(3):799–815. [PMC free article] [PubMed]
  • Wang QP, Kaguni JM. Transcriptional repression of the dnaA gene of Escherichia coli by dnaA protein. Mol Gen Genet. 1987 Oct;209(3):518–525. [PubMed]
  • Wu HY, Shyy SH, Wang JC, Liu LF. Transcription generates positively and negatively supercoiled domains in the template. Cell. 1988 May 6;53(3):433–440. [PubMed]
  • Yung BY, Kornberg A. Membrane attachment activates dnaA protein, the initiation protein of chromosome replication in Escherichia coli. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7202–7205. [PMC free article] [PubMed]

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