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J Bacteriol. 1990 Jun; 172(6): 3496–3499.
PMCID: PMC209165

Construction of broad-host-range plasmid vectors for easy visible selection and analysis of promoters.


We have constructed a series of broad-host-range plasmids which use "visual screens" to detect promoter activity. These plasmids contain the pMB1 and pRO1600 origins of replication and are capable of replicating in a wide range of gram-negative bacteria. The genes encoding beta-galactosidase and alkaline phosphatase from Escherichia coli and bacterial luciferase from Vibrio harveyi supply the promoterless indicator genes. The constructs were tested in E. coli and Pseudomonas aeruginosa.

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