• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Feb 1990; 172(2): 1014–1018.
PMCID: PMC208530

The GLN3 gene product is required for transcriptional activation of allantoin system gene expression in Saccharomyces cerevisiae.

Abstract

We show that mutation at the GLN3 locus results in decreased steady-state levels of DAL7, DUR1,2, CAR1, and URA3 mRNAs derived from cultures grown in the presence of inducer. Basal levels of these RNA species, however, were not significantly affected by a gln3 mutation. The GLN3 product appears to affect gene expression in two ways. The pleiotropic requirement of GLN3 for induced gene expression probably derives from the need of the GLN3 product for inducer uptake into the cell and its loss in gln3 mutants. We also demonstrate that transcriptional activation, mediated by the DAL5 and DAL7 upstream activation sequences, requires a functional GLN3 gene product. This observation identified transcriptional activation as the most likely point of GLN3 participation in the expression of allantoin system genes.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Benjamin PM, Wu JI, Mitchell AP, Magasanik B. Three regulatory systems control expression of glutamine synthetase in Saccharomyces cerevisiae at the level of transcription. Mol Gen Genet. 1989 Jun;217(2-3):370–377. [PubMed]
  • Buckholz RG, Cooper TG. Oxalurate induction of multiple URA3 transcripts in Saccharomyces cerevisiae. Mol Cell Biol. 1983 Nov;3(11):1889–1897. [PMC free article] [PubMed]
  • Cooper TG, Rai R, Yoo HS. Requirement of upstream activation sequences for nitrogen catabolite repression of the allantoin system genes in Saccharomyces cerevisiae. Mol Cell Biol. 1989 Dec;9(12):5440–5444. [PMC free article] [PubMed]
  • Courchesne WE, Magasanik B. Regulation of nitrogen assimilation in Saccharomyces cerevisiae: roles of the URE2 and GLN3 genes. J Bacteriol. 1988 Feb;170(2):708–713. [PMC free article] [PubMed]
  • Fried HM, Warner JR. Cloning of yeast gene for trichodermin resistance and ribosomal protein L3. Proc Natl Acad Sci U S A. 1981 Jan;78(1):238–242. [PMC free article] [PubMed]
  • Genbauffe FS, Cooper TG. Induction and repression of the urea amidolyase gene in Saccharomyces cerevisiae. Mol Cell Biol. 1986 Nov;6(11):3954–3964. [PMC free article] [PubMed]
  • Lacroute F. Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae. J Bacteriol. 1968 Mar;95(3):824–832. [PMC free article] [PubMed]
  • Messenguy F, Dubois E. Participation of transcriptional and post-transcriptional regulatory mechanisms in the control of arginine metabolism in yeast. Mol Gen Genet. 1983;189(1):148–156. [PubMed]
  • Mitchell AP, Magasanik B. Regulation of glutamine-repressible gene products by the GLN3 function in Saccharomyces cerevisiae. Mol Cell Biol. 1984 Dec;4(12):2758–2766. [PMC free article] [PubMed]
  • Mitchell AP, Magasanik B. Three regulatory systems control production of glutamine synthetase in Saccharomyces cerevisiae. Mol Cell Biol. 1984 Dec;4(12):2767–2773. [PMC free article] [PubMed]
  • Rai R, Genbauffe F, Lea HZ, Cooper TG. Transcriptional regulation of the DAL5 gene in Saccharomyces cerevisiae. J Bacteriol. 1987 Aug;169(8):3521–3524. [PMC free article] [PubMed]
  • Rai R, Genbauffe FS, Sumrada RA, Cooper TG. Identification of sequences responsible for transcriptional activation of the allantoate permease gene in Saccharomyces cerevisiae. Mol Cell Biol. 1989 Feb;9(2):602–608. [PMC free article] [PubMed]
  • Sumrada R, Cooper TG. Oxaluric acid: a non-metabolizable inducer of the allantoin degradative enzymes in Saccharomyces cerevisiae. J Bacteriol. 1974 Mar;117(3):1240–1247. [PMC free article] [PubMed]
  • Sumrada RA, Cooper TG. Urea carboxylase and allophanate hydrolase are components of a multifunctional protein in yeast. J Biol Chem. 1982 Aug 10;257(15):9119–9127. [PubMed]
  • Sumrada RA, Cooper TG. Isolation of the CAR1 gene from Saccharomyces cerevisiae and analysis of its expression. Mol Cell Biol. 1982 Dec;2(12):1514–1523. [PMC free article] [PubMed]
  • Turoscy V, Cooper TG. Pleiotropic control of five eucaryotic genes by multiple regulatory elements. J Bacteriol. 1982 Sep;151(3):1237–1246. [PMC free article] [PubMed]
  • Yoo HS, Cooper TG. The DAL7 promoter consists of multiple elements that cooperatively mediate regulation of the gene's expression. Mol Cell Biol. 1989 Aug;9(8):3231–3243. [PMC free article] [PubMed]
  • Yoo HS, Genbauffe FS, Cooper TG. Identification of the ureidoglycolate hydrolase gene in the DAL gene cluster of Saccharomyces cerevisiae. Mol Cell Biol. 1985 Sep;5(9):2279–2288. [PMC free article] [PubMed]
  • Zacharski CA, Cooper TG. Metabolite compartmentation in Saccharomyces cerevisiae. J Bacteriol. 1978 Aug;135(2):490–497. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...