• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Feb 1991; 173(4): 1554–1560.
PMCID: PMC207295

Characterization of the late-gene regulatory region of phage 21.

Abstract

A segment of Escherichia coli bacteriophage 21 DNA encoding the late-gene regulator, Q21, and the late-gene leader RNA segment was sequenced; its structure is similar to those of the related phages lambda and 82. The leader RNA is about 45 nucleotides long and consists essentially entirely of sequences encoding the p-independent terminator that is the putative target of the antitermination activity of Q21. Like the corresponding regions of lambda and 82, the 21 late-gene promoter segment encodes an early transcription pause in vitro, at about nucleotide 18, during which Q21 presumably acts to modify RNA polymerase. The 21 Q gene, cloned in isolation, is active on the late-gene leader segment in trans, and its purified product is active as an antiterminator in vitro; Q21 represents a third late-gene antiterminator, in addition to those of lambda and 82. There is little evident similarity in the primary sequences of the three Q genes.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.9M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Burgess RR, Jendrisak JJ. A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography. Biochemistry. 1975 Oct 21;14(21):4634–4638. [PubMed]
  • Donis-Keller H, Maxam AM, Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. [PMC free article] [PubMed]
  • Franklin NC. "N" transcription antitermination proteins of bacteriophages lambda, phi 21 and P22. J Mol Biol. 1985 Jan 5;181(1):85–91. [PubMed]
  • Goliger JA, Roberts JW. Bacteriophage 82 gene Q and Q protein. Sequence, overproduction, and activity as a transcription antiterminator in vitro. J Biol Chem. 1987 Aug 25;262(24):11721–11725. [PubMed]
  • Goliger JA, Roberts JW. Sequences required for antitermination by phage 82 Q protein. J Mol Biol. 1989 Dec 5;210(3):461–471. [PubMed]
  • Grayhack EJ, Roberts JW. Purification of the bacteriophage lambda late gene regulator encoded by gene Q. J Biol Chem. 1983 Aug 10;258(15):9192–9196. [PubMed]
  • Grayhack EJ, Yang XJ, Lau LF, Roberts JW. Phage lambda gene Q antiterminator recognizes RNA polymerase near the promoter and accelerates it through a pause site. Cell. 1985 Aug;42(1):259–269. [PubMed]
  • Lis JT. Fractionation of DNA fragments by polyethylene glycol induced precipitation. Methods Enzymol. 1980;65(1):347–353. [PubMed]
  • Lowe PA, Hager DA, Burgess RR. Purification and properties of the sigma subunit of Escherichia coli DNA-dependent RNA polymerase. Biochemistry. 1979 Apr 3;18(7):1344–1352. [PubMed]
  • Maxam AM, Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. [PMC free article] [PubMed]
  • McKenney K, Shimatake H, Court D, Schmeissner U, Brady C, Rosenberg M. A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase. Gene Amplif Anal. 1981;2:383–415. [PubMed]
  • Metcalf P, Blum M, Freymann D, Turner M, Wiley DC. Two variant surface glycoproteins of Trypanosoma brucei of different sequence classes have similar 6 A resolution X-ray structures. Nature. 1987 Jan 1;325(6099):84–86. [PubMed]
  • Pabo CO, Sauer RT. Protein-DNA recognition. Annu Rev Biochem. 1984;53:293–321. [PubMed]
  • Roberts JW. Phage lambda and the regulation of transcription termination. Cell. 1988 Jan 15;52(1):5–6. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Sauer RT, Yocum RR, Doolittle RF, Lewis M, Pabo CO. Homology among DNA-binding proteins suggests use of a conserved super-secondary structure. Nature. 1982 Jul 29;298(5873):447–451. [PubMed]
  • Schechtman MG, Snedeker JD, Roberts JW. Genetics and structure of the late gene regulatory region of phage 82. Virology. 1980 Sep;105(2):393–404. [PubMed]
  • Schleif R. The specificity of lamboid phage late gene induction (lamboid phage late gene specificity). Virology. 1972 Nov;50(2):610–612. [PubMed]
  • Takeda Y, Ohlendorf DH, Anderson WF, Matthews BW. DNA-binding proteins. Science. 1983 Sep 9;221(4615):1020–1026. [PubMed]
  • Yang XJ, Goliger JA, Roberts JW. Specificity and mechanism of antitermination by Q proteins of bacteriophages lambda and 82. J Mol Biol. 1989 Dec 5;210(3):453–460. [PubMed]
  • Yang XJ, Hart CM, Grayhack EJ, Roberts JW. Transcription antitermination by phage lambda gene Q protein requires a DNA segment spanning the RNA start site. Genes Dev. 1987 May;1(3):217–226. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...