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J Bacteriol. 1991 Feb; 173(3): 1353–1356.
PMCID: PMC207264

Efficient transformation of Bacillus thuringiensis requires nonmethylated plasmid DNA.


The transformation efficiency of Bacillus thuringiensis depends upon the source of plasmid DNA. DNA isolated from B. thuringiensis, Bacillus megaterium, or a Dam- Dcm- Escherichia coli strain efficiently transformed several B. thuringiensis strains, B. thuringiensis strains were grouped according to which B. thuringiensis backgrounds were suitable sources of DNA for transformation of other B. thuringiensis strains, suggesting that B. thuringiensis strains differ in DNA modification and restriction. Efficient transformation allowed the demonstration of developmental regulation of cloned crystal protein genes in B. thuringiensis.

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Selected References

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  • Alikhanian SI, Ryabchenko NF, Bukanov NO, Sakanyan VA. Transformation of Bacillus thuringiensis subsp. galleria protoplasts by plasmid pBC16. J Bacteriol. 1981 Apr;146(1):7–9. [PMC free article] [PubMed]
  • Belliveau BH, Trevors JT. Transformation of Bacillus cereus vegetative cells by electroporation. Appl Environ Microbiol. 1989 Jun;55(6):1649–1652. [PMC free article] [PubMed]
  • Bernhard K, Schrempf H, Goebel W. Bacteriocin and antibiotic resistance plasmids in Bacillus cereus and Bacillus subtilis. J Bacteriol. 1978 Feb;133(2):897–903. [PMC free article] [PubMed]
  • Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed]
  • Bone EJ, Ellar DJ. Transformation of Bacillus thuringiensis by electroporation. FEMS Microbiol Lett. 1989 Apr;49(2-3):171–177. [PubMed]
  • Casadaban MJ, Chou J, Cohen SN. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J Bacteriol. 1980 Aug;143(2):971–980. [PMC free article] [PubMed]
  • Crawford IT, Greis KD, Parks L, Streips UN. Facile autoplast generation and transformation in Bacillus thuringiensis subsp. kurstaki. J Bacteriol. 1987 Dec;169(12):5423–5428. [PMC free article] [PubMed]
  • Donovan WP, Dankocsik CC, Gilbert MP, Gawron-Burke MC, Groat RG, Carlton BC. Amino acid sequence and entomocidal activity of the P2 crystal protein. An insect toxin from Bacillus thuringiensis var. kurstaki. J Biol Chem. 1988 Jan 5;263(1):561–567. [PubMed]
  • Donovan WP, Gonzalez JM, Jr, Gilbert MP, Dankocsik C. Isolation and characterization of EG2158, a new strain of Bacillus thuringiensis toxic to coleopteran larvae, and nucleotide sequence of the toxin gene. Mol Gen Genet. 1988 Nov;214(3):365–372. [PubMed]
  • Dubnau D, Davidoff-Abelson R, Scher B, Cirigliano C. Fate of transforming deoxyribonucleic acid after uptake by competent Bacillus subtilis: phenotypic characterization of radiation-sensitive recombination-deficient mutants. J Bacteriol. 1973 Apr;114(1):273–286. [PMC free article] [PubMed]
  • González JM, Jr, Carlton BC. A large transmissible plasmid is required for crystal toxin production in Bacillus thuringiensis variety israelensis. Plasmid. 1984 Jan;11(1):28–38. [PubMed]
  • Heierson A, Landén R, Lövgren A, Dalhammar G, Boman HG. Transformation of vegetative cells of Bacillus thuringiensis by plasmid DNA. J Bacteriol. 1987 Mar;169(3):1147–1152. [PMC free article] [PubMed]
  • Höfte H, Whiteley HR. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol Rev. 1989 Jun;53(2):242–255. [PMC free article] [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Lereclus D, Arantès O, Chaufaux J, Lecadet M. Transformation and expression of a cloned delta-endotoxin gene in Bacillus thuringiensis. FEMS Microbiol Lett. 1989 Jul 15;51(1):211–217. [PubMed]
  • Li E, Yousten AA. Metalloprotease from Bacillus thuringiensis. Appl Microbiol. 1975 Sep;30(3):354–361. [PMC free article] [PubMed]
  • MacNeil DJ. Characterization of a unique methyl-specific restriction system in Streptomyces avermitilis. J Bacteriol. 1988 Dec;170(12):5607–5612. [PMC free article] [PubMed]
  • Marinus MG. Location of DNA methylation genes on the Escherichia coli K-12 genetic map. Mol Gen Genet. 1973 Dec 14;127(1):47–55. [PubMed]
  • Marinus MG, Carraway M, Frey AZ, Brown L, Arraj JA. Insertion mutations in the dam gene of Escherichia coli K-12. Mol Gen Genet. 1983;192(1-2):288–289. [PubMed]
  • Mettus AM, Macaluso A. Expression of Bacillus thuringiensis delta-endotoxin genes during vegetative growth. Appl Environ Microbiol. 1990 Apr;56(4):1128–1134. [PMC free article] [PubMed]
  • Norton NB, Orzech KA, Burke WF., Jr Construction and characterization of plasmid vectors for cloning in the entomocidal organism Bacillus sphaericus 1593. Plasmid. 1985 May;13(3):211–214. [PubMed]
  • Schurter W, Geiser M, Mathé D. Efficient transformation of Bacillus thuringiensis and B. cereus via electroporation: transformation of acrystalliferous strains with a cloned delta-endotoxin gene. Mol Gen Genet. 1989 Jul;218(1):177–181. [PubMed]
  • Shigekawa K, Dower WJ. Electroporation of eukaryotes and prokaryotes: a general approach to the introduction of macromolecules into cells. Biotechniques. 1988 Sep;6(8):742–751. [PubMed]
  • Streicher S, Gurney E, Valentine RC. Transduction of the nitrogen-fixation genes in Klebsiella pneumoniae. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1174–1177. [PMC free article] [PubMed]
  • Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. [PMC free article] [PubMed]
  • Tung AS, Ju ST, Sato S, Nisonoff A. Production of large amounts of antibodies in individual mice. J Immunol. 1976 Mar;116(3):676–681. [PubMed]
  • Von Tersch MA, Carlton BC. Molecular cloning of structural and immunity genes for megacins A-216 and A-19213 in Bacillus megaterium. J Bacteriol. 1984 Dec;160(3):854–859. [PMC free article] [PubMed]

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