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J Bacteriol. Oct 1993; 175(20): 6637–6651.
PMCID: PMC206775

Construction of an SfiI macrorestriction map of the Candida albicans genome.

Abstract

The opportunistic fungal pathogen, Candida albicans, is diploid as usually isolated and has no apparent sexual cycle. Genetic analysis has therefore been very difficult. Molecular genetics has yielded important information in the past few years, but it too is hampered by the lack of a good genetic map. Using the well-characterized strain 1006 and strain WO-1, which undergoes the white-opaque phenotypic transition, we have developed a genomic restriction map of C. albicans with the enzyme SfiI. There are approximately 34 SfiI restriction sites in the C. albicans genome. Restriction fragments were separated by pulsed-field electrophoresis and were assigned to chromosomes by hybridization of complete and partial digests with known chromosome-specific probes as well as by digestion of isolated chromosomes. Telomeric fragments were identified by hybridization with a telomere-specific probe (C. Sadhu, M.J. McEachern, E.P. Rustchenko-Bulgac, J. Schmid, D.R. Soll, and J.B. Hicks, J. Bacteriol. 173:842-850, 1991). WO-1 differs from 1006 in that it has undergone three reciprocal chromosomal translocations. Analysis of the translocation products indicates that each translocation has occurred at or near an SfiI site; thus, the SfiI fragments from the two strains are similar or identical. The tendency for translocation to occur at or near SfiI sites may be related to the repeated sequence RPS 1, which contains four such sites and could provide homology for ectopic pairing and crossing over. The genome size of both strains is about 16 to 17 megabases, in good agreement with previous determinations.

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  • Altboum Z, Gottlieb S, Lebens GA, Polacheck I, Segal E. Isolation of the Candida albicans histidinol dehydrogenase (HIS4) gene and characterization of a histidine auxotroph. J Bacteriol. 1990 Jul;172(7):3898–3904. [PMC free article] [PubMed]
  • Anderson J, Cundiff L, Schnars B, Gao MX, Mackenzie I, Soll DR. Hypha formation in the white-opaque transition of Candida albicans. Infect Immun. 1989 Feb;57(2):458–467. [PMC free article] [PubMed]
  • Anderson J, Mihalik R, Soll DR. Ultrastructure and antigenicity of the unique cell wall pimple of the Candida opaque phenotype. J Bacteriol. 1990 Jan;172(1):224–235. [PMC free article] [PubMed]
  • Anderson JM, Soll DR. Unique phenotype of opaque cells in the white-opaque transition of Candida albicans. J Bacteriol. 1987 Dec;169(12):5579–5588. [PMC free article] [PubMed]
  • Baccanari DP, Tansik RL, Joyner SS, Fling ME, Smith PL, Freisheim JH. Characterization of Candida albicans dihydrofolate reductase. J Biol Chem. 1989 Jan 15;264(2):1100–1107. [PubMed]
  • Bishop RP, Miles MA. Chromosome size polymorphisms of Leishmania donovani. Mol Biochem Parasitol. 1987 Jul;24(3):263–272. [PubMed]
  • Brody H, Carbon J. Electrophoretic karyotype of Aspergillus nidulans. Proc Natl Acad Sci U S A. 1989 Aug;86(16):6260–6263. [PMC free article] [PubMed]
  • Carle GF, Olson MV. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis. Nucleic Acids Res. 1984 Jul 25;12(14):5647–5664. [PMC free article] [PubMed]
  • Carle GF, Olson MV. An electrophoretic karyotype for yeast. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3756–3760. [PMC free article] [PubMed]
  • Chu WS, Rikkerink EH, Magee PT. Genetics of the white-opaque transition in Candida albicans: demonstration of switching recessivity and mapping of switching genes. J Bacteriol. 1992 May;174(9):2951–2957. [PMC free article] [PubMed]
  • Corcoran LM, Thompson JK, Walliker D, Kemp DJ. Homologous recombination within subtelomeric repeat sequences generates chromosome size polymorphisms in P. falciparum. Cell. 1988 Jun 3;53(5):807–813. [PubMed]
  • Engman DM, Reddy LV, Donelson JE, Kirchhoff LV. Trypanosoma cruzi exhibits inter- and intra-strain heterogeneity in molecular karyotype and chromosomal gene location. Mol Biochem Parasitol. 1987 Jan 15;22(2-3):115–123. [PubMed]
  • Fan JB, Chikashige Y, Smith CL, Niwa O, Yanagida M, Cantor CR. Construction of a Not I restriction map of the fission yeast Schizosaccharomyces pombe genome. Nucleic Acids Res. 1989 Apr 11;17(7):2801–2818. [PMC free article] [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Gillum AM, Tsay EY, Kirsch DR. Isolation of the Candida albicans gene for orotidine-5'-phosphate decarboxylase by complementation of S. cerevisiae ura3 and E. coli pyrF mutations. Mol Gen Genet. 1984;198(1):179–182. [PubMed]
  • Goshorn AK, Scherer S. Genetic analysis of prototrophic natural variants of Candida albicans. Genetics. 1989 Dec;123(4):667–673. [PMC free article] [PubMed]
  • Hube B, Turver CJ, Odds FC, Eiffert H, Boulnois GJ, Köchel H, Rüchel R. Sequence of the Candida albicans gene encoding the secretory aspartate proteinase. J Med Vet Mycol. 1991;29(2):129–132. [PubMed]
  • Iwaguchi S, Homma M, Tanaka K. Variation in the electrophoretic karyotype analysed by the assignment of DNA probes in Candida albicans. J Gen Microbiol. 1990 Dec;136(12):2433–2442. [PubMed]
  • Iwaguchi S, Homma M, Chibana H, Tanaka K. Isolation and characterization of a repeated sequence (RPS1) of Candida albicans. J Gen Microbiol. 1992 Sep;138(9):1893–1900. [PubMed]
  • Iwaguchi S, Homma M, Tanaka K. Clonal variation of chromosome size derived from the rDNA cluster region in Candida albicans. J Gen Microbiol. 1992 Jun;138(6):1177–1184. [PubMed]
  • Kakar SN, Partridge RM, Magee PT. A genetic analysis of Candida albicans: isolation of a wide variety of auxotrophs and demonstration of linkage and complementation. Genetics. 1983 Jun;104(2):241–255. [PMC free article] [PubMed]
  • Kinscherf TG, Leong SA. Molecular analysis of the karyotype of Ustilago maydis. Chromosoma. 1988;96(6):427–433. [PubMed]
  • Kurtz MB, Cortelyou MW, Kirsch DR. Integrative transformation of Candida albicans, using a cloned Candida ADE2 gene. Mol Cell Biol. 1986 Jan;6(1):142–149. [PMC free article] [PubMed]
  • Kurtz MB, Cortelyou MW, Miller SM, Lai M, Kirsch DR. Development of autonomously replicating plasmids for Candida albicans. Mol Cell Biol. 1987 Jan;7(1):209–217. [PMC free article] [PubMed]
  • Lasker BA, Carle GF, Kobayashi GS, Medoff G. Comparison of the separation of Candida albicans chromosome-sized DNA by pulsed-field gel electrophoresis techniques. Nucleic Acids Res. 1989 May 25;17(10):3783–3793. [PMC free article] [PubMed]
  • Le Blancq SM, Korman SH, Van der Ploeg LH. Frequent rearrangements of rRNA-encoding chromosomes in Giardia lamblia. Nucleic Acids Res. 1991 Aug 25;19(16):4405–4412. [PMC free article] [PubMed]
  • Magee BB, D'Souza TM, Magee PT. Strain and species identification by restriction fragment length polymorphisms in the ribosomal DNA repeat of Candida species. J Bacteriol. 1987 Apr;169(4):1639–1643. [PMC free article] [PubMed]
  • Magee BB, Koltin Y, Gorman JA, Magee PT. Assignment of cloned genes to the seven electrophoretically separated Candida albicans chromosomes. Mol Cell Biol. 1988 Nov;8(11):4721–4726. [PMC free article] [PubMed]
  • Magee BB, Magee PT. Electrophoretic karyotypes and chromosome numbers in Candida species. J Gen Microbiol. 1987 Feb;133(2):425–430. [PubMed]
  • Magee PT, Bowdin L, Staudinger J. Comparison of molecular typing methods for Candida albicans. J Clin Microbiol. 1992 Oct;30(10):2674–2679. [PMC free article] [PubMed]
  • Mason MM, Lasker BA, Riggsby WS. Molecular probe for identification of medically important Candida species and Torulopsis glabrata. J Clin Microbiol. 1987 Mar;25(3):563–566. [PMC free article] [PubMed]
  • Meinkoth J, Wahl G. Hybridization of nucleic acids immobilized on solid supports. Anal Biochem. 1984 May 1;138(2):267–284. [PubMed]
  • Merz WG, Connelly C, Hieter P. Variation of electrophoretic karyotypes among clinical isolates of Candida albicans. J Clin Microbiol. 1988 May;26(5):842–845. [PMC free article] [PubMed]
  • Olaiya AF, Sogin SJ. Ploidy determination of Canadida albicans. J Bacteriol. 1979 Dec;140(3):1043–1049. [PMC free article] [PubMed]
  • Pologe LG, Ravetch JV. Large deletions result from breakage and healing of P. falciparum chromosomes. Cell. 1988 Dec 2;55(5):869–874. [PubMed]
  • Riggsby WS, Torres-Bauza LJ, Wills JW, Townes TM. DNA content, kinetic complexity, and the ploidy question in Candida albicans. Mol Cell Biol. 1982 Jul;2(7):853–862. [PMC free article] [PubMed]
  • Rikkerink EH, Magee BB, Magee PT. Opaque-white phenotype transition: a programmed morphological transition in Candida albicans. J Bacteriol. 1988 Feb;170(2):895–899. [PMC free article] [PubMed]
  • Rosenbluh A, Mevarech M, Koltin Y, Gorman JA. Isolation of genes from Candida albicans by complementation in Saccharomyces cerevisiae. Mol Gen Genet. 1985;200(3):500–502. [PubMed]
  • Rustchenko-Bulgac EP. Variations of Candida albicans electrophoretic karyotypes. J Bacteriol. 1991 Oct;173(20):6586–6596. [PMC free article] [PubMed]
  • Rustchenko-Bulgac EP, Sherman F, Hicks JB. Chromosomal rearrangements associated with morphological mutants provide a means for genetic variation of Candida albicans. J Bacteriol. 1990 Mar;172(3):1276–1283. [PMC free article] [PubMed]
  • Sadhu C, McEachern MJ, Rustchenko-Bulgac EP, Schmid J, Soll DR, Hicks JB. Telomeric and dispersed repeat sequences in Candida yeasts and their use in strain identification. J Bacteriol. 1991 Jan;173(2):842–850. [PMC free article] [PubMed]
  • Scherer S, Magee PT. Genetics of Candida albicans. Microbiol Rev. 1990 Sep;54(3):226–241. [PMC free article] [PubMed]
  • Schwartz DC, Cantor CR. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. [PubMed]
  • Shepherd MG, Poulter RT, Sullivan PA. Candida albicans: biology, genetics, and pathogenicity. Annu Rev Microbiol. 1985;39:579–614. [PubMed]
  • Singer SC, Richards CA, Ferone R, Benedict D, Ray P. Cloning, purification, and properties of Candida albicans thymidylate synthase. J Bacteriol. 1989 Mar;171(3):1372–1378. [PMC free article] [PubMed]
  • Slutsky B, Buffo J, Soll DR. High-frequency switching of colony morphology in Candida albicans. Science. 1985 Nov 8;230(4726):666–669. [PubMed]
  • Slutsky B, Staebell M, Anderson J, Risen L, Pfaller M, Soll DR. "White-opaque transition": a second high-frequency switching system in Candida albicans. J Bacteriol. 1987 Jan;169(1):189–197. [PMC free article] [PubMed]
  • Smith HA, Allaudeen HS, Whitman MH, Koltin Y, Gorman JA. Isolation and characterization of a beta-tubulin gene from Candida albicans. Gene. 1988;63(1):53–63. [PubMed]
  • Snell RG, Hermans IF, Wilkins RJ, Corner BE. Chromosomal variations in Candida albicans. Nucleic Acids Res. 1987 Apr 24;15(8):3625–3625. [PMC free article] [PubMed]
  • Suzuki T, Kobayashi I, Kanbe T, Tanaka K. High frequency variation of colony morphology and chromosome reorganization in the pathogenic yeast Candida albicans. J Gen Microbiol. 1989 Feb;135(Pt 2):425–434. [PubMed]
  • Thrash-Bingham C, Gorman JA. DNA translocations contribute to chromosome length polymorphisms in Candida albicans. Curr Genet. 1992 Aug;22(2):93–100. [PubMed]
  • Vollrath D, Davis RW, Connelly C, Hieter P. Physical mapping of large DNA by chromosome fragmentation. Proc Natl Acad Sci U S A. 1988 Aug;85(16):6027–6031. [PMC free article] [PubMed]
  • Walz M, Kück U. Polymorphic karyotypes in related Acremonium strains. Curr Genet. 1991 Feb;19(2):73–76. [PubMed]
  • Whelan WL, Partridge RM, Magee PT. Heterozygosity and segregation in Candida albicans. Mol Gen Genet. 1980;180(1):107–113. [PubMed]
  • Whelan WL, Soll DR. Mitotic recombination in Candida albicans: recessive lethal alleles linked to a gene required for methionine biosynthesis. Mol Gen Genet. 1982;187(3):477–485. [PubMed]
  • Wickes B, Staudinger J, Magee BB, Kwon-Chung KJ, Magee PT, Scherer S. Physical and genetic mapping of Candida albicans: several genes previously assigned to chromosome 1 map to chromosome R, the rDNA-containing linkage group. Infect Immun. 1991 Jul;59(7):2480–2484. [PMC free article] [PubMed]
  • Wilkie AO, Higgs DR, Rack KA, Buckle VJ, Spurr NK, Fischel-Ghodsian N, Ceccherini I, Brown WR, Harris PC. Stable length polymorphism of up to 260 kb at the tip of the short arm of human chromosome 16. Cell. 1991 Feb 8;64(3):595–606. [PubMed]
  • Zakian VA, Blanton HM. Distribution of telomere-associated sequences on natural chromosomes in Saccharomyces cerevisiae. Mol Cell Biol. 1988 May;8(5):2257–2260. [PMC free article] [PubMed]

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