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J Bacteriol. Oct 1993; 175(19): 6212–6219.
PMCID: PMC206716

Characterization of the ferrous iron uptake system of Escherichia coli.

Abstract

Escherichia coli has an iron(II) transport system (feo) which may make an important contribution to the iron supply of the cell under anaerobic conditions. Cloning and sequencing of the iron(II) transport genes revealed an open reading frame (feoA) possibly coding for a small protein with 75 amino acids and a membrane protein with 773 amino acids (feoB). The upstream region of feoAB contained a binding site for the regulatory protein Fur, which acts with iron(II) as a corepressor in all known iron transport systems of E. coli. In addition, a Fnr binding site was identified in the promoter region. The FeoB protein had an apparent molecular mass of 70 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and was localized in the cytoplasmic membrane. The sequence revealed regions of homology to ATPases, which indicates that ferrous iron uptake may be ATP driven. FeoA or FeoB mutants could be complemented by clones with the feoA or feoB gene, respectively.

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Selected References

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  • Angerer A, Klupp B, Braun V. Iron transport systems of Serratia marcescens. J Bacteriol. 1992 Feb;174(4):1378–1387. [PMC free article] [PubMed]
  • Berish SA, Chen CY, Mietzner TA, Morse SA. Expression of a functional neisserial fbp gene in Escherichia coli. Mol Microbiol. 1992 Sep;6(18):2607–2615. [PubMed]
  • Bezkorovainy A, Solberg L, Poch M, Miller-Catchpole R. Ferrous iron uptake by Bifidobacterium bifidum var. pennsylvanicus: the effect of metals and metabolic inhibitors. Int J Biochem. 1987;19(6):517–522. [PubMed]
  • Burkhardt R, Braun V. Nucleotide sequence of the fhuC and fhuD genes involved in iron (III) hydroxamate transport: domains in FhuC homologous to ATP-binding proteins. Mol Gen Genet. 1987 Aug;209(1):49–55. [PubMed]
  • Cornelissen CN, Biswas GD, Tsai J, Paruchuri DK, Thompson SA, Sparling PF. Gonococcal transferrin-binding protein 1 is required for transferrin utilization and is homologous to TonB-dependent outer membrane receptors. J Bacteriol. 1992 Sep;174(18):5788–5797. [PMC free article] [PubMed]
  • Dancis A, Roman DG, Anderson GJ, Hinnebusch AG, Klausner RD. Ferric reductase of Saccharomyces cerevisiae: molecular characterization, role in iron uptake, and transcriptional control by iron. Proc Natl Acad Sci U S A. 1992 May 1;89(9):3869–3873. [PMC free article] [PubMed]
  • de Lorenzo V, Herrero M, Jakubzik U, Timmis KN. Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. J Bacteriol. 1990 Nov;172(11):6568–6572. [PMC free article] [PubMed]
  • Eiglmeier K, Honoré N, Iuchi S, Lin EC, Cole ST. Molecular genetic analysis of FNR-dependent promoters. Mol Microbiol. 1989 Jul;3(7):869–878. [PubMed]
  • Evans SL, Arceneaux JE, Byers BR, Martin ME, Aranha H. Ferrous iron transport in Streptococcus mutans. J Bacteriol. 1986 Dec;168(3):1096–1099. [PMC free article] [PubMed]
  • Hancock RE, Hantke K, Braun V. Iron transport in Escherichia coli K-12. 2,3-Dihydroxybenzoate-promoted iron uptake. Arch Microbiol. 1977 Sep 28;114(3):231–239. [PubMed]
  • Hussein S, Hantke K, Braun V. Citrate-dependent iron transport system in Escherichia coli K-12. Eur J Biochem. 1981 Jul;117(2):431–437. [PubMed]
  • Johnson W, Varner L, Poch M. Acquisition of iron by Legionella pneumophila: role of iron reductase. Infect Immun. 1991 Jul;59(7):2376–2381. [PMC free article] [PubMed]
  • Klein P, Kanehisa M, DeLisi C. The detection and classification of membrane-spanning proteins. Biochim Biophys Acta. 1985 May 28;815(3):468–476. [PubMed]
  • Kohara Y, Akiyama K, Isono K. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell. 1987 Jul 31;50(3):495–508. [PubMed]
  • Miller VL, Mekalanos JJ. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J Bacteriol. 1988 Jun;170(6):2575–2583. [PMC free article] [PubMed]
  • Mimura CS, Holbrook SR, Ames GF. Structural model of the nucleotide-binding conserved component of periplasmic permeases. Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):84–88. [PMC free article] [PubMed]
  • Nassif X, Mazert MC, Mounier J, Sansonetti PJ. Evaluation with an iuc::Tn10 mutant of the role of aerobactin production in the virulence of Shigella flexneri. Infect Immun. 1987 Sep;55(9):1963–1969. [PMC free article] [PubMed]
  • Niehaus F, Hantke K, Unden G. Iron content and FNR-dependent gene regulation in Escherichia coli. FEMS Microbiol Lett. 1991 Dec 1;68(3):319–323. [PubMed]
  • Nucifora G, Chu L, Misra TK, Silver S. Cadmium resistance from Staphylococcus aureus plasmid pI258 cadA gene results from a cadmium-efflux ATPase. Proc Natl Acad Sci U S A. 1989 May;86(10):3544–3548. [PMC free article] [PubMed]
  • O'Regan M, Gloeckler R, Bernard S, Ledoux C, Ohsawa I, Lemoine Y. Nucleotide sequence of the bioH gene of Escherichia coli. Nucleic Acids Res. 1989 Oct 11;17(19):8004–8004. [PMC free article] [PubMed]
  • Payne SM. Iron and virulence in Shigella. Mol Microbiol. 1989 Sep;3(9):1301–1306. [PubMed]
  • Silver S, Nucifora G, Chu L, Misra TK. Bacterial resistance ATPases: primary pumps for exporting toxic cations and anions. Trends Biochem Sci. 1989 Feb;14(2):76–80. [PubMed]
  • Staudenmaier H, Van Hove B, Yaraghi Z, Braun V. Nucleotide sequences of the fecBCDE genes and locations of the proteins suggest a periplasmic-binding-protein-dependent transport mechanism for iron(III) dicitrate in Escherichia coli. J Bacteriol. 1989 May;171(5):2626–2633. [PMC free article] [PubMed]
  • Stewart V. Requirement of Fnr and NarL functions for nitrate reductase expression in Escherichia coli K-12. J Bacteriol. 1982 Sep;151(3):1320–1325. [PMC free article] [PubMed]
  • Stewart V, MacGregor CH. Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE, and chlG loci. J Bacteriol. 1982 Aug;151(2):788–799. [PMC free article] [PubMed]
  • Stojiljkovic I, Cobeljic M, Hantke K. Escherichia coli K-12 ferrous iron uptake mutants are impaired in their ability to colonize the mouse intestine. FEMS Microbiol Lett. 1993 Mar 15;108(1):111–115. [PubMed]
  • Stojiljković I, Cobeljić M, Trgovcević Z, Salaj-Smic E. The ability of rifampin-resistant Escherichia coli to colonize the mouse intestine is enhanced by the presence of a plasmid-encoded aerobactin-iron(III) uptake system. FEMS Microbiol Lett. 1991 Dec 15;69(1):89–93. [PubMed]
  • Stojiljkovic I, Hantke K. Hemin uptake system of Yersinia enterocolitica: similarities with other TonB-dependent systems in gram-negative bacteria. EMBO J. 1992 Dec;11(12):4359–4367. [PMC free article] [PubMed]
  • Tabor S, Richardson CC. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074–1078. [PMC free article] [PubMed]
  • Takeshita S, Sato M, Toba M, Masahashi W, Hashimoto-Gotoh T. High-copy-number and low-copy-number plasmid vectors for lacZ alpha-complementation and chloramphenicol- or kanamycin-resistance selection. Gene. 1987;61(1):63–74. [PubMed]
  • Walker JE, Saraste M, Runswick MJ, Gay NJ. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1982;1(8):945–951. [PMC free article] [PubMed]
  • Wang RF, Kushner SR. Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli. Gene. 1991 Apr;100:195–199. [PubMed]

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