• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Sep 1993; 175(17): 5359–5365.
PMCID: PMC206590

Molecular analyses of the Salmonella g. . . flagellar antigen complex.


Salmonella flagellar filaments are polymers of a highly antigenic protein, termed flagellin. Eight main subfactors have been identified in the Salmonella phase-1 g. . . series flagellar antigen. To determine the molecular basis for expression of the epitopes by which the g. . . family subfactors are distinguished, 10 members of this series were selected and their fliC (the structural gene for phase-1 flagellin) genes were sequenced. Comparative analyses of the inferred primary structures of these flagellins did not allow the identification of linear epitopes responsible for the antigen subfactors. This suggests that conformational aspects are involved in determining the antigenic specificity in these cases. A phylogenetic analysis of the flagellin sequences showed that members of the g. . . series do not form a single coherent unit.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Brey RN, Bixler GS, Fulginiti JP, Dilts DA, Sabara MI. Oral delivery of antigens in live bacterial vectors. Adv Exp Med Biol. 1991;303:169–184. [PubMed]
  • DePamphilis ML, Adler J. Purification of intact flagella from Escherichia coli and Bacillus subtilis. J Bacteriol. 1971 Jan;105(1):376–383. [PMC free article] [PubMed]
  • Gray GS, Fitch WM. Evolution of antibiotic resistance genes: the DNA sequence of a kanamycin resistance gene from Staphylococcus aureus. Mol Biol Evol. 1983 Dec;1(1):57–66. [PubMed]
  • Iino T. Genetics of structure and function of bacterial flagella. Annu Rev Genet. 1977;11:161–182. [PubMed]
  • Iino T, Komeda Y, Kutsukake K, Macnab RM, Matsumura P, Parkinson JS, Simon MI, Yamaguchi S. New unified nomenclature for the flagellar genes of Escherichia coli and Salmonella typhimurium. Microbiol Rev. 1988 Dec;52(4):533–535. [PMC free article] [PubMed]
  • Joys TM. The covalent structure of the phase-1 flagellar filament protein of Salmonella typhimurium and its comparison with other flagellins. J Biol Chem. 1985 Dec 15;260(29):15758–15761. [PubMed]
  • Joys TM, Martin JF. Identification of amino acid changes in serological mutants of the i flagellar antigen of Salmonella typhimurium. Microbios. 1973;7(25):71–73. [PubMed]
  • Joys TM, Schödel F. Epitope mapping of the d flagellar antigen of Salmonella muenchen. Infect Immun. 1991 Sep;59(9):3330–3332. [PMC free article] [PubMed]
  • Joys TM, Stocker BA. Recombination in H1, the gene determining the flagellar antigen-i of Salmonella typhimurium; mapping of H1 and fla mutations. J Gen Microbiol. 1969 Oct;58(2):267–275. [PubMed]
  • Kuwajima G, Asaka J, Fujiwara T, Fujiwara T, Node K, Kondo E. Nucleotide sequence of the hag gene encoding flagellin of Escherichia coli. J Bacteriol. 1986 Dec;168(3):1479–1483. [PMC free article] [PubMed]
  • Lipman DJ, Pearson WR. Rapid and sensitive protein similarity searches. Science. 1985 Mar 22;227(4693):1435–1441. [PubMed]
  • McDonough MW, Smith SE. Molecular weight variation among bacterial flagellins. Microbios. 1976;16(63):49–53. [PubMed]
  • McEwen J, Levi R, Horwitz RJ, Arnon R. Synthetic recombinant vaccine expressing influenza haemagglutinin epitope in Salmonella flagellin leads to partial protection in mice. Vaccine. 1992;10(6):405–411. [PubMed]
  • Mount DW, Conrad B. Microcomputer programs for graphic analysis of nucleic acid and protein sequences. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):811–817. [PMC free article] [PubMed]
  • Namba K, Yamashita I, Vonderviszt F. Structure of the core and central channel of bacterial flagella. Nature. 1989 Dec 7;342(6250):648–654. [PubMed]
  • Newton SM, Jacob CO, Stocker BA. Immune response to cholera toxin epitope inserted in Salmonella flagellin. Science. 1989 Apr 7;244(4900):70–72. [PubMed]
  • Newton SM, Kotb M, Poirier TP, Stocker BA, Beachey EH. Expression and immunogenicity of a streptococcal M protein epitope inserted in Salmonella flagellin. Infect Immun. 1991 Jun;59(6):2158–2165. [PMC free article] [PubMed]
  • Parish CR, Wistar R, Ada GL. Cleavage of bacterial flagellin with cyanogen bromide. Antigenic properties of the protein fragments. Biochem J. 1969 Jul;113(3):501–506. [PMC free article] [PubMed]
  • Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. [PubMed]
  • Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350–1354. [PubMed]
  • Selander RK, Smith NH, Li J, Beltran P, Ferris KE, Kopecko DJ, Rubin FA. Molecular evolutionary genetics of the cattle-adapted serovar Salmonella dublin. J Bacteriol. 1992 Jun;174(11):3587–3592. [PMC free article] [PubMed]
  • Smith NH, Beltran P, Selander RK. Recombination of Salmonella phase 1 flagellin genes generates new serovars. J Bacteriol. 1990 May;172(5):2209–2216. [PMC free article] [PubMed]
  • Smith NH, Selander RK. Sequence invariance of the antigen-coding central region of the phase 1 flagellar filament gene (fliC) among strains of Salmonella typhimurium. J Bacteriol. 1990 Feb;172(2):603–609. [PMC free article] [PubMed]
  • Stocker BA. Aromatic-dependent Salmonella as live vaccine presenters of foreign epitopes as inserts in flagellin. Res Microbiol. 1990 Sep-Oct;141(7-8):787–796. [PubMed]
  • Szekely E, Simon M. DNA sequence adjacent to flagellar genes and evolution of flagellar-phase variation. J Bacteriol. 1983 Jul;155(1):74–81. [PMC free article] [PubMed]
  • Wei LN, Joys TM. Covalent structure of three phase-1 flagellar filament proteins of Salmonella. J Mol Biol. 1985 Dec 20;186(4):791–803. [PubMed]
  • Wei LN, Joys TM. The nucleotide sequence of the H-1r gene of Salmonella rubislaw. Nucleic Acids Res. 1986 Oct 24;14(20):8227–8227. [PMC free article] [PubMed]
  • Wu JY, Newton S, Judd A, Stocker B, Robinson WS. Expression of immunogenic epitopes of hepatitis B surface antigen with hybrid flagellin proteins by a vaccine strain of Salmonella. Proc Natl Acad Sci U S A. 1989 Jun;86(12):4726–4730. [PMC free article] [PubMed]
  • Yamaguchi S, Iino T. Genetic determination of the antigenic specificity of flagellar protein in salmonella. J Gen Microbiol. 1969 Jan;55(1):59–74. [PubMed]
  • Zieg J, Silverman M, Hilmen M, Simon M. Recombinational switch for gene expression. Science. 1977 Apr 8;196(4286):170–172. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...