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Figure 5.

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RNA foci in DM1 contain spliced DMPK RNA. (A) In HSMM myoblast nuclei, DMPK intron 9, which was detected with a PCR-generated probe (green),detects an RNA focus that overlaps the focus of RNA detected with the DMPK cDNA probe (red). The right panel shows the three-color merged image. (B) In DM1 differentiated muscle cells, signals seen with the DMPK intron 9 probe (green) do not overlap DM1 RNA foci detected with MBNL1 (red). MBNL1 overlap with DMPK RNA is seen in Fig. 6 B. The right panel is a merged three-color image. Bar, 5 μm.

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