• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Aug 1992; 174(15): 4952–4959.
PMCID: PMC206308

Isolation and characterization of a Tn551-autolysis mutant of Staphylococcus aureus.

Abstract

A Lyt- mutant with reduced autolytic activity was isolated after Tn551 mutagenesis of the methicillin-susceptible Staphylococcus aureus laboratory strain RN450. The Lyt- phenotype could be transferred back into the parent and into a variety of other S. aureus strains by transduction of the transposon marker. Southern analysis has located the Tn551 insert to a 3.2-kb HindIII DNA fragment on the SmaI B fragment of the staphylococcal chromosome. The Lyt- phenotype included reduced rates of cell wall turnover and autolysis induced by detergent or methicillin treatment; however, the rate of methicillin-induced killing was not affected. Peptidoglycans prepared from the parental and mutant cells showed identical muropeptide compositions, as resolved by a high-resolution high-pressure liquid chromatography technique. On the other hand, LiCl extracts of the mutant cells contained reduced amounts of total protein and lower specific cell wall-degrading activity compared with those of extracts of parental cells. The profile of bacteriolytic enzymes as detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed multiple band differences between mutant and parental cells; a major lytic band with properties characteristic of the staphylococcal endo-beta-N-acetylglucosaminidase was completely absent from the Lyt- cells. The Lyt- phenotype transduced into a series of methicillin-resistant strains of both homogeneous and heterogeneous phenotypes caused only a modest decrease in the level of methicillin resistance, as determined by population analysis.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.6M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. [PubMed]
  • Chatterjee AN, Wong W, Young FE, Gilpin RW. Isolation and characterization of a mutant of Staphylococcus aureus deficient in autolytic activity. J Bacteriol. 1976 Mar;125(3):961–967. [PMC free article] [PubMed]
  • de Jonge BL, de Lencastre H, Tomasz A. Suppression of autolysis and cell wall turnover in heterogeneous Tn551 mutants of a methicillin-resistant Staphylococcus aureus strain. J Bacteriol. 1991 Feb;173(3):1105–1110. [PMC free article] [PubMed]
  • Huff E, Silverman CS, Adams NJ, Awkard WS. Extracellular cell wall lytic enzyme from Staphylococcus aureus: purification and partial characterization. J Bacteriol. 1970 Sep;103(3):761–769. [PMC free article] [PubMed]
  • Jayaswal RK, Lee YI, Wilkinson BJ. Cloning and expression of a Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity. J Bacteriol. 1990 Oct;172(10):5783–5788. [PMC free article] [PubMed]
  • Koyama T, Yamada M, Matsuhashi M. Formation of regular packets of Staphylococcus aureus cells. J Bacteriol. 1977 Mar;129(3):1518–1523. [PMC free article] [PubMed]
  • Leclerc D, Asselin A. Detection of bacterial cell wall hydrolases after denaturing polyacrylamide gel electrophoresis. Can J Microbiol. 1989 Aug;35(8):749–753. [PubMed]
  • Maidhof H, Reinicke B, Blümel P, Berger-Bächi B, Labischinski H. femA, which encodes a factor essential for expression of methicillin resistance, affects glycine content of peptidoglycan in methicillin-resistant and methicillin-susceptible Staphylococcus aureus strains. J Bacteriol. 1991 Jun;173(11):3507–3513. [PMC free article] [PubMed]
  • Murakami K, Tomasz A. Involvement of multiple genetic determinants in high-level methicillin resistance in Staphylococcus aureus. J Bacteriol. 1989 Feb;171(2):874–879. [PMC free article] [PubMed]
  • PARK JT, JOHNSON MJ. A submicrodetermination of glucose. J Biol Chem. 1949 Nov;181(1):149–151. [PubMed]
  • Singer HJ, Wise EM, Jr, Park JT. Properties and purification of N-acetylmuramyl-L-alanine amidase from Staphylococcus aureus H. J Bacteriol. 1972 Nov;112(2):932–939. [PMC free article] [PubMed]
  • Sugai M, Akiyama T, Komatsuzawa H, Miyake Y, Suginaka H. Characterization of sodium dodecyl sulfate-stable Staphylococcus aureus bacteriolytic enzymes by polyacrylamide gel electrophoresis. J Bacteriol. 1990 Nov;172(11):6494–6498. [PMC free article] [PubMed]
  • Sugai M, Koike H, Hong YM, Miyake Y, Nogami R, Suginaka H. Purification of a 51 kDa endo-beta-N-acetylglucosaminidase from Staphylococcus aureus. FEMS Microbiol Lett. 1989 Oct 15;52(3):267–272. [PubMed]
  • Tipper DJ. Mechanism of autolysis of isolated cell walls of Staphylococcus aureus. J Bacteriol. 1969 Feb;97(2):837–847. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...