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J Bacteriol. Jul 1992; 174(14): 4769–4776.
PMCID: PMC206274

Regulation of katF and katE in Escherichia coli K-12 by weak acids.


Chromosomal transcriptional and translational lacZ fusions to the katE (structural gene for the HPII hydroperoxidase) and katF (putative sigma factor required for katE expression) genes of Escherichia coli were isolated, and the regulation of these fusions was used to identify factors that control the expression of these two important antioxidant factors. While katE was found to be regulated primarily at the level of transcription (since induction patterns were similar for both transcriptional and translational fusions), katF expression was a function of both transcriptional and translational signals. The katE gene was induced 57-fold as cells entered the stationary phase, while katF was induced 23-fold. katF induction was coincident with katE induction and occurred at the onset of the stationary growth phase. Expression of both katE and katF could be induced by resuspending uninduced exponential-phase cells in spent culture supernatant recovered from stationary-phase cells. The component of stationary-phase culture supernatant responsible for induction of the katF regulon appeared to be acetate, since expression of both katE and katF fusions was induced when exponential-phase cells were exposed to this weak acid. Other weak acids, including propionate and benzoate, were also found to be effective inducers of expression of both katF and katE. Induction of katE and katF fusions was unaffected in merodiploid strains containing both mutant and wild-type alleles, indicating that expression of both genes is independent of the wild-type gene product. Examination of catalase zymograms prepared from cells exposed to various levels of acetate revealed that both HPI and HPII catalases are induced by this weak acid, suggesting that there is a common link in the regulation of these two enzymes.

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Selected References

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  • BEERS RF, Jr, SIZER IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem. 1952 Mar;195(1):133–140. [PubMed]
  • Bohannon DE, Connell N, Keener J, Tormo A, Espinosa-Urgel M, Zambrano MM, Kolter R. Stationary-phase-inducible "gearbox" promoters: differential effects of katF mutations and role of sigma 70. J Bacteriol. 1991 Jul;173(14):4482–4492. [PMC free article] [PubMed]
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. [PubMed]
  • Bremer E, Silhavy TJ, Weinstock GM. Transposable lambda placMu bacteriophages for creating lacZ operon fusions and kanamycin resistance insertions in Escherichia coli. J Bacteriol. 1985 Jun;162(3):1092–1099. [PMC free article] [PubMed]
  • Brickman E, Beckwith J. Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and phi80 transducing phages. J Mol Biol. 1975 Aug 5;96(2):307–316. [PubMed]
  • Christman MF, Morgan RW, Jacobson FS, Ames BN. Positive control of a regulon for defenses against oxidative stress and some heat-shock proteins in Salmonella typhimurium. Cell. 1985 Jul;41(3):753–762. [PubMed]
  • Clare DA, Duong MN, Darr D, Archibald F, Fridovich I. Effects of molecular oxygen on detection of superoxide radical with nitroblue tetrazolium and on activity stains for catalase. Anal Biochem. 1984 Aug 1;140(2):532–537. [PubMed]
  • Eisenstark A, Perrot G. Catalase has only a minor role in protection against near-ultraviolet radiation damage in bacteria. Mol Gen Genet. 1987 Apr;207(1):68–72. [PubMed]
  • Hassan HM, Fridovich I. Regulation of the synthesis of catalase and peroxidase in Escherichia coli. J Biol Chem. 1978 Sep 25;253(18):6445–6420. [PubMed]
  • Imlay JA, Linn S. Mutagenesis and stress responses induced in Escherichia coli by hydrogen peroxide. J Bacteriol. 1987 Jul;169(7):2967–2976. [PMC free article] [PubMed]
  • Lange R, Hengge-Aronis R. Growth phase-regulated expression of bolA and morphology of stationary-phase Escherichia coli cells are controlled by the novel sigma factor sigma S. J Bacteriol. 1991 Jul;173(14):4474–4481. [PMC free article] [PubMed]
  • Lange R, Hengge-Aronis R. Identification of a central regulator of stationary-phase gene expression in Escherichia coli. Mol Microbiol. 1991 Jan;5(1):49–59. [PubMed]
  • Linn S, Imlay JA. Toxicity, mutagenesis and stress responses induced in Escherichia coli by hydrogen peroxide. J Cell Sci Suppl. 1987;6:289–301. [PubMed]
  • Loewen PC. Isolation of catalase-deficient Escherichia coli mutants and genetic mapping of katE, a locus that affects catalase activity. J Bacteriol. 1984 Feb;157(2):622–626. [PMC free article] [PubMed]
  • Loewen PC, Switala J, Triggs-Raine BL. Catalases HPI and HPII in Escherichia coli are induced independently. Arch Biochem Biophys. 1985 Nov 15;243(1):144–149. [PubMed]
  • Loewen PC, Triggs BL. Genetic mapping of katF, a locus that with katE affects the synthesis of a second catalase species in Escherichia coli. J Bacteriol. 1984 Nov;160(2):668–675. [PMC free article] [PubMed]
  • Loewen PC, Triggs BL, George CS, Hrabarchuk BE. Genetic mapping of katG, a locus that affects synthesis of the bifunctional catalase-peroxidase hydroperoxidase I in Escherichia coli. J Bacteriol. 1985 May;162(2):661–667. [PMC free article] [PubMed]
  • McCann MP, Kidwell JP, Matin A. The putative sigma factor KatF has a central role in development of starvation-mediated general resistance in Escherichia coli. J Bacteriol. 1991 Jul;173(13):4188–4194. [PMC free article] [PubMed]
  • Mulvey MR, Sorby PA, Triggs-Raine BL, Loewen PC. Cloning and physical characterization of katE and katF required for catalase HPII expression in Escherichia coli. Gene. 1988 Dec 20;73(2):337–345. [PubMed]
  • Mulvey MR, Switala J, Borys A, Loewen PC. Regulation of transcription of katE and katF in Escherichia coli. J Bacteriol. 1990 Dec;172(12):6713–6720. [PMC free article] [PubMed]
  • Sak BD, Eisenstark A, Touati D. Exonuclease III and the catalase hydroperoxidase II in Escherichia coli are both regulated by the katF gene product. Proc Natl Acad Sci U S A. 1989 May;86(9):3271–3275. [PMC free article] [PubMed]
  • Sammartano LJ, Tuveson RW, Davenport R. Control of sensitivity to inactivation by H2O2 and broad-spectrum near-UV radiation by the Escherichia coli katF locus. J Bacteriol. 1986 Oct;168(1):13–21. [PMC free article] [PubMed]
  • Schellhorn HE, Hassan HM. Transcriptional regulation of katE in Escherichia coli K-12. J Bacteriol. 1988 Sep;170(9):4286–4292. [PMC free article] [PubMed]
  • Slonczewski JL, Gonzalez TN, Bartholomew FM, Holt NJ. Mu d-directed lacZ fusions regulated by low pH in Escherichia coli. J Bacteriol. 1987 Jul;169(7):3001–3006. [PMC free article] [PubMed]
  • Touati E, Dassa E, Dassa J, Boquet PL, Touati D. Are appR and katF the same Escherichia coli gene encoding a new sigma transcription initiation factor? Res Microbiol. 1991 Jan;142(1):29–36. [PubMed]
  • Tuveson RW. The interaction of a gene (nur) controlling near-UV sensitivity and the polA1 gene in strains of E. coli K12. Photochem Photobiol. 1981 Jun;33(6):919–923. [PubMed]

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