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J Bacteriol. Jun 1992; 174(12): 4189–4192.
PMCID: PMC206134

Purification and some properties of sulfite:ferric ion oxidoreductase from Thiobacillus ferrooxidans.

Abstract

Sulfite:ferric ion oxidoreductase in the plasma membrane of Thiobacillus ferrooxidans AP19-3 was purified to an electrophoretically homogeneous state. The enzyme had an apparent molecular weight of 650,000 and was composed of two subunits (M(rs), 61,000 and 59,000) as estimated by sodium sulfate-polyacrylamide gel electrophoresis. The Michaelis constants of sulfite:ferric ion oxidoreductase for Fe3+ and sulfite ions were 1.0 and 0.071 mM, respectively. Sulfite:ferric ion oxidoreductase suffered from end product inhibition by 1 mM Fe2+.

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Selected References

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  • Adair FW. Membrane-associated sulfur oxidation by the autotroph Thiobacillus thiooxidans. J Bacteriol. 1966 Oct;92(4):899–904. [PMC free article] [PubMed]
  • LONDON J, RITTENBERG SC. PATH OF SULFUR IN SULFIDE AND THIOSULFATE OXIDATION BY THIOBACILLI. Proc Natl Acad Sci U S A. 1964 Nov;52:1183–1190. [PMC free article] [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Lyric RM, Suzuki I. Enzymes involved in the metabolism of thiosulfate by Thiobacillus thioparus. I. Survey of enzymes and properties of sulfite: cytochrome c oxidoreductase. Can J Biochem. 1970 Mar;48(3):334–343. [PubMed]
  • Moriarty DJ, Nicholas DJ. Electron transfer during sulphide and sulphite oxidation by Thiobacillus concretivorus. Biochim Biophys Acta. 1970 Aug 4;216(1):130–138. [PubMed]
  • Southerland WM, Toghrol F. Sulfite oxidase activity in Thiobacillus novellus. J Bacteriol. 1983 Nov;156(2):941–944. [PMC free article] [PubMed]
  • Sugio T, Domatsu C, Munakata O, Tano T, Imai K. Role of a Ferric Ion-Reducing System in Sulfur Oxidation of Thiobacillus ferrooxidans. Appl Environ Microbiol. 1985 Jun;49(6):1401–1406. [PMC free article] [PubMed]
  • Sugio T, Domatsu C, Tano T, Imai K. Role of Ferrous Ions in Synthetic Cobaltous Sulfide Leaching of Thiobacillus ferrooxidans. Appl Environ Microbiol. 1984 Sep;48(3):461–467. [PMC free article] [PubMed]
  • Sugio T, Katagiri T, Moriyama M, Zhèn YL, Inagaki K, Tano T. Existence of a new type of sulfite oxidase which utilizes ferric ions as an electron acceptor in Thiobacillus ferrooxidans. Appl Environ Microbiol. 1988 Jan;54(1):153–157. [PMC free article] [PubMed]
  • Sugio T, Mizunashi W, Inagaki K, Tano T. Purification and some properties of sulfur:ferric ion oxidoreductase from Thiobacillus ferrooxidans. J Bacteriol. 1987 Nov;169(11):4916–4922. [PMC free article] [PubMed]
  • Sugio T, White KJ, Shute E, Choate D, Blake RC. Existence of a hydrogen sulfide:ferric ion oxidoreductase in iron-oxidizing bacteria. Appl Environ Microbiol. 1992 Jan;58(1):431–433. [PMC free article] [PubMed]
  • Toghrol F, Southerland WM. Purification of Thiobacillus novellus sulfite oxidase. Evidence for the presence of heme and molybdenum. J Biol Chem. 1983 Jun 10;258(11):6762–6766. [PubMed]
  • Vestal JR, Lundgren DG. The sulfite oxidase of Thiobacillus ferrooxidans (Ferrobacillus ferrooxidans). Can J Biochem. 1971 Oct;49(10):1125–1130. [PubMed]
  • Weber K, Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed]

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