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J Bacteriol. Jun 1992; 174(12): 3921–3927.
PMCID: PMC206100

Characterization of cytochromes from Methanosarcina strain Göl and their involvement in electron transport during growth on methanol.

Abstract

Methanosarcina strain Gö1 was tested for the presence of cytochromes. Low-temperature spectroscopy, hemochrome derivative spectroscopy, and redox titration revealed the presence of two b-type (b559 and b564) and two c-type (c547 and c552) cytochromes in membranes from Methanosarcina strain Gö1. The midpoint potentials determined were Em,7 = -135 +/- 5 and -240 +/- 11 mV (b-type cytochromes) and Em,7 = -140 +/- 10 and -230 +/- 10 mV (c-type cytochromes). The protoheme IX and the heme c contents were 0.21 to 0.24 and 0.09 to 0.28 mumol/g of membrane protein, respectively. No cytochromes were detectable in the cytoplasmic fraction. Of various electron donors and acceptors tested, only the reduced form of coenzyme F420 (coenzyme F420H2) and the heterodisulfide of coenzyme M and 7-mercaptoheptanoylthreonine phosphate (CoM-S-S-HTP) were capable of reducing and oxidizing the cytochromes at a high rate, respectively. Addition of CoM-S-S-HTP to reduced cytochromes and subsequent low-temperature spectroscopy revealed the oxidation of cytochrome b564. On the basis of these results, we suggest that one or several cytochromes participate in the coenzyme F420H2-dependent reduction of the heterodisulfide.

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  • Bell GR, LeGall L, Peck HD. Evidence for the periplasmic location of hydrogenase in Desulfovibrio gigas. J Bacteriol. 1974 Nov;120(2):994–997. [PMC free article] [PubMed]
  • Bobik TA, Wolfe RS. Physiological importance of the heterodisulfide of coenzyme M and 7-mercaptoheptanoylthreonine phosphate in the reduction of carbon dioxide to methane in Methanobacterium. Proc Natl Acad Sci U S A. 1988 Jan;85(1):60–63. [PMC free article] [PubMed]
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. [PubMed]
  • Deppenmeier U, Blaut M, Mahlmann A, Gottschalk G. Reduced coenzyme F420: heterodisulfide oxidoreductase, a proton- translocating redox system in methanogenic bacteria. Proc Natl Acad Sci U S A. 1990 Dec 1;87(23):9449–9453. [PMC free article] [PubMed]
  • Dutton PL. Redox potentiometry: determination of midpoint potentials of oxidation-reduction components of biological electron-transfer systems. Methods Enzymol. 1978;54:411–435. [PubMed]
  • Ellermann J, Hedderich R, Böcher R, Thauer RK. The final step in methane formation. Investigations with highly purified methyl-CoM reductase (component C) from Methanobacterium thermoautotrophicum (strain Marburg). Eur J Biochem. 1988 Mar 15;172(3):669–677. [PubMed]
  • ELLMAN GL. A colorimetric method for determining low concentrations of mercaptans. Arch Biochem Biophys. 1958 Apr;74(2):443–450. [PubMed]
  • ESTABROOK RW. The low temperature spectra of hemoproteins. I. Apparatus and its application to a study of cytochrome c. J Biol Chem. 1956 Dec;223(2):781–794. [PubMed]
  • Haase P, Deppenmeier U, Blaut M, Gottschalk G. Purification and characterization of F420H2-dehydrogenase from Methanolobus tindarius. Eur J Biochem. 1992 Feb 1;203(3):527–531. [PubMed]
  • Hagihara B, Iizuka T. Studies on low temperature spectra of respiratory pigments. I. Measurement of absorption spectra between liquid helium and room temperatures. J Biochem. 1971 Feb;69(2):355–362. [PubMed]
  • Hatchikian EC, Le Gall J. Evidence for the presence of a b-type cytochrome in the sulfate-reducing bacterium Desulfovibrio gigas, and its role in the reduction of fumarate by molecular hydrogen. Biochim Biophys Acta. 1972 Jun 23;267(3):479–484. [PubMed]
  • Hauska G. Elucidation of methanogenesis seems well on its way. Trends Biochem Sci. 1988 Jan;13(1):2–4. [PubMed]
  • Hedderich R, Berkessel A, Thauer RK. Purification and properties of heterodisulfide reductase from Methanobacterium thermoautotrophicum (strain Marburg). Eur J Biochem. 1990 Oct 5;193(1):255–261. [PubMed]
  • Heine-Dobbernack E, Schoberth SM, Sahm H. Relationship of Intracellular Coenzyme F(420) Content to Growth and Metabolic Activity of Methanobacterium bryantii and Methanosarcina barkeri. Appl Environ Microbiol. 1988 Feb;54(2):454–459. [PMC free article] [PubMed]
  • Hippe H, Caspari D, Fiebig K, Gottschalk G. Utilization of trimethylamine and other N-methyl compounds for growth and methane formation by Methanosarcina barkeri. Proc Natl Acad Sci U S A. 1979 Jan;76(1):494–498. [PMC free article] [PubMed]
  • JACOBS NJ, WOLIN MJ. Electron-transport system of Vibrio succinogenes. I. Enzymes and cytochromes of electron-transport system. Biochim Biophys Acta. 1963 Jan 1;69:18–28. [PubMed]
  • Kühn W, Fiebig K, Walther R, Gottschalk G. Presence of a cytochrome b559 in Methanosarcina barkeri. FEBS Lett. 1979 Sep 15;105(2):271–274. [PubMed]
  • Kühn W, Gottschalk G. Characterization of the cytochromes occurring in Methanosarcina species. Eur J Biochem. 1983 Sep 1;135(1):89–94. [PubMed]
  • Mayer F, Rohde M, Salzmann M, Jussofie A, Gottschalk G. The methanoreductosome: a high-molecular-weight enzyme complex in the methanogenic bacterium strain Gö1 that contains components of the methylreductase system. J Bacteriol. 1988 Apr;170(4):1438–1444. [PMC free article] [PubMed]
  • Noll KM, Donnelly MI, Wolfe RS. Synthesis of 7-mercaptoheptanoylthreonine phosphate and its activity in the methylcoenzyme M methylreductase system. J Biol Chem. 1987 Jan 15;262(2):513–515. [PubMed]
  • Odom JM, Peck HD., Jr Hydrogenase, electron-transfer proteins, and energy coupling in the sulfate-reducing bacteria Desulfovibrio. Annu Rev Microbiol. 1984;38:551–592. [PubMed]
  • PORRA RJ, JONES OT. Studies on ferrochelatase. 2. An in vestigation of the role offerrochelatase in the biosynthesis of various haem prosthetic groups. Biochem J. 1963 Apr;87:186–192. [PMC free article] [PubMed]
  • Probst I, Schlegel HG. Respiratory components and oxidase activities in Alcaligenes eutrophus. Biochim Biophys Acta. 1976 Aug 13;440(2):412–428. [PubMed]
  • Rouvière PE, Wolfe RS. Novel biochemistry of methanogenesis. J Biol Chem. 1988 Jun 15;263(17):7913–7916. [PubMed]
  • Terlesky KC, Ferry JG. Ferredoxin requirement for electron transport from the carbon monoxide dehydrogenase complex to a membrane-bound hydrogenase in acetate-grown Methanosarcina thermophila. J Biol Chem. 1988 Mar 25;263(9):4075–4079. [PubMed]
  • Wilson GS. Determination of oxidation-reduction potentials. Methods Enzymol. 1978;54:396–410. [PubMed]
  • Wood PM. Bacterial proteins with CO-binding b- or c-type haem. Functions and absorption spectroscopy. Biochim Biophys Acta. 1984 Dec 17;768(3-4):293–317. [PubMed]
  • Zahler WL, Cleland WW. A specific and sensitive assay for disulfides. J Biol Chem. 1968 Feb 25;243(4):716–719. [PubMed]
  • Zannoni D, Melandri BA, Baccarini-Melandri A. Energy tranduction in photosynthetic bacteria. XI. Further resolution of cytochromes of b type and the nature of the co-sensitive oxidase present in the respiratory chain of Rhodopseudomonas capsulata. Biochim Biophys Acta. 1976 Dec 6;449(3):386–400. [PubMed]

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