• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Apr 1992; 174(7): 2199–2207.
PMCID: PMC205839

Physical and genetic mapping of the genomes of five Mycoplasma hominis strains by pulsed-field gel electrophoresis.

Abstract

We present the complete maps of five Mycoplasma hominis genomes, including a detailed restriction map and the locations of a number of genetic loci. The restriction fragments were resolved by field inversion gel electrophoresis or by the contour-clamped homogeneous-electric-field system of pulsed-field gel electrophoresis. All the ApaI, SmaI, BamHI, XhoI, and SalI restriction sites (total of 21 to 33 sites in each strain) were placed on the physical map, yielding an average resolution of 26 kb. The maps were constructed using three different approaches: (i) size determination of DNA fragments partially or completely cleaved with one or two restriction enzymes, (ii) hybridization analysis with purified restriction fragments and specific probes, and (iii) use of linking clones. A genetic map was constructed by hybridization with gene-specific probes for rpoA, rpoC, rrn, tuf, gyrB, hup, ftsY, the unc operon, the genes for two M. hominis-specific antigenic membrane proteins, and one gene encoding a protein with some homology to Escherichia coli alanyl-tRNA synthetase. The positions of mapped loci were partially conserved in the five strains except in one strain in which a 300-kb fragment was inverted. The numbers and order of mapped restriction sites were only partly conserved, and this conservation was restricted to certain regions. The gene order was compared with the gene order established for other bacteria and was found to be identical to that of the phylogenetically related Clostridium perfringens. The genome size of the M. hominis strains varied from 704 to 825 kb.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.7M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Andersen H, Birkelund S, Christiansen G, Freundt EA. Electrophoretic analysis of proteins from Mycoplasma hominis strains detected by SDS-PAGE, two-dimensional gel electrophoresis and immunoblotting. J Gen Microbiol. 1987 Jan;133(1):181–191. [PubMed]
  • Bak AL, Black FT, Christiansen C, Freundt EA. Genome size of mycoplasmal DNA. Nature. 1969 Dec 20;224(5225):1209–1210. [PubMed]
  • Bancroft I, Wolk CP, Oren EV. Physical and genetic maps of the genome of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. J Bacteriol. 1989 Nov;171(11):5940–5948. [PMC free article] [PubMed]
  • Barile MF, Grabowski MW, Stephens EB, O'Brien SJ, Simonson JM, Izumikawa K, Chandler DK, Taylor-Robinson D, Tully JG. Mycoplasma hominis- tissue cell interactions: a review with new observations on phenotypic and genotypic properties. Sex Transm Dis. 1983 Oct-Dec;10(4 Suppl):345–354. [PubMed]
  • Bautsch W. Rapid physical mapping of the Mycoplasma mobile genome by two-dimensional field inversion gel electrophoresis techniques. Nucleic Acids Res. 1988 Dec 23;16(24):11461–11467. [PMC free article] [PubMed]
  • Bode HR, Morowitz HJ. Size and structure of the Mycoplasma hominis H39 chromosome. J Mol Biol. 1967 Jan 28;23(2):191–199. [PubMed]
  • Brewer BJ. When polymerases collide: replication and the transcriptional organization of the E. coli chromosome. Cell. 1988 Jun 3;53(5):679–686. [PubMed]
  • Butler PD, Moxon ER. A physical map of the genome of Haemophilus influenzae type b. J Gen Microbiol. 1990 Dec;136(12):2333–2342. [PubMed]
  • Canard B, Cole ST. Genome organization of the anaerobic pathogen Clostridium perfringens. Proc Natl Acad Sci U S A. 1989 Sep;86(17):6676–6680. [PMC free article] [PubMed]
  • Chang N, Taylor DE. Use of pulsed-field agarose gel electrophoresis to size genomes of Campylobacter species and to construct a SalI map of Campylobacter jejuni UA580. J Bacteriol. 1990 Sep;172(9):5211–5217. [PMC free article] [PubMed]
  • Christiansen C, Frydenberg J, Christiansen G, Andersen H, Hedegaard L. Analysis of the mycoplasma genome by recombinant DNA technology. Isr J Med Sci. 1984 Sep;20(9):781–784. [PubMed]
  • Christiansen G, Andersen H, Birkelund S, Freundt EA. Genomic and gene variation in Mycoplasma hominis strains. Isr J Med Sci. 1987 Jun;23(6):595–602. [PubMed]
  • Cocks BG, Pyle LE, Finch LR. A physical map of the genome of Ureaplasma urealyticum 960T with ribosomal RNA loci. Nucleic Acids Res. 1989 Aug 25;17(16):6713–6719. [PMC free article] [PubMed]
  • Colman SD, Hu PC, Litaker W, Bott KF. A physical map of the Mycoplasma genitalium genome. Mol Microbiol. 1990 Apr;4(4):683–687. [PubMed]
  • Devereux J, Haeberli P, Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. [PMC free article] [PubMed]
  • Ely B, Gerardot CJ. Use of pulsed-field-gradient gel electrophoresis to construct a physical map of the Caulobacter crescentus genome. Gene. 1988 Sep 7;68(2):323–333. [PubMed]
  • Hattori M, Sakaki Y. Dideoxy sequencing method using denatured plasmid templates. Anal Biochem. 1986 Feb 1;152(2):232–238. [PubMed]
  • Kauc L, Mitchell M, Goodgal SH. Size and physical map of the chromosome of Haemophilus influenzae. J Bacteriol. 1989 May;171(5):2474–2479. [PMC free article] [PubMed]
  • Krause DC, Mawn CB. Physical analysis and mapping of the Mycoplasma pneumoniae chromosome. J Bacteriol. 1990 Sep;172(9):4790–4797. [PMC free article] [PubMed]
  • Ladefoged SA, Christiansen G. Analysis of the nucleotide sequence of the Mycoplasma hominis tuf gene and its flanking region. FEMS Microbiol Lett. 1991 Apr 15;63(2-3):133–139. [PubMed]
  • Lee JJ, Smith HO, Redfield RJ. Organization of the Haemophilus influenzae Rd genome. J Bacteriol. 1989 Jun;171(6):3016–3024. [PMC free article] [PubMed]
  • Lin JS, Kass EH. Serological reactions of Mycoplasma hominis: differences among mycoplasmacidal, metabolic inhibition, and growth agglutination tests. Infect Immun. 1974 Sep;10(3):535–540. [PMC free article] [PubMed]
  • McClelland M, Jones R, Patel Y, Nelson M. Restriction endonucleases for pulsed field mapping of bacterial genomes. Nucleic Acids Res. 1987 Aug 11;15(15):5985–6005. [PMC free article] [PubMed]
  • Muto A. The genome structure of Mycoplasma capricolum. Isr J Med Sci. 1987 May;23(5):334–341. [PubMed]
  • Neimark HC. Division of mycoplasmas into subgroups. J Gen Microbiol. 1970 Oct;63(2):249–263. [PubMed]
  • Noll KM. Chromosome map of the thermophilic archaebacterium Thermococcus celer. J Bacteriol. 1989 Dec;171(12):6720–6725. [PMC free article] [PubMed]
  • Pyle LE, Finch LR. Preparation and FIGE separation of infrequent restriction fragments from Mycoplasma mycoides DNA. Nucleic Acids Res. 1988 Mar 25;16(5):2263–2268. [PMC free article] [PubMed]
  • Pyle LE, Finch LR. A physical map of the genome of Mycoplasma mycoides subspecies mycoides Y with some functional loci. Nucleic Acids Res. 1988 Jul 11;16(13):6027–6039. [PMC free article] [PubMed]
  • Pyle LE, Taylor T, Finch LR. Genomic maps of some strains within the Mycoplasma mycoides cluster. J Bacteriol. 1990 Dec;172(12):7265–7268. [PMC free article] [PubMed]
  • Rasmussen OF, Christiansen C. Identification of the proton ATPase operon in Mycoplasma strain PG50 by heterologous hybridization. Isr J Med Sci. 1987 May;23(5):393–397. [PubMed]
  • Ratnaningsih E, Dharmsthiti S, Krishnapillai V, Morgan A, Sinclair M, Holloway BW. A combined physical and genetic map of Pseudomonas aeruginosa PAO. J Gen Microbiol. 1990 Dec;136(12):2351–2357. [PubMed]
  • Razin S. Molecular biology and genetics of mycoplasmas (Mollicutes). Microbiol Rev. 1985 Dec;49(4):419–455. [PMC free article] [PubMed]
  • Robertson JA, Pyle LE, Stemke GW, Finch LR. Human ureaplasmas show diverse genome sizes by pulsed-field electrophoresis. Nucleic Acids Res. 1990 Mar 25;18(6):1451–1455. [PMC free article] [PubMed]
  • Römling U, Grothues D, Bautsch W, Tümmler B. A physical genome map of Pseudomonas aeruginosa PAO. EMBO J. 1989 Dec 20;8(13):4081–4089. [PMC free article] [PubMed]
  • Smith CL, Econome JG, Schutt A, Klco S, Cantor CR. A physical map of the Escherichia coli K12 genome. Science. 1987 Jun 12;236(4807):1448–1453. [PubMed]
  • Trevino LB, Haldenwang WG, Baseman JB. Expression of Mycoplasma pneumoniae antigens in Escherichia coli. Infect Immun. 1986 Jul;53(1):129–134. [PMC free article] [PubMed]
  • Ventra L, Weiss AS. Transposon-mediated restriction mapping of the Bacillus subtilis chromosome. Gene. 1989 May 15;78(1):29–36. [PubMed]
  • Weisburg WG, Tully JG, Rose DL, Petzel JP, Oyaizu H, Yang D, Mandelco L, Sechrest J, Lawrence TG, Van Etten J, et al. A phylogenetic analysis of the mycoplasmas: basis for their classification. J Bacteriol. 1989 Dec;171(12):6455–6467. [PMC free article] [PubMed]
  • Yamao F, Muto A, Kawauchi Y, Iwami M, Iwagami S, Azumi Y, Osawa S. UGA is read as tryptophan in Mycoplasma capricolum. Proc Natl Acad Sci U S A. 1985 Apr;82(8):2306–2309. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...