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J Bacteriol. Apr 1992; 174(7): 2124–2130.
PMCID: PMC205829

Involvement of phosphotransacetylase, acetate kinase, and acetyl phosphate synthesis in control of the phosphate regulon in Escherichia coli.

Abstract

Two controls of the phosphate (PHO) regulon require sensor proteins that are protein kinases that phosphorylate the regulator, PhoB, which in turn activates transcription only when phosphorylated. Pi control requires the Pi sensor PhoR; the other control is Pi independent and requires the sensor CreC (formerly called PhoM). Here we describe an additional control of the PHO regulon which is Pi independent and requires neither PhoR nor CreC. This control is regulated by a two-step pathway in carbon metabolism in which acetyl coenzyme A, Pi, and ADP are converted into acetate, coenzyme A, and ATP via the enzymes phosphotransacetylase (Pta) and acetate kinase (AckA). It responds to the synthesis of acetyl phosphate, an intermediate in the Pta-AckA pathway. Since the synthesis of acetyl phosphate via this pathway leads to the incorporation of Pi into ATP, the primary phosphoryl donor in metabolism, we propose that a regulatory coupling(s) may exist between the PHO regulon, which encodes genes for Pi uptake, and genes for enzymes in central metabolism for incorporation of Pi into ATP. Regulatory interactions of this sort may be important in global control. Further, it provides a functional basis for the concept of cross-regulation in the PHO regulon. This is also the first evidence that acetyl phosphate may have a role as an effector of gene regulation.

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Selected References

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  • Amemura M, Makino K, Shinagawa H, Nakata A. Cross talk to the phosphate regulon of Escherichia coli by PhoM protein: PhoM is a histidine protein kinase and catalyzes phosphorylation of PhoB and PhoM-open reading frame 2. J Bacteriol. 1990 Nov;172(11):6300–6307. [PMC free article] [PubMed]
  • Fox DK, Meadow ND, Roseman S. Phosphate transfer between acetate kinase and enzyme I of the bacterial phosphotransferase system. J Biol Chem. 1986 Oct 15;261(29):13498–13503. [PubMed]
  • Guest JR. Anaerobic growth of Escherichia coli K12 with fumarate as terminal electron acceptor. Genetic studies with menaquinone and fluoroacetate-resistant mutants. J Gen Microbiol. 1979 Dec;115(2):259–271. [PubMed]
  • Hong JS, Hunt AG, Masters PS, Lieberman MA. Requirements of acetyl phosphate for the binding protein-dependent transport systems in Escherichia coli. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1213–1217. [PMC free article] [PubMed]
  • Iuchi S, Lin EC. arcA (dye), a global regulatory gene in Escherichia coli mediating repression of enzymes in aerobic pathways. Proc Natl Acad Sci U S A. 1988 Mar;85(6):1888–1892. [PMC free article] [PubMed]
  • Lee TY, Makino K, Shinagawa H, Nakata A. Overproduction of acetate kinase activates the phosphate regulon in the absence of the phoR and phoM functions in Escherichia coli. J Bacteriol. 1990 May;172(5):2245–2249. [PMC free article] [PubMed]
  • LeVine SM, Ardeshir F, Ames GF. Isolation and Characterization of acetate kinase and phosphotransacetylase mutants of Escherichia coli and Salmonella typhimurium. J Bacteriol. 1980 Aug;143(2):1081–1085. [PMC free article] [PubMed]
  • Makino K, Shinagawa H, Amemura M, Kawamoto T, Yamada M, Nakata A. Signal transduction in the phosphate regulon of Escherichia coli involves phosphotransfer between PhoR and PhoB proteins. J Mol Biol. 1989 Dec 5;210(3):551–559. [PubMed]
  • Metcalf WW, Steed PM, Wanner BL. Identification of phosphate starvation-inducible genes in Escherichia coli K-12 by DNA sequence analysis of psi::lacZ(Mu d1) transcriptional fusions. J Bacteriol. 1990 Jun;172(6):3191–3200. [PMC free article] [PubMed]
  • Metcalf WW, Wanner BL. Involvement of the Escherichia coli phn (psiD) gene cluster in assimilation of phosphorus in the form of phosphonates, phosphite, Pi esters, and Pi. J Bacteriol. 1991 Jan;173(2):587–600. [PMC free article] [PubMed]
  • Ninfa AJ. Protein phosphorylation and the regulation of cellular processes by the homologous two-component regulatory systems of bacteria. Genet Eng (N Y) 1991;13:39–72. [PubMed]
  • Roseman S, Meadow ND. Signal transduction by the bacterial phosphotransferase system. Diauxie and the crr gene (J. Monod revisited). J Biol Chem. 1990 Feb 25;265(6):2993–2996. [PubMed]
  • Singer M, Baker TA, Schnitzler G, Deischel SM, Goel M, Dove W, Jaacks KJ, Grossman AD, Erickson JW, Gross CA. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol Rev. 1989 Mar;53(1):1–24. [PMC free article] [PubMed]
  • Stock JB, Ninfa AJ, Stock AM. Protein phosphorylation and regulation of adaptive responses in bacteria. Microbiol Rev. 1989 Dec;53(4):450–490. [PMC free article] [PubMed]
  • Wanner BL. Novel regulatory mutants of the phosphate regulon in Escherichia coli K-12. J Mol Biol. 1986 Sep 5;191(1):39–58. [PubMed]
  • Wanner BL. Control of phoR-dependent bacterial alkaline phosphatase clonal variation by the phoM region. J Bacteriol. 1987 Feb;169(2):900–903. [PMC free article] [PubMed]
  • Wanner BL. Is cross regulation by phosphorylation of two-component response regulator proteins important in bacteria? J Bacteriol. 1992 Apr;174(7):2053–2058. [PMC free article] [PubMed]
  • Wanner BL, Boline JA. Mapping and molecular cloning of the phn (psiD) locus for phosphonate utilization in Escherichia coli. J Bacteriol. 1990 Mar;172(3):1186–1196. [PMC free article] [PubMed]
  • Wanner BL, Wilmes MR, Hunter E. Molecular cloning of the wild-type phoM operon in Escherichia coli K-12. J Bacteriol. 1988 Jan;170(1):279–288. [PMC free article] [PubMed]
  • Wanner BL, Wilmes MR, Young DC. Control of bacterial alkaline phosphatase synthesis and variation in an Escherichia coli K-12 phoR mutant by adenyl cyclase, the cyclic AMP receptor protein, and the phoM operon. J Bacteriol. 1988 Mar;170(3):1092–1102. [PMC free article] [PubMed]

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