• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Mar 1994; 176(6): 1793–1795.
PMCID: PMC205273

"Protease I" of Escherichia coli functions as a thioesterase in vivo.


Escherichia coli protease I is assayed as an esterase active with certain synthetic model chymotrypsin substrates. However, the gene encoding protease I has the same DNA sequence and genomic location as tesA, a gene that encodes E. coli thioesterase I. We report that both hydrolase activities utilize the same active site and demonstrate that the protein functions as a thioesterase in vivo.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (626K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Barnes EM, Jr, Wakil SJ, Swindell AC. Purification and properties of a palmityl thioesterase II from Escherichia coli. J Biol Chem. 1970 Jun;245(12):3122–3128. [PubMed]
  • Barnes EM, Jr, Wakil SJ. Studies on the mechanism of fatty acid synthesis. XIX. Preparation and general properties of palmityl thioesterase. J Biol Chem. 1968 Jun 10;243(11):2955–2962. [PubMed]
  • BLIGH EG, DYER WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. [PubMed]
  • Cho H, Cronan JE., Jr Escherichia coli thioesterase I, molecular cloning and sequencing of the structural gene and identification as a periplasmic enzyme. J Biol Chem. 1993 May 5;268(13):9238–9245. [PubMed]
  • Ichihara S, Matsubara Y, Kato C, Akasaka K, Mizushima S. Molecular cloning, sequencing, and mapping of the gene encoding protease I and characterization of proteinase and proteinase-defective Escherichia coli mutants. J Bacteriol. 1993 Feb;175(4):1032–1037. [PMC free article] [PubMed]
  • Kass LR, Brock DJ, Bloch K. Beta-hydroxydecanoyl thioester dehydrase. I. Purification and properties. J Biol Chem. 1967 Oct 10;242(19):4418–4431. [PubMed]
  • Kowit JD, Choy WN, Champe SP, Goldberg AL. Role and location of "protease I" from Escherichia coli. J Bacteriol. 1976 Dec;128(3):776–784. [PMC free article] [PubMed]
  • Kragelund BB, Andersen KV, Madsen JC, Knudsen J, Poulsen FM. Three-dimensional structure of the complex between acyl-coenzyme A binding protein and palmitoyl-coenzyme A. J Mol Biol. 1993 Apr 20;230(4):1260–1277. [PubMed]
  • Miller CG, Heiman C, Yen C. Mutants of Salmonella typhimurium deficient in an endoprotease. J Bacteriol. 1976 Jul;127(1):490–497. [PMC free article] [PubMed]
  • Naggert J, Narasimhan ML, DeVeaux L, Cho H, Randhawa ZI, Cronan JE, Jr, Green BN, Smith S. Cloning, sequencing, and characterization of Escherichia coli thioesterase II. J Biol Chem. 1991 Jun 15;266(17):11044–11050. [PubMed]
  • Nunn WD. A molecular view of fatty acid catabolism in Escherichia coli. Microbiol Rev. 1986 Jun;50(2):179–192. [PMC free article] [PubMed]
  • Pacaud M, Sibilli S, Bras G. Protease I from Escherichia coli. Some physicochemical properties and substrate specificity. Eur J Biochem. 1976 Oct 1;69(1):141–151. [PubMed]
  • Pacaud M, Uriel J. Isolation and some propeties of a proteolytic enzyme from Escherichia coli (protease I). Eur J Biochem. 1971 Dec 10;23(3):435–442. [PubMed]
  • Rosendal J, Ertbjerg P, Knudsen J. Characterization of ligand binding to acyl-CoA-binding protein. Biochem J. 1993 Mar 1;290(Pt 2):321–326. [PMC free article] [PubMed]
  • Spencer AK, Greenspan AD, Cronan JE., Jr Thioesterases I and II of Escherichia coli. Hydrolysis of native acyl-acyl carrier protein thioesters. J Biol Chem. 1978 Sep 10;253(17):5922–5926. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...