Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Feb 1994; 176(4): 1087–1092.
PMCID: PMC205160

Physical and genetic map of the Serpulina hyodysenteriae B78T chromosome.

Abstract

A combined physical and genetic map of the Serpulina hyodysenteriae B78T genome was constructed by using pulsed-field gel electrophoresis and DNA blot hybridizations. The S. hyodysenteriae genome is a single circular chromosome about 3.2 Mb in size. The physical map of the chromosome was constructed with the restriction enzymes BssHII, EclXI, NotI, SalI, and SmaI. The physical map was used to constructed a linkage map for genes encoding rRNA, flagellum subunit proteins, DNA gyrase, NADH oxidase, and three distinct hemolysins. Several flaB2-related loci, encoding core flagellum subunit proteins, were detected and are dispersed around the chromosome. The rRNA gene organization in S. hyodysenteriae is unusual. S. hyodysenteriae has one gene each for 5S (rrf), 16S (rrs), and 23S (rrl) rRNAs. The rrf and rrl genes are closely linked (within 5 kb), while the rrs gene is about 860 kb from the other two rRNA genes. Using a probe for the S. hyodysenteriae gyrA gene, we identified a possible location for the chromosomal replication origin. The size and genetic organization of the S. hyodysenteriae chromosome are different from those of previously characterized spirochetes.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Baril C, Herrmann JL, Richaud C, Margarita D, Girons IS. Scattering of the rRNA genes on the physical map of the circular chromosome of Leptospira interrogans serovar icterohaemorrhagiae. J Bacteriol. 1992 Dec;174(23):7566–7571. [PMC free article] [PubMed]
  • Borges KM, Bergquist PL. Genomic restriction map of the extremely thermophilic bacterium Thermus thermophilus HB8. J Bacteriol. 1993 Jan;175(1):103–110. [PMC free article] [PubMed]
  • Brahamsha B, Greenberg EP. Cloning and sequence analysis of flaA, a gene encoding a Spirochaeta aurantia flagellar filament surface antigen. J Bacteriol. 1989 Mar;171(3):1692–1697. [PMC free article] [PubMed]
  • Casjens S, Huang WM. Linear chromosomal physical and genetic map of Borrelia burgdorferi, the Lyme disease agent. Mol Microbiol. 1993 May;8(5):967–980. [PubMed]
  • Champion CI, Miller JN, Lovett MA, Blanco DR. Cloning, sequencing, and expression of two class B endoflagellar genes of Treponema pallidum subsp. pallidum encoding the 34.5- and 31.0-kilodalton proteins. Infect Immun. 1990 Jun;58(6):1697–1704. [PMC free article] [PubMed]
  • Chen X, Finch LR. Novel arrangement of rRNA genes in Mycoplasma gallisepticum: separation of the 16S gene of one set from the 23S and 5S genes. J Bacteriol. 1989 May;171(5):2876–2878. [PMC free article] [PubMed]
  • Davidson BE, MacDougall J, Saint Girons I. Physical map of the linear chromosome of the bacterium Borrelia burgdorferi 212, a causative agent of Lyme disease, and localization of rRNA genes. J Bacteriol. 1992 Jun;174(11):3766–3774. [PMC free article] [PubMed]
  • Fukunaga M, Masuzawa T, Okuzako N, Mifuchi I, Yanagihara Y. Linkage of ribosomal RNA genes in Leptospira. Microbiol Immunol. 1990;34(7):565–573. [PubMed]
  • Fukunaga M, Okuzako N, Mifuchi I, Arimitsu Y, Seki M. Organization of the ribosomal RNA genes in Treponema phagedenis and Treponema pallidum. Microbiol Immunol. 1992;36(2):161–167. [PubMed]
  • Fukunaga M, Sohnaka M. Tandem repeat of the 23S and 5S ribosomal RNA genes in Borrelia burgdorferi, the etiological agent of Lyme disease. Biochem Biophys Res Commun. 1992 Mar 31;183(3):952–957. [PubMed]
  • Fukunaga M, Yanagihara Y, Sohnaka M. The 23S/5S ribosomal RNA genes (rrl/rrf) are separate from the 16S ribosomal RNA gene (rrs) in Borrelia burgdorferi, the aetiological agent of Lyme disease. J Gen Microbiol. 1992 May;138(5):871–877. [PubMed]
  • Hartmann RK, Erdmann VA. Thermus thermophilus 16S rRNA is transcribed from an isolated transcription unit. J Bacteriol. 1989 Jun;171(6):2933–2941. [PMC free article] [PubMed]
  • Isaacs RD, Hanke JH, Guzman-Verduzco LM, Newport G, Agabian N, Norgard MV, Lukehart SA, Radolf JD. Molecular cloning and DNA sequence analysis of the 37-kilodalton endoflagellar sheath protein gene of Treponema pallidum. Infect Immun. 1989 Nov;57(11):3403–3411. [PMC free article] [PubMed]
  • Kent KA, Sellwood R, Lemcke RM, Burrows MR, Lysons RJ. Analysis of the axial filaments of Treponema hyodysenteriae by SDS-PAGE and immunoblotting. J Gen Microbiol. 1989 Jun;135(6):1625–1632. [PubMed]
  • Koopman MB, Baats E, de Leeuw OS, van der Zeijst BA, Kusters JG. Molecular analysis of a flagellar core protein gene of Serpulina (Treponema) hyodysenteriae. J Gen Microbiol. 1993 Aug;139(8):1701–1706. [PubMed]
  • Koopman MB, Baats E, van Vorstenbosch CJ, van der Zeijst BA, Kusters JG. The periplasmic flagella of Serpulina (Treponema) hyodysenteriae are composed of two sheath proteins and three core proteins. J Gen Microbiol. 1992 Dec;138(12):2697–2706. [PubMed]
  • Koopman MB, de Leeuw OS, van der Zeijst BM, Kusters JG. Cloning and DNA sequence analysis of a Serpulina (Treponema) hyodysenteriae gene encoding a periplasmic flagellar sheath protein. Infect Immun. 1992 Jul;60(7):2920–2925. [PMC free article] [PubMed]
  • Krawiec S, Riley M. Organization of the bacterial chromosome. Microbiol Rev. 1990 Dec;54(4):502–539. [PMC free article] [PubMed]
  • Liesack W, Stackebrandt E. Evidence for unlinked rrn operons in the Planctomycete Pirellula marina. J Bacteriol. 1989 Sep;171(9):5025–5030. [PMC free article] [PubMed]
  • Limberger RJ, Slivienski LL, Yelton DB, Charon NW. Molecular genetic analysis of a class B periplasmic-flagellum gene of Treponema phagedenis. J Bacteriol. 1992 Oct;174(20):6404–6410. [PMC free article] [PubMed]
  • Miao RM, Fieldsteel AH, Harris DL. Genetics of Treponema: characterization of Treponema hyodysenteriae and its relationship to Treponema pallidum. Infect Immun. 1978 Dec;22(3):736–739. [PMC free article] [PubMed]
  • Miller DP, Toivio-Kinnucan M, Wu G, Wilt GR. Ultrastructural and electrophoretic analysis of Treponema hyodysenteriae axial filaments. Am J Vet Res. 1988 Jun;49(6):786–789. [PubMed]
  • Muir S, Koopman MB, Libby SJ, Joens LA, Heffron F, Kusters JG. Cloning and expression of a Serpula (Treponema) hyodysenteriae hemolysin gene. Infect Immun. 1992 Feb;60(2):529–535. [PMC free article] [PubMed]
  • Old IG, MacDougall J, Saint Girons I, Davidson BE. Mapping of genes on the linear chromosome of the bacterium Borrelia burgdorferi: possible locations for its origin of replication. FEMS Microbiol Lett. 1992 Dec 1;78(2-3):245–250. [PubMed]
  • Paster BJ, Dewhirst FE, Weisburg WG, Tordoff LA, Fraser GJ, Hespell RB, Stanton TB, Zablen L, Mandelco L, Woese CR. Phylogenetic analysis of the spirochetes. J Bacteriol. 1991 Oct;173(19):6101–6109. [PMC free article] [PubMed]
  • Plasterk RH, Simon MI, Barbour AG. Transposition of structural genes to an expression sequence on a linear plasmid causes antigenic variation in the bacterium Borrelia hermsii. Nature. 1985 Nov 21;318(6043):257–263. [PubMed]
  • Saint Girons I, Norris SJ, Göbel U, Meyer J, Walker EM, Zuerner R. Genome structure of spirochetes. Res Microbiol. 1992 Jul-Aug;143(6):615–621. [PubMed]
  • Schwartz JJ, Gazumyan A, Schwartz I. rRNA gene organization in the Lyme disease spirochete, Borrelia burgdorferi. J Bacteriol. 1992 Jun;174(11):3757–3765. [PMC free article] [PubMed]
  • Stanton TB. Proposal to change the genus designation Serpula to Serpulina gen. nov. containing the species Serpulina hyodysenteriae comb. nov. and Serpulina innocens comb. nov. Int J Syst Bacteriol. 1992 Jan;42(1):189–190. [PubMed]
  • Stanton TB, Jensen NS. Purification and characterization of NADH oxidase from Serpulina (Treponema) hyodysenteriae. J Bacteriol. 1993 May;175(10):2980–2987. [PMC free article] [PubMed]
  • Stanton TB, Jensen NS, Casey TA, Tordoff LA, Dewhirst FE, Paster BJ. Reclassification of Treponema hyodysenteriae and Treponema innocens in a new genus, Serpula gen. nov., as Serpula hyodysenteriae comb. nov. and Serpula innocens comb. nov. Int J Syst Bacteriol. 1991 Jan;41(1):50–58. [PubMed]
  • ter Huurne AA, van Houten M, Muir S, Kusters JG, van der Zeijst BA, Gaastra W. Inactivation of a Serpula (Treponema) hyodysenteriae hemolysin gene by homologous recombination: importance of this hemolysin in pathogenesis in mice. FEMS Microbiol Lett. 1992 Apr 1;71(1):109–113. [PubMed]
  • Thiermann AB, Handsaker AL, Moseley SL, Kingscote B. New method for classification of leptospiral isolates belonging to serogroup pomona by restriction endonuclease analysis: serovar kennewicki. J Clin Microbiol. 1985 Apr;21(4):585–587. [PMC free article] [PubMed]
  • Walker EM, Arnett JK, Heath JD, Norris SJ. Treponema pallidum subsp. pallidum has a single, circular chromosome with a size of approximately 900 kilobase pairs. Infect Immun. 1991 Jul;59(7):2476–2479. [PMC free article] [PubMed]
  • Woese CR. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. [PMC free article] [PubMed]
  • Zuerner RL. Physical map of chromosomal and plasmid DNA comprising the genome of Leptospira interrogans. Nucleic Acids Res. 1991 Sep 25;19(18):4857–4860. [PMC free article] [PubMed]
  • Zuerner RL, Bolin CA. Repetitive sequence element cloned from Leptospira interrogans serovar hardjo type hardjo-bovis provides a sensitive diagnostic probe for bovine leptospirosis. J Clin Microbiol. 1988 Dec;26(12):2495–2500. [PMC free article] [PubMed]
  • Zuerner RL, Herrmann JL, Saint Girons I. Comparison of genetic maps for two Leptospira interrogans serovars provides evidence for two chromosomes and intraspecies heterogeneity. J Bacteriol. 1993 Sep;175(17):5445–5451. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...