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J Bacteriol. Jan 1994; 176(2): 388–394.
PMCID: PMC205061

Rickettsial relative associated with male killing in the ladybird beetle (Adalia bipunctata).

Abstract

A cytoplasmically inherited microorganism associated with male killing in the two-spot ladybird beetle, Adalia bipunctata, is shown to be closely related to bacteria in the genus Rickettsia. Sequencing of a PCR-amplified product of the 16S genes coding for rRNA (16S rDNA) shows the organism associated with male killing in ladybirds to share a common ancestry with the Rickettsias relative to other genera (e.g., Anaplasma, Ehrlichia, and Cowdria). The rickettsial 16S rDNA product is found in four strains of ladybird beetle showing male embryo lethality and is absent from two uninfected strains and an antibiotic-cured strain. In addition, a revertant strain that had naturally lost the male-killing trait failed to amplify the rickettsial 16S rDNA product. Use of PCR primers for a 17-kDa protein antigen which is found only in rickettsias also resulted in an amplified product from infected strains. Uninfected, cured, and revertant strains and insect species infected with related bacteria (cytoplasmic-incompatibility bacteria from Nasonia wasps) failed to amplify the product. Discovery of a close relative of rickettsias associated with sex ratio distortion in insects has implications for the evolution and population dynamics of this bacterial genus.

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  • Adams JR, Schmidtmann ET, Azad AF. Infection of colonized cat fleas, Ctenocephalides felis (Bouché), with a rickettsia-like microorganism. Am J Trop Med Hyg. 1990 Oct;43(4):400–409. [PubMed]
  • Anderson BE, Regnery RL, Carlone GM, Tzianabos T, McDade JE, Fu ZY, Bellini WJ. Sequence analysis of the 17-kilodalton-antigen gene from Rickettsia rickettsii. J Bacteriol. 1987 Jun;169(6):2385–2390. [PMC free article] [PubMed]
  • Anderson BE, Tzianabos T. Comparative sequence analysis of a genus-common rickettsial antigen gene. J Bacteriol. 1989 Sep;171(9):5199–5201. [PMC free article] [PubMed]
  • Andreadis TG, Hall DW. Significance of transovarial infections of Amblyospora sp. (Microspora:Thelohaniidae) in relation to parasite maintenance in the mosquito Culex salinarius. J Invertebr Pathol. 1979 Sep;34(2):152–157. [PubMed]
  • Azad AF, Sacci JB, Jr, Nelson WM, Dasch GA, Schmidtmann ET, Carl M. Genetic characterization and transovarial transmission of a typhus-like rickettsia found in cat fleas. Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):43–46. [PMC free article] [PubMed]
  • Azad AF, Webb L, Carl M, Dasch GA. Detection of rickettsiae in arthropod vectors by DNA amplification using the polymerase chain reaction. Ann N Y Acad Sci. 1990;590:557–563. [PubMed]
  • Breeuwer JA, Stouthamer R, Barns SM, Pelletier DA, Weisburg WG, Werren JH. Phylogeny of cytoplasmic incompatibility micro-organisms in the parasitoid wasp genus Nasonia (Hymenoptera: Pteromalidae) based on 16S ribosomal DNA sequences. Insect Mol Biol. 1992;1(1):25–36. [PubMed]
  • Breeuwer JA, Werren JH. Microorganisms associated with chromosome destruction and reproductive isolation between two insect species. Nature. 1990 Aug 9;346(6284):558–560. [PubMed]
  • Farhang-Azad A, Traub R, Baqar S. Transovarial transmission of murine typhus rickettsiae in Xenopsylla cheopis fleas. Science. 1985 Feb 1;227(4686):543–545. [PubMed]
  • Fine PE. On the dynamics of symbiote-dependent cytoplasmic incompatibility in culicine mosquitoes. J Invertebr Pathol. 1978 Jan;31(1):10–18. [PubMed]
  • Neefs JM, Van de Peer Y, Hendriks L, De Wachter R. Compilation of small ribosomal subunit RNA sequences. Nucleic Acids Res. 1990 Apr 25;18 (Suppl):2237–2317. [PMC free article] [PubMed]
  • Olsen GJ, Overbeek R, Larsen N, Marsh TL, McCaughey MJ, Maciukenas MA, Kuan WM, Macke TJ, Xing Y, Woese CR. The Ribosomal Database Project. Nucleic Acids Res. 1992 May 11;20 (Suppl):2199–2200. [PMC free article] [PubMed]
  • O'Neill SL, Giordano R, Colbert AM, Karr TL, Robertson HM. 16S rRNA phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects. Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2699–2702. [PMC free article] [PubMed]
  • Regnery RL, Spruill CL, Plikaytis BD. Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes. J Bacteriol. 1991 Mar;173(5):1576–1589. [PMC free article] [PubMed]
  • Roberts LW, Rapmund G, Cadigan FC., Jr Sex ratios in rickettsia tsutsugamushi-infected and noninfected colonies of Leptotrombidium (Acari: trombiculidae). J Med Entomol. 1977 Aug 20;14(1):89–92. [PubMed]
  • Rousset F, Bouchon D, Pintureau B, Juchault P, Solignac M. Wolbachia endosymbionts responsible for various alterations of sexuality in arthropods. Proc Biol Sci. 1992 Nov 23;250(1328):91–98. [PubMed]
  • Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987 Jul;4(4):406–425. [PubMed]
  • Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol. 1991 Jan;173(2):697–703. [PMC free article] [PubMed]
  • Weisburg WG, Dobson ME, Samuel JE, Dasch GA, Mallavia LP, Baca O, Mandelco L, Sechrest JE, Weiss E, Woese CR. Phylogenetic diversity of the Rickettsiae. J Bacteriol. 1989 Aug;171(8):4202–4206. [PMC free article] [PubMed]
  • Werren JH, Skinner SW, Huger AM. Male-killing bacteria in a parasitic wasp. Science. 1986 Feb 28;231(4741):990–992. [PubMed]
  • Williams SG, Sacci JB, Jr, Schriefer ME, Andersen EM, Fujioka KK, Sorvillo FJ, Barr AR, Azad AF. Typhus and typhuslike rickettsiae associated with opossums and their fleas in Los Angeles County, California. J Clin Microbiol. 1992 Jul;30(7):1758–1762. [PMC free article] [PubMed]
  • Woese CR. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. [PMC free article] [PubMed]
  • Yen JH, Barr AR. The etiological agent of cytoplasmic incompatibility in Culex pipiens. J Invertebr Pathol. 1973 Sep;22(2):242–250. [PubMed]

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