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J Bacteriol. Jul 1993; 175(14): 4550–4553.
PMCID: PMC204898

Extent of peptidoglycan O acetylation in the tribe Proteeae.


The degree of peptidoglycan O acetylation in 18 strains of the different genera of the tribe Proteeae (Proteus, Providencia, and Morganella) has been determined by high-performance liquid chromatography-based organic acid analysis of mild-base-released acetic acid and quantitation of peptidoglycan concentrations by simultaneous amino sugar-amino acid analysis using high-performance anion-exchange chromatography with pulsed amperometric detection. The N,O-diacetylmuramyl content of all isolated and purified peptidoglycans was greater than 29% and ranged up to 57% relative to total muramic acid concentration. Each of the O-acetylated peptidoglycans was found to be resistant to solubilization by hen egg white lysozyme.

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  • BRUMFITT W. The mechanism of development of resistance to lysozyme by some gram-positive bacteria and its results. Br J Exp Pathol. 1959 Oct;40:441–451. [PMC free article] [PubMed]
  • Clarke AJ, Dupont C. O-acetylated peptidoglycan: its occurrence, pathobiological significance, and biosynthesis. Can J Microbiol. 1992 Feb;38(2):85–91. [PubMed]
  • Dupont C, Clarke AJ. Dependence of lysozyme-catalysed solubilization of Proteus mirabilis peptidoglycan on the extent of O-acetylation. Eur J Biochem. 1991 Feb 14;195(3):763–769. [PubMed]
  • Esser RE, Stimpson SA, Cromartie WJ, Schwab JH. Reactivation of streptococcal cell wall-induced arthritis by homologous and heterologous cell wall polymers. Arthritis Rheum. 1985 Dec;28(12):1402–1411. [PubMed]
  • Fleming TJ, Wallsmith DE, Rosenthal RS. Arthropathic properties of gonococcal peptidoglycan fragments: implications for the pathogenesis of disseminated gonococcal disease. Infect Immun. 1986 May;52(2):600–608. [PMC free article] [PubMed]
  • Fox A, Brown RR, Anderle SK, Chetty C, Cromartie WJ, Gooder H, Schwab JH. Arthropathic properties related to the molecular weight of peptidoglycan-polysaccharide polymers of streptococcal cell walls. Infect Immun. 1982 Mar;35(3):1003–1010. [PMC free article] [PubMed]
  • Ginsburg I, Sela MN. The role of leukocytes and their hydrolases in the persistence, degradation, and transport of bacterial constituents in tissues: relation to chronic inflammatory processes in staphylococcal, streptococcal, and mycobacterial infections and in chronic periodontal disease. CRC Crit Rev Microbiol. 1976 Mar;4(3):249–322. [PubMed]
  • Hamada S, Torii M, Kotani S, Masuda N, Ooshima T, Yokogawa K, Kawata S. Lysis of Streptococcus mutans cells with mutanolysin, a lytic enzyme prepared from a culture liquor of Streptomyces globisporus 1829. Arch Oral Biol. 1978;23(7):543–549. [PubMed]
  • Hash JH. Measurement of bacteriolytic enzymes. J Bacteriol. 1967 Mar;93(3):1201–1202. [PMC free article] [PubMed]
  • Hash JH, Rothlauf MV. The N,O-diacetylmuramidase of Chalaropsis species. I. Purification and crystallization. J Biol Chem. 1967 Dec 10;242(23):5586–5590. [PubMed]
  • Hoyle BD, Beveridge TJ. Metal binding by the peptidoglycan sacculus of Escherichia coli K-12. Can J Microbiol. 1984 Feb;30(2):204–211. [PubMed]
  • Janusz MJ, Esser RE, Schwab JH. In vivo degradation of bacterial cell wall by the muralytic enzyme mutanolysin. Infect Immun. 1986 May;52(2):459–467. [PMC free article] [PubMed]
  • Koga T, Kakimoto K, Hirofuji T, Kotani S, Ohkuni H, Watanabe K, Okada N, Okada H, Sumiyoshi A, Saisho K. Acute joint inflammation in mice after systemic injection of the cell wall, its peptidoglycan, and chemically defined peptidoglycan subunits from various bacteria. Infect Immun. 1985 Oct;50(1):27–34. [PMC free article] [PubMed]
  • Martin HH, Gmeiner J. Modification of peptidoglycan structure by penicillin action in cell walls of Proteus mirabilis. Eur J Biochem. 1979 Apr;95(3):487–495. [PubMed]
  • Rosenthal RS, Blundell JK, Perkins HR. Strain-related differences in lysozyme sensitivity and extent of O-acetylation of gonococcal peptidoglycan. Infect Immun. 1982 Aug;37(2):826–829. [PMC free article] [PubMed]
  • Rosenthal RS, Folkening WJ, Miller DR, Swim SC. Resistance of O-acetylated gonococcal peptidoglycan to human peptidoglycan-degrading enzymes. Infect Immun. 1983 Jun;40(3):903–911. [PMC free article] [PubMed]
  • Swim SC, Gfell MA, Wilde CE, 3rd, Rosenthal RS. Strain distribution in extents of lysozyme resistance and O-acetylation of gonococcal peptidoglycan determined by high-performance liquid chromatography. Infect Immun. 1983 Nov;42(2):446–452. [PMC free article] [PubMed]

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