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Appl Environ Microbiol. Nov 1988; 54(11): 2742–2749.
PMCID: PMC204366

Fermentation of peptides and amino acids by a monensin-sensitive ruminal Peptostreptococcus.

Abstract

A monensin-sensitive ruminal peptostreptococcus was able to grow rapidly (growth rate of 0.5/h) on an enzymatic hydrolysate of casein, but less than 23% of the amino acid nitrogen was ever utilized. When an acid hydrolysate was substituted for the enzymatic digest, more than 31% of the nitrogen was converted to ammonia and cell protein. Coculture experiments and synergisms with peptide-degrading strains of Bacteroides ruminicola and Streptococcus bovis indicated that the peptostreptococcus was unable to transport certain peptides or hydrolyze them extracellularly. Leucine, serine, phenylalanine, threonine, and glutamine were deaminated at rates of 349, 258, 102, 95, and 91 nmol/mg of protein per min, respectively. Deamination rates for some other amino acids were increased when the amino acids were provided as pairs of oxidized and reduced amino acids (Stickland reactions), but these rates were still less than 80 nmol/mg of protein per min. In continuous culture (dilution rate of 0.1/h), bacterial dry matter and ammonia production decreased dramatically at a pH of less than 6.0. When dilution rates were increased from 0.08 to 0.32/h (pH 7.0), ammonia production increased while production of bacterial dry matter and protein decreased. These rather peculiar kinetics resulted in a slightly negative estimate of maintenance energy and could not be explained by a change in fermentation products. Approximately 80% of the cell dry matter was protein. When corrections were made for cell composition, the yield of ATP was higher than the theoretical maximum value. It is possible that mechanisms other than substrate-level phosphorylation contributed to the energetics of growth.

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Selected References

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  • ANNISON EF. Nitrogen metabolism in the sheep; protein digestion in the rumen. Biochem J. 1956 Dec;64(4):705–714. [PMC free article] [PubMed]
  • BAILEY RW. The reaction of pentoses with anthrone. Biochem J. 1958 Apr;68(4):669–672. [PMC free article] [PubMed]
  • Bladen HA, Bryant MP, Doetsch RN. A Study of Bacterial Species from the Rumen Which Produce Ammonia from Protein Hydrolyzate. Appl Microbiol. 1961 Mar;9(2):175–180. [PMC free article] [PubMed]
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. [PubMed]
  • BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. [PMC free article] [PubMed]
  • CHANEY AL, MARBACH EP. Modified reagents for determination of urea and ammonia. Clin Chem. 1962 Apr;8:130–132. [PubMed]
  • Chen G, Strobel HJ, Russell JB, Sniffen CJ. Effect of hydrophobicity of utilization of peptides by ruminal bacteria in vitro. Appl Environ Microbiol. 1987 Sep;53(9):2021–2025. [PMC free article] [PubMed]
  • Chen M, Wolin MJ. Effect of monensin and lasalocid-sodium on the growth of methanogenic and rumen saccharolytic bacteria. Appl Environ Microbiol. 1979 Jul;38(1):72–77. [PMC free article] [PubMed]
  • Dimroth P. Sodium ion transport decarboxylases and other aspects of sodium ion cycling in bacteria. Microbiol Rev. 1987 Sep;51(3):320–340. [PMC free article] [PubMed]
  • Donius DA, Simpson ME, Marsh PB. Effect of monensin fed with forage on digestion and the ruminal ecosystem of steers. J Anim Sci. 1976 Jan;42(1):229–234. [PubMed]
  • Elsden SR, Hilton MG. Volatile acid production from threonine, valine, leucine and isoleucine by clostridia. Arch Microbiol. 1978 May 30;117(2):165–172. [PubMed]
  • Elsden SR, Hilton MG, Waller JM. The end products of the metabolism of aromatic amino acids by Clostridia. Arch Microbiol. 1976 Apr 1;107(3):283–288. [PubMed]
  • ELLFOLK N, SYNGE RL. Detection of pyrrolidone carboxylic acid. Biochem J. 1955 Mar;59(3):523–526. [PMC free article] [PubMed]
  • Hilpert W, Schink B, Dimroth P. Life by a new decarboxylation-dependent energy conservation mechanism with Na as coupling ion. EMBO J. 1984 Aug;3(8):1665–1670. [PMC free article] [PubMed]
  • JACKINS HC, BARKER HA. Fermentative processes of the fusiform bacteria. J Bacteriol. 1951 Feb;61(2):101–114. [PMC free article] [PubMed]
  • Lanigan GW. Peptococcus heliotrinreducans, sp. nov., a cytochrome-producing anaerobe which metabolizes pyrrolizidine alkaloids. J Gen Microbiol. 1976 May;94(1):1–10. [PubMed]
  • LEWIS D, ELSDEN SR. The fermentation of L-threonine, L-serine, L-cysteine and acrylic acid by a gram-negative coccus. Biochem J. 1955 Aug;60(4):683–692. [PMC free article] [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • MESSER M. Enzymatic cyclization of L-glutamine and L-glutaminyl peptides. Nature. 1963 Mar 30;197:1299–1299. [PubMed]
  • METZLER DE, SNELL EE. Deamination of serine. II. D-Serine dehydrase, a vitamin B6 enzyme from Escherichia coli. J Biol Chem. 1952 Sep;198(1):363–373. [PubMed]
  • Neijssel OM, Tempest DW. Bioenergetic aspects of aerobic growth of Klebsiella aerogenes NCTC 418 in carbon-limited and carbon-sufficient chemostat culture. Arch Microbiol. 1976 Mar 19;107(2):215–221. [PubMed]
  • NISMAN B. The Stickland reaction. Bacteriol Rev. 1954 Mar;18(1):16–42. [PMC free article] [PubMed]
  • Payne JW, Nisbet TM. Active transport of peptides in bacteria. Biochem Soc Trans. 1980 Dec;8(6):683–685. [PubMed]
  • Pirt SJ. The maintenance energy of bacteria in growing cultures. Proc R Soc Lond B Biol Sci. 1965 Oct 12;163(991):224–231. [PubMed]
  • ROSEN H. A modified ninhydrin colorimetric analysis for amino acids. Arch Biochem Biophys. 1957 Mar;67(1):10–15. [PubMed]
  • Russell JB. Heat production by ruminal bacteria in continuous culture and its relationship to maintenance energy. J Bacteriol. 1986 Nov;168(2):694–701. [PMC free article] [PubMed]
  • Russell JB. A proposed mechanism of monensin action in inhibiting ruminal bacterial growth: effects on ion flux and protonmotive force. J Anim Sci. 1987 May;64(5):1519–1525. [PubMed]
  • Russell JB, Baldwin RL. Comparison of substrate affinities among several rumen bacteria: a possible determinant of rumen bacterial competition. Appl Environ Microbiol. 1979 Mar;37(3):531–536. [PMC free article] [PubMed]
  • Russell JB, Robinson PH. Compositions and characteristics of strains of Streptococcus bovis. J Dairy Sci. 1984 Jul;67(7):1525–1531. [PubMed]
  • Russell JB, Strobel HJ, Chen GJ. Enrichment and isolation of a ruminal bacterium with a very high specific activity of ammonia production. Appl Environ Microbiol. 1988 Apr;54(4):872–877. [PMC free article] [PubMed]
  • Slyter LL. Influence of acidosis on rumen function. J Anim Sci. 1976 Oct;43(4):910–929. [PubMed]
  • Stouthamer AH. A theoretical study on the amount of ATP required for synthesis of microbial cell material. Antonie Van Leeuwenhoek. 1973;39(3):545–565. [PubMed]
  • Thauer RK, Käufer B, Zähringer M, Jungermann K. The reaction of the iron-sulfur protein hydrogenase with carbon monoxide. Eur J Biochem. 1974 Mar 1;42(2):447–452. [PubMed]
  • Van Nevel CJ, Demeyer DI. Effect of monensin on rumen metabolism in vitro. Appl Environ Microbiol. 1977 Sep;34(3):251–257. [PMC free article] [PubMed]
  • WHITELEY HR. Fermentation of amino acids by Micrococcus aerogenes. J Bacteriol. 1957 Sep;74(3):324–330. [PMC free article] [PubMed]
  • Wohlfarth G, Buckel W. A sodium ion gradient as energy source for Peptostreptococcus asaccharolyticus. Arch Microbiol. 1985 Jul;142(2):128–135. [PubMed]

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