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Am J Pathol. 1979 Jun; 95(3): 663–673.
PMCID: PMC2042322

Induction of diabetes in animals by parenteral administration of ferric nitrilotriacetate. A model of experimental hemochromatosis.

Abstract

Rats and rabbits parenterally treated with a large daily dose of ferric nitrilotriacetate manifested diabetic symptoms such as hypergycemia, glycosuria, ketonemia, and ketonuria after approximately 60 days fo treatment. The blood insulin response to oral glucose loading was poor. Heavy iron deposits were found in liver parenchymal cells and in pancreatic exocrine cells, although some iron was deposited in the macrophages and reticuloendothelial cells of the organs. Faint iron staining was found in some pancreatic islet cells, with a reduction in beta granules and weak zinc staining. Cirrhotic liver changes and skin pigment deposition were not observed. Repeated blood withdrawals from ferric-nitrilotriacetate-treated animals resulted in disappearance of hypergycmia, glycosuria, ketonemia, and ketonuria; disappearance of iron from the liver and pancreas; and restoration of islet beta granules to the control level.

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Selected References

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  • FINCH SC, FINCH CA. Idiopathic hemochromatosis, an iron storage disease. A. Iron metabolism in hemochromatosis. Medicine (Baltimore) 1955 Dec;34(4):381–430. [PubMed]
  • FINCH CA, HEGSTED M, KINNEY TD, THOMAS ED, RATH CE, HASKINS D, FINCH S, FLUHARTY RG. Iron metabolism; the pathophysiology of iron storage. Blood. 1950 Nov;5(11):983–1008. [PubMed]
  • GOLBERG L, SMITH JP, MARTIN LE. The effects of intensive and prolonged administration of iron parenterally in animals. Br J Exp Pathol. 1957 Jun;38(3):297–311. [PMC free article] [PubMed]
  • PLATZER RF, YOUNG LE, YUILE CL. Hemosiderosis resembling hemochromatosis following multiple transfusions. Acta Haematol. 1955 Sep;14(3):185–192. [PubMed]
  • NISSIM JA. Experimental siderosis: a study of the distribution, delayed effects and metabolism of massive amounts of various iron preparations. J Pathol Bacteriol. 1953 Jul;66(1):185–204. [PubMed]
  • Lisboa PE. Experimental hepatic cirrhosis in dogs caused by chronic massive iron overload. Gut. 1971 May;12(5):363–368. [PMC free article] [PubMed]
  • SENO S, AWAI M, KOBAYASHI J, OSE S, KIMOTO T. The reticulo-endothelial system (RES), its function in phagocytosis and iron metabolism. Tohoku J Exp Med. 1962 Mar 25;76:179–188. [PubMed]
  • Bates GW, Schlabach MR. The reaction of ferric salts with transferrin. J Biol Chem. 1973 May 10;248(9):3228–3232. [PubMed]
  • Awai M, Chipman B, Brown EB. In vivo evidence for the functional heterogeneity of transferrin-bound iron. I. Studies in normal rats. J Lab Clin Med. 1975 May;85(5):769–784. [PubMed]
  • RENNIE ID, KEEN H, SOUTHON A. A RAPID ENZYME-STRIP METHOD FOR ESTIMATING BLOOD-SUGAR. Lancet. 1964 Oct 24;2(7365):884–886. [PubMed]
  • COHEN SL, LEGG S, BIRD R. ABEDSIDE METHOD OF BLOOD-GLUCOSE ESTIMATION. Lancet. 1964 Oct 24;2(7365):883–884. [PubMed]
  • LOWRY OH, PASSONNEAU JV, HASSELBERGER FX, SCHULZ DW. EFFECT OF ISCHEMIA ON KNOWN SUBSTRATES AND COFACTORS OF THE GLYCOLYTIC PATHWAY IN BRAIN. J Biol Chem. 1964 Jan;239:18–30. [PubMed]
  • FRASER J, FETTER MC, MAST RL, FREE AH. STUDIES WITH A SIMPLIFIED NITROPRUSSIDE TEST FOR KETONE BODIES IN URINE, SERUM, PLASMA, AND MILK. Clin Chim Acta. 1965 Apr;11:372–378. [PubMed]
  • Greider MH, Howell SL, Lacy PE. Isolation and properties of secretory granules from rat islets of Langerhans. II. Ultrastructure of the beta granule. J Cell Biol. 1969 Apr;41(1):162–166. [PMC free article] [PubMed]
  • Boquist L, Lernmark A. Effects on the endocrine pancreas in Chinese hamsters fed zinc deficient diets. Acta Pathol Microbiol Scand. 1969;76(2):215–228. [PubMed]
  • Hershko C, Cook JD, Finch DA. Storage iron kinetics. 3. Study of desferrioxamine action by selective radioiron labels of RE and parenchymal cells. J Lab Clin Med. 1973 Jun;81(6):876–886. [PubMed]
  • MACDONALD RA, PECHET GS. EXPERIMENTAL HEMOCHROMATOSIS IN RATS. Am J Pathol. 1965 Jan;46:85–109. [PMC free article] [PubMed]

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