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Proc Natl Acad Sci U S A. Oct 23, 2007; 104(43): 16886–16891.
Published online Oct 16, 2007. doi:  10.1073/pnas.0708365104
PMCID: PMC2040445
Biochemistry, Cell Biology

Cyclic changes in metabolic state during the life of a yeast cell


Budding yeast undergo robust oscillations in oxygen consumption during continuous growth in a nutrient-limited environment. Using liquid chromatography-mass spectrometry and comprehensive 2D gas chromatography-mass spectrometry-based metabolite profiling methods, we have determined that the intracellular concentrations of many metabolites change periodically as a function of these metabolic cycles. These results reveal the logic of cellular metabolism during different phases of the life of a yeast cell. They may further indicate that oscillation in the abundance of key metabolites might help control the temporal regulation of cellular processes and the establishment of a cycle. Such oscillations in metabolic state might occur during the course of other biological cycles.

Keywords: gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, metabolic cycle, metabolite profiling

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