Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
Appl Environ Microbiol. Jul 1989; 55(7): 1796–1800.
PMCID: PMC202952

Phenotypic and genomic studies of "Cytophaga psychrophila" isolated from diseased rainbow trout (Oncorhynchus mykiss) in France.

Abstract

Five strains of gliding bacteria were isolated in France from farmed diseased rainbow trouts reared at low water temperature. The resemblance of these bacteria to the known fish pathogen "Cytophaga psychrophila" led to their comparative study with reference strain NCMB 1947 and with an American isolate. Morphological, physiological, and biochemical characteristics of the seven strains proved to be similar. Comparison of their DNA by the S1 nuclease DNA-DNA hybridization method showed that the seven strains formed a tight genomic species with DNA relatedness above 90%. This is the first identification of this fish pathogen in a European country. The main phenotypic characteristics differentiating this bacterium from other nonpathogenic gliding bacteria of fish origin include a poor gliding movement, yellow compact or weakly rhizoid colonies on solid media, and the presence of flexirubin-type pigments. The inability to metabolize any carbohydrates, the strong proteolytic activity, the absence of growth in more than 0.5% NaCl, and the tolerance to a maximum temperature of 25 degrees C are also useful characteristics of this group of bacteria.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • ANACKER RL, ORDAL EJ. Study of a bacteriophage infecting the myxobacterium Chondrococcus columnaris. J Bacteriol. 1955 Dec;70(6):738–741. [PMC free article] [PubMed]
  • Brenner DJ, McWhorter AC, Knutson JK, Steigerwalt AG. Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds. J Clin Microbiol. 1982 Jun;15(6):1133–1140. [PMC free article] [PubMed]
  • GABY WL, HADLEY C. Practical laboratory test for the identification of Pseudomonas aeruginosa. J Bacteriol. 1957 Sep;74(3):356–358. [PMC free article] [PubMed]
  • Lewin RA, Lounsbery DM. Isolation, cultivation and characterization of flexibacteria. J Gen Microbiol. 1969 Oct;58(2):145–170. [PubMed]
  • MARMUR J, DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. [PubMed]
  • Michel C, Bassalert JF. Influence de la température sur les résultats de l'antibiogramme pratiqué par la méthode de diffusion en ichtyopathologie. Ann Rech Vet. 1982;13(3):245–250. [PubMed]
  • Mourey A, Kilbertus G. Simple media containing stabilized tributyrin for demonstrating lipolytic bacteria in foods and soils. J Appl Bacteriol. 1976 Feb;40(1):47–51. [PubMed]
  • Owen RJ, Hill LR, Lapage SP. Determination of DNA base compositions from melting profiles in dilute buffers. Biopolymers. 1969;7(4):503–516. [PubMed]
  • Pacha RE. Characteristics of Cytophaga psychrophila (Borg) isolated during outbreaks of bacterial cold-water disease. Appl Microbiol. 1968 Jan;16(1):97–101. [PMC free article] [PubMed]
  • Pacha RE, Porter S. Characteristics of myxobacteria isolated from the surface of freshwater fish. Appl Microbiol. 1968 Dec;16(12):1901–1906. [PMC free article] [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...