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Appl Environ Microbiol. Apr 1988; 54(4): 917–922.
PMCID: PMC202573

Selection of Ethanol-Tolerant Yeast Hybrids in pH-Regulated Continuous Culture


Hybrids between naturally occurring wine yeast strains and laboratory strains were formed as a method of increasing genetic variability to improve the ethanol tolerance of yeast strains. The hybrids were subjected to competition experiments under continuous culture controlled by pH with increasing ethanol concentrations over a wide range to select the fastest-growing strain at any concentration of ethanol. The continuous culture system was obtained by controlling the dilution rate of a chemostat connected to a pH-meter. The nutrient pump of the chemostat was switched on and off in response to the pH of the culture, which was thereby kept near a critical value (pHc). Under these conditions, when the medium was supplemented with ethanol, the ethanol concentration of the culture increased with each pulse of dilution. A hybrid strain was selected by this procedure that was more tolerant than any of the highly ethanol-tolerant wine yeast strains at any concentration of ethanol and was able to grow at up to 16% (vol/vol) ethanol. This improvement in ethanol tolerance led to an increase in both the ethanol production rate and the total amount of ethanol produced.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Aguilera A, Benítez T. Ethanol-sensitive mutants of Saccharomyces cerevisiae. Arch Microbiol. 1986 Jan;143(4):337–344. [PubMed]
  • Aguilera A, Benítez T. Role of mitochondria in ethanol tolerance of Saccharomyces cerevisiae. Arch Microbiol. 1985 Sep;142(4):389–392. [PubMed]
  • Benítez T, Del Castillo L, Aguilera A, Conde J, Cerdáolmedo E. Selection of wine yeasts for growth and fermentation in the presence of ethanol and sucrose. Appl Environ Microbiol. 1983 May;45(5):1429–1436. [PMC free article] [PubMed]
  • Casey GP, Ingledew WM. Ethanol tolerance in yeasts. Crit Rev Microbiol. 1986;13(3):219–280. [PubMed]
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  • Ismail AA, Ali AM. Selection of high ethanol-yielding Saccharomyces. II. Genetics of ethanol tolerance. Folia Microbiol (Praha) 1971;16(5):350–354. [PubMed]

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