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Appl Environ Microbiol. Mar 1988; 54(3): 712–717.
PMCID: PMC202530

Anaerobic and aerobic metabolism of diverse aromatic compounds by the photosynthetic bacterium Rhodopseudomonas palustris.

Abstract

The purple nonsulfur photosynthetic bacterium Rhodopseudomonas palustris used diverse aromatic compounds for growth under anaerobic and aerobic conditions. Many phenolic, dihydroxylated, and methoxylated aromatic acids, as well as aromatic aldehydes and hydroaromatic acids, supported growth of strain CGA001 in both the presence and absence of oxygen. Some compounds were metabolized under only aerobic or under only anaerobic conditions. Two other strains, CGC023 and CGD052, had similar anaerobic substrate utilization patterns, but CGD052 was able to use a slightly larger number of compounds for growth. These results show that R. palustris is far more versatile in terms of aromatic degradation than had been previously demonstrated. A mutant (CGA033) blocked in aerobic aromatic metabolism remained wild type with respect to anaerobic degradative abilities, indicating that separate metabolic pathways mediate aerobic and anaerobic breakdown of diverse aromatics. Another mutant (CGA047) was unable to grow anaerobically on either benzoate or 4-hydroxybenzoate, and these compounds accumulated in growth media when cells were grown on more complex aromatic compounds. This indicates that R. palustris has two major anaerobic routes for aromatic ring fission, one that passes through benzoate and one that passes through 4-hydroxybenzoate.

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Selected References

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  • Balba MT, Evans WC. The methanogenic fermentation of aromatic substrates. Biochem Soc Trans. 1977;5(1):302–304. [PubMed]
  • Berry DF, Francis AJ, Bollag JM. Microbial metabolism of homocyclic and heterocyclic aromatic compounds under anaerobic conditions. Microbiol Rev. 1987 Mar;51(1):43–59. [PMC free article] [PubMed]
  • Bossert ID, Young LY. Anaerobic oxidation of p-cresol by a denitrifying bacterium. Appl Environ Microbiol. 1986 Nov;52(5):1117–1122. [PMC free article] [PubMed]
  • Braun K, Gibson DT. Anaerobic degradation of 2-aminobenzoate (anthranilic acid) by denitrifying bacteria. Appl Environ Microbiol. 1984 Jul;48(1):102–107. [PMC free article] [PubMed]
  • Dutton PL, Evans WC. Dissimilation of aromatic substrates by Rhodopseudomonas palustris. Biochem J. 1967 Aug;104(2):30P–31P. [PMC free article] [PubMed]
  • Dutton PL, Evans WC. The metabolism of aromatic compounds by Rhodopseudomonas palustris. A new, reductive, method of aromatic ring metabolism. Biochem J. 1969 Jul;113(3):525–536. [PMC free article] [PubMed]
  • Frazer AC, Young LY. A gram-negative anaerobic bacterium that utilizes o-methyl substituents of aromatic acids. Appl Environ Microbiol. 1985 May;49(5):1345–1347. [PMC free article] [PubMed]
  • Grbić-Galić D. Fermentative and oxidative transformation of ferulate by a facultatively anaerobic bacterium isolated from sewage sludge. Appl Environ Microbiol. 1985 Oct;50(4):1052–1057. [PMC free article] [PubMed]
  • Grbić-Galić D, Vogel TM. Transformation of toluene and benzene by mixed methanogenic cultures. Appl Environ Microbiol. 1987 Feb;53(2):254–260. [PMC free article] [PubMed]
  • Guyer M, Hegeman G. Evidence for a reductive pathway for the anaerobic metabolism of benzoate. J Bacteriol. 1969 Sep;99(3):906–907. [PMC free article] [PubMed]
  • Harwood CS, Gibson J. Uptake of benzoate by Rhodopseudomonas palustris grown anaerobically in light. J Bacteriol. 1986 Feb;165(2):504–509. [PMC free article] [PubMed]
  • Hegeman GD. The metabolism of p-hydroxybenzoate by Rhodopseudomonas palustris and its regulation. Arch Mikrobiol. 1967;59(1):143–148. [PubMed]
  • Keith CL, Bridges RL, Fina LR, Iverson KL, Cloran JA. The anaerobic decomposition of benzoic acid during methane fermentation. IV. Dearomatization of the ring and volatile fatty acids formed on ring rupture. Arch Microbiol. 1978 Aug 1;118(2):173–176. [PubMed]
  • Ornston LN, Stanier RY. The conversion of catechol and protocatechuate to beta-ketoadipate by Pseudomonas putida. J Biol Chem. 1966 Aug 25;241(16):3776–3786. [PubMed]
  • Schennen U, Braun K, Knackmuss HJ. Anaerobic degradation of 2-fluorobenzoate by benzoate-degrading, denitrifying bacteria. J Bacteriol. 1985 Jan;161(1):321–325. [PMC free article] [PubMed]
  • Sleat R, Robinson JP. The bacteriology of anaerobic degradation of aromatic compounds. J Appl Bacteriol. 1984 Dec;57(3):381–394. [PubMed]
  • Taylor BF. Aerobic and Anaerobic Catabolism of Vanillic Acid and Some Other Methoxy-Aromatic Compounds by Pseudomonas sp. Strain PN-1. Appl Environ Microbiol. 1983 Dec;46(6):1286–1292. [PMC free article] [PubMed]
  • Tschech A, Fuchs G. Anaerobic degradation of phenol by pure cultures of newly isolated denitrifying pseudomonads. Arch Microbiol. 1987 Sep;148(3):213–217. [PubMed]
  • Whittle PJ, Lunt DO, Evans WC. Anaerobic photometabolism of aromatic compounds by Rhodopseudomonas sp. Biochem Soc Trans. 1976;4(3):490–491. [PubMed]
  • Williams RJ, Evans WC. The metabolism of benzoate by Moraxella species through anaerobic nitrate respiration. Evidence for a reductive pathway. Biochem J. 1975 Apr;148(1):1–10. [PMC free article] [PubMed]
  • Zeyer J, Kuhn EP, Schwarzenbach RP. Rapid microbial mineralization of toluene and 1,3-dimethylbenzene in the absence of molecular oxygen. Appl Environ Microbiol. 1986 Oct;52(4):944–947. [PMC free article] [PubMed]

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