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Appl Environ Microbiol. 1993 March; 59(3): 942–944. | PMCID: PMC202216 |
Comparison of chemical assay, bioassay, enzyme-linked immunosorbent assay, and dot blot hybridization for detection of aerobactin in members of the family Enterobacteriaceae. D Le Roy, A Bouchet, P Saulnier, S Pecquet, and A Andremont Laboratoire d'Ecologie Microbienne, Institut Gustave-Roussy, Chatenay-Malabry, France. Abstract In order to determine the best strategy for detection of aerobactin in members of the family Enterobacteriaceae, we compared the results of three phenotypic assays, including a chemical assay, a cross-feeding bioassay, and an enzyme-linked immunosorbent assay (ELISA), with the results of a dot blot hybridization assay using a specific probe for the aerobactin genes. The sensitivity and specificity of the ELISA were better than those of the chemical and cross-feeding assays, but the results of dot blot hybridization were the most reproducible. However, none of the Serratia and Enterobacter cloacae strains which produced aerobactin hybridized with the probe. We concluded that the best strategy for aerobactin detection is a two-step procedure that combines screening by dot blot hybridization with an ELISA for negative strains. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (535K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References. These references are in PubMed. This may not be the complete list of references from this article. - Aumont P, Enard C, Expert D, Pieddeloup C, Tancrède C, Andremont A. Production of haemolysin, aerobactin and enterobactin by strains of Escherichia coli causing bacteraemia in cancer patients, and their resistance to human serum. Res Microbiol. 1989 Jan;140(1):21–26. [PubMed]
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