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Appl Environ Microbiol. 1994 Dec; 60(12): 4584–4586.
PMCID: PMC202023

Detection of β-Glucosidase Activity in Polyacrylamide Gels with Esculin as Substrate


β-Glucosidase can be located after nondenaturing polyacrylamide gel electrophoresis by incubating the gel with 0.1% esculin and 0.03% ferric chloride. The esculetin released from esculin by β-glucosidase action reacts with ferric ion to produce a black band, corresponding to the β-glucosidase, against the transparent background.

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