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Br J Cancer. 1981 Sep; 44(3): 349–355.
PMCID: PMC2010772

Measurement of self-renewal in culture of clonogenic cells from human ovarian carcinoma.


To test the identity of human tumour clonogenic cells and stem cells, a procedure was developed to allow quantitation of self-renewal capacity of human ovarian carcinoma clonogenic cells. Primary colonies grown from malignant effusions of 10 patients were disaggregated and replated; secondary colonies were observed to be similar to primary colonies in size, morphology and culture requirements. Density-gradient separation of tumour-cell populations demonstrated that not all primary clonogenic cells are capable of self-renewal during clonal expansion. Patient-to-patient variation in self-renewal capacity was shown to be significantly correlated with the concentration of the tumour-cell population in the effusion fluid, and preliminary evidence of a progressive increase in self-renewal was found in one patient. It was concluded that some, but not all, ovarian-tumour clonogenic cells have the stem-cell property of self-renewal, and that quantitation of such a property may identify an important prognostic variable.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Buick RN, Minden MD, McCulloch EA. Self-renewal in culture of proliferative blast progenitor cells in acute myeloblastic leukemia. Blood. 1979 Jul;54(1):95–104. [PubMed]
  • Buick RN, Fry SE, Salmon SE. Effect of host-cell interactions on clonogenic carcinoma cells in human malignant effusions. Br J Cancer. 1980 May;41(5):695–704. [PMC free article] [PubMed]
  • Buick RN, Fry SE. A comparison of human tumour-cell clonogenicity in methylcellulose and agar culture. Br J Cancer. 1980 Dec;42(6):933–936. [PMC free article] [PubMed]
  • Hamburger AW, Salmon SE, Kim MB, Trent JM, Soehnlen BJ, Alberts DS, Schmidt HJ. Direct cloning of human ovarian carcinoma cells in agar. Cancer Res. 1978 Oct;38(10):3438–3444. [PubMed]

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