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Appl Environ Microbiol. Aug 1992; 58(8): 2360–2367.
PMCID: PMC195787

Cloning, expression, and nucleotide sequence of genes involved in production of pediocin PA-1, and bacteriocin from Pediococcus acidilactici PAC1.0.

Abstract

The production of pediocin PA-1, a small heat-stable bacteriocin, is associated with the presence of the 9.4-kbp plasmid pSRQ11 in Pediococcus acidilactici PAC1.0. It was shown by subcloning of pSRQ11 in Escherichia coli cloning vectors that pediocin PA-1 is produced and, most probably, secreted by E. coli cells. Deletion analysis showed that a 5.6-kbp SalI-EcoRI fragment derived from pSRQ11 is required for pediocin PA-1 production. Nucleotide sequence analysis of this 5.6-kbp fragment indicated the presence of four clustered open reading frames (pedA, pedB, pedC, and pedD). The pedA gene encodes a 62-amino-acid precursor of pediocin PA-1, as the predicted amino acid residues 19 to 62 correspond entirely to the amino acid sequence of the purified pediocin PA-1. Introduction of a mutation in pedA resulted in a complete loss of pediocin production. The pedB and pedC genes, encoding proteins of 112 and 174 amino acid residues, respectively, are located directly downstream of the pediocin structural gene. Functions could not be assigned to their gene products; mutation analysis showed that the PedB protein is not involved in pediocin PA-1 production. The mutation analysis further revealed that the fourth gene, pedD, specifying a relatively large protein of 724 amino acids, is required for pediocin PA-1 production in E. coli. The predicted pedD protein shows strong similarities to several ATP-dependent transport proteins, including the E. coli hemolysin secretion protein HlyB and the ComA protein, which is required for competence induction for genetic transformation in Streptococcus pneumoniae.(ABSTRACT TRUNCATED AT 250 WORDS)

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  • Biggin MD, Gibson TJ, Hong GF. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963–3965. [PMC free article] [PubMed]
  • Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed]
  • Buchman GW, Banerjee S, Hansen JN. Structure, expression, and evolution of a gene encoding the precursor of nisin, a small protein antibiotic. J Biol Chem. 1988 Nov 5;263(31):16260–16266. [PubMed]
  • Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. [PMC free article] [PubMed]
  • Chung YJ, Steen MT, Hansen JN. The subtilin gene of Bacillus subtilis ATCC 6633 is encoded in an operon that contains a homolog of the hemolysin B transport protein. J Bacteriol. 1992 Feb;174(4):1417–1422. [PMC free article] [PubMed]
  • Clewell DB, Helinski DR. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970 Oct 27;9(22):4428–4440. [PubMed]
  • Daeschel MA, Klaenhammer TR. Association of a 13.6-Megadalton Plasmid in Pediococcus pentosaceus with Bacteriocin Activity. Appl Environ Microbiol. 1985 Dec;50(6):1538–1541. [PMC free article] [PubMed]
  • De Graaf FK, Oudega B. Production and release of cloacin DF13 and related colicins. Curr Top Microbiol Immunol. 1986;125:183–205. [PubMed]
  • Devereux J, Haeberli P, Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. [PMC free article] [PubMed]
  • Dodd HM, Horn N, Gasson MJ. Analysis of the genetic determinant for production of the peptide antibiotic nisin. J Gen Microbiol. 1990 Mar;136(3):555–566. [PubMed]
  • Doolittle RF, Johnson MS, Husain I, Van Houten B, Thomas DC, Sancar A. Domainal evolution of a prokaryotic DNA repair protein and its relationship to active-transport proteins. Nature. 1986 Oct 2;323(6087):451–453. [PubMed]
  • Felmlee T, Pellett S, Welch RA. Nucleotide sequence of an Escherichia coli chromosomal hemolysin. J Bacteriol. 1985 Jul;163(1):94–105. [PMC free article] [PubMed]
  • Gilmore MS. Molecular cloning of genes encoding gram-positive virulence factors. Curr Top Microbiol Immunol. 1985;118:219–234. [PubMed]
  • Gonzalez CF, Kunka BS. Plasmid transfer in Pediococcus spp.: intergeneric and intrageneric transfer of pIP501. Appl Environ Microbiol. 1983 Jul;46(1):81–89. [PMC free article] [PubMed]
  • Gonzalez CF, Kunka BS. Evidence for Plasmid Linkage of Raffinose Utilization and Associated alpha-Galactosidase and Sucrose Hydrolase Activity in Pediococcus pentosaceus. Appl Environ Microbiol. 1986 Jan;51(1):105–109. [PMC free article] [PubMed]
  • Gonzalez CF, Kunka BS. Plasmid-Associated Bacteriocin Production and Sucrose Fermentation in Pediococcus acidilactici. Appl Environ Microbiol. 1987 Oct;53(10):2534–2538. [PMC free article] [PubMed]
  • Graham DC, McKay LL. Plasmid DNA in Strains of Pediococcus cerevisiae and Pediococcus pentosaceus. Appl Environ Microbiol. 1985 Aug;50(2):532–534. [PMC free article] [PubMed]
  • Harmon KS, McKay LL. Restriction enzyme analysis of lactose and bacteriocin plasmids from Streptococcus lactis subsp. diacetylactis WM4 and cloning of BclI fragments coding for bacteriocin production. Appl Environ Microbiol. 1987 May;53(5):1171–1174. [PMC free article] [PubMed]
  • Hastings JW, Sailer M, Johnson K, Roy KL, Vederas JC, Stiles ME. Characterization of leucocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostoc gelidum. J Bacteriol. 1991 Dec;173(23):7491–7500. [PMC free article] [PubMed]
  • Hattori M, Sakaki Y. Dideoxy sequencing method using denatured plasmid templates. Anal Biochem. 1986 Feb 1;152(2):232–238. [PubMed]
  • Henderson JT, Chopko AL, van Wassenaar PD. Purification and primary structure of pediocin PA-1 produced by Pediococcus acidilactici PAC-1.0. Arch Biochem Biophys. 1992 May 15;295(1):5–12. [PubMed]
  • Higgins CF, Hiles ID, Salmond GP, Gill DR, Downie JA, Evans IJ, Holland IB, Gray L, Buckel SD, Bell AW, et al. A family of related ATP-binding subunits coupled to many distinct biological processes in bacteria. Nature. 1986 Oct 2;323(6087):448–450. [PubMed]
  • Holo H, Nilssen O, Nes IF. Lactococcin A, a new bacteriocin from Lactococcus lactis subsp. cremoris: isolation and characterization of the protein and its gene. J Bacteriol. 1991 Jun;173(12):3879–3887. [PMC free article] [PubMed]
  • Hui FM, Morrison DA. Genetic transformation in Streptococcus pneumoniae: nucleotide sequence analysis shows comA, a gene required for competence induction, to be a member of the bacterial ATP-dependent transport protein family. J Bacteriol. 1991 Jan;173(1):372–381. [PMC free article] [PubMed]
  • Joerger MC, Klaenhammer TR. Cloning, expression, and nucleotide sequence of the Lactobacillus helveticus 481 gene encoding the bacteriocin helveticin J. J Bacteriol. 1990 Nov;172(11):6339–6347. [PMC free article] [PubMed]
  • Kaletta C, Entian KD. Nisin, a peptide antibiotic: cloning and sequencing of the nisA gene and posttranslational processing of its peptide product. J Bacteriol. 1989 Mar;171(3):1597–1601. [PMC free article] [PubMed]
  • Klein C, Kaletta C, Schnell N, Entian KD. Analysis of genes involved in biosynthesis of the lantibiotic subtilin. Appl Environ Microbiol. 1992 Jan;58(1):132–142. [PMC free article] [PubMed]
  • Klein P, Kanehisa M, DeLisi C. The detection and classification of membrane-spanning proteins. Biochim Biophys Acta. 1985 May 28;815(3):468–476. [PubMed]
  • Leblanc DJ, Lee LN. Rapid screening procedure for detection of plasmids in streptococci. J Bacteriol. 1979 Dec;140(3):1112–1115. [PMC free article] [PubMed]
  • Macrina FL, Tobian JA, Jones KR, Evans RP, Clewell DB. A cloning vector able to replicate in Escherichia coli and Streptococcus sanguis. Gene. 1982 Oct;19(3):345–353. [PubMed]
  • Mundt JO, Beattie WG, Wieland FR. Pediococci residing on plants. J Bacteriol. 1969 Jun;98(3):938–942. [PMC free article] [PubMed]
  • Muriana PM, Klaenhammer TR. Cloning, phenotypic expression, and DNA sequence of the gene for lactacin F, an antimicrobial peptide produced by Lactobacillus spp. J Bacteriol. 1991 Mar;173(5):1779–1788. [PMC free article] [PubMed]
  • Nielsen JW, Dickson JS, Crouse JD. Use of a bacteriocin produced by Pediococcus acidilactici to inhibit Listeria monocytogenes associated with fresh meat. Appl Environ Microbiol. 1990 Jul;56(7):2142–2145. [PMC free article] [PubMed]
  • Pearson WR, Lipman DJ. Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444–2448. [PMC free article] [PubMed]
  • PEDERSON CS. The genus Pediococcus. Bacteriol Rev. 1949 Dec;13(4):225–232. [PMC free article] [PubMed]
  • Pucci MJ, Vedamuthu ER, Kunka BS, Vandenbergh PA. Inhibition of Listeria monocytogenes by using bacteriocin PA-1 produced by Pediococcus acidilactici PAC 1.0. Appl Environ Microbiol. 1988 Oct;54(10):2349–2353. [PMC free article] [PubMed]
  • Raccach M. Pediococci and biotechnology. Crit Rev Microbiol. 1987;14(4):291–309. [PubMed]
  • Robeson JP, Barletta RG, Curtiss R., 3rd Expression of a Streptococcus mutans glucosyltransferase gene in Escherichia coli. J Bacteriol. 1983 Jan;153(1):211–221. [PMC free article] [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Smorawinska M, Hsu JC, Hansen JB, Jagusztyn-Krynicka EK, Abiko Y, Curtiss R., 3rd Clustered genes for galactose metabolism from Streptococcus mutans cloned in Escherichia coli. J Bacteriol. 1983 Feb;153(2):1095–1097. [PMC free article] [PubMed]
  • Tobian JA, Cline ML, Macrina FL. Characterization and expression of a cloned tetracycline resistance determinant from the chromosome of Streptococcus mutans. J Bacteriol. 1984 Nov;160(2):556–563. [PMC free article] [PubMed]
  • van Belkum MJ, Hayema BJ, Geis A, Kok J, Venema G. Cloning of two bacteriocin genes from a lactococcal bacteriocin plasmid. Appl Environ Microbiol. 1989 May;55(5):1187–1191. [PMC free article] [PubMed]
  • van Belkum MJ, Kok J, Venema G. Cloning, sequencing, and expression in Escherichia coli of lcnB, a third bacteriocin determinant from the lactococcal bacteriocin plasmid p9B4-6. Appl Environ Microbiol. 1992 Feb;58(2):572–577. [PMC free article] [PubMed]
  • von Heijne G. Transcending the impenetrable: how proteins come to terms with membranes. Biochim Biophys Acta. 1988 Jun 9;947(2):307–333. [PubMed]

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