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Appl Environ Microbiol. Jul 1992; 58(7): 2227–2230.
PMCID: PMC195759

Anaerobic Growth of Thiobacillus ferrooxidans

Abstract

The obligately autotrophic acidophile Thiobacillus ferrooxidans was grown on elemental sulfur in anaerobic batch cultures, using ferric iron as an electron acceptor. During anaerobic growth, ferric iron present in the growth media was quantitatively reduced to ferrous iron. The doubling time in anaerobic cultures was approximately 24 h. Anaerobic growth did not occur in the absence of elemental sulfur or ferric iron. During growth, a linear relationship existed between the concentration of ferrous iron accumulated in the cultures and the cell density. The results suggest that ferric iron may be an important electron acceptor for the oxidation of sulfur compounds in acidic environments.

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Selected References

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  • Brock TD, Gustafson J. Ferric iron reduction by sulfur- and iron-oxidizing bacteria. Appl Environ Microbiol. 1976 Oct;32(4):567–571. [PMC free article] [PubMed]
  • Drobner E, Huber H, Stetter KO. Thiobacillus ferrooxidans, a facultative hydrogen oxidizer. Appl Environ Microbiol. 1990 Sep;56(9):2922–2923. [PMC free article] [PubMed]
  • Ingledew WJ. Thiobacillus ferrooxidans. The bioenergetics of an acidophilic chemolithotroph. Biochim Biophys Acta. 1982 Nov 30;683(2):89–117. [PubMed]
  • Johnson DB, McGinness S. Ferric iron reduction by acidophilic heterotrophic bacteria. Appl Environ Microbiol. 1991 Jan;57(1):207–211. [PMC free article] [PubMed]
  • McGoran CJ, Duncan DW, Walden CC. Growth of Thiobacillus ferrooxidans on various substrates. Can J Microbiol. 1969 Jan;15(1):135–138. [PubMed]
  • Muyzer G, de Bruyn AC, Schmedding DJ, Bos P, Westbroek P, Kuenen GJ. A Combined Immunofluorescence-DNA-Fluorescence Staining Technique for Enumeration of Thiobacillus ferrooxidans in a Population of Acidophilic Bacteria. Appl Environ Microbiol. 1987 Apr;53(4):660–664. [PMC free article] [PubMed]
  • Pronk JT, Liem K, Bos P, Kuenen JG. Energy Transduction by Anaerobic Ferric Iron Respiration in Thiobacillus ferrooxidans. Appl Environ Microbiol. 1991 Jul;57(7):2063–2068. [PMC free article] [PubMed]
  • Pronk JT, Meijer WM, Hazeu W, van Dijken JP, Bos P, Kuenen JG. Growth of Thiobacillus ferrooxidans on Formic Acid. Appl Environ Microbiol. 1991 Jul;57(7):2057–2062. [PMC free article] [PubMed]
  • Suzuki I, Takeuchi TL, Yuthasastrakosol TD, Oh JK. Ferrous Iron and Sulfur Oxidation and Ferric Iron Reduction Activities of Thiobacillus ferrooxidans Are Affected by Growth on Ferrous Iron, Sulfur, or a Sulfide Ore. Appl Environ Microbiol. 1990 Jun;56(6):1620–1626. [PMC free article] [PubMed]

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