• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Mar 1993; 175(5): 1272–1277.
PMCID: PMC193211

Evolution of bacteriophage T7 in a growing plaque.

Abstract

The emergence of mutants during the 10(9)-fold amplification of a bacteriophage was spatially resolved in a growing plaque. When wild-type phage T7 was grown on an Escherichia coli host which expressed an essential early enzyme of the phage infection cycle, the T7 RNA polymerase, mutant phage relying on this enzyme appeared in 10(8) phage replications and outgrew the wild type. Spatial resolution of the selection process was achieved by analyzing stab samples taken along a plaque radius. Different mutants were selected at different rates along different radii of the plaque, based on host range and restriction patterns of the isolates. The mutants deleted up to 11% of their genomes, including the gene for their own RNA polymerase. They gained an advantage over the wild type by replicating more efficiently, as determined by one-step growth cultures.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.5M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bauer GJ, McCaskill JS, Otten H. Traveling waves of in vitro evolving RNA. Proc Natl Acad Sci U S A. 1989 Oct;86(20):7937–7941. [PMC free article] [PubMed]
  • Campbell JL, Richardson CC, Studier FW. Genetic recombination and complementation between bacteriophage T7 and cloned fragments of T7 DNA. Proc Natl Acad Sci U S A. 1978 May;75(5):2276–2280. [PMC free article] [PubMed]
  • Chamberlin M, McGrath J, Waskell L. New RNA polymerase from Escherichia coli infected with bacteriophage T7. Nature. 1970 Oct 17;228(5268):227–231. [PubMed]
  • Demerec M, Fano U. Bacteriophage-Resistant Mutants in Escherichia Coli. Genetics. 1945 Mar;30(2):119–136. [PMC free article] [PubMed]
  • Devereux J, Haeberli P, Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. [PMC free article] [PubMed]
  • Dunn JJ, Studier FW. Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements. J Mol Biol. 1983 Jun 5;166(4):477–535. [PubMed]
  • Eigen M. Selforganization of matter and the evolution of biological macromolecules. Naturwissenschaften. 1971 Oct;58(10):465–523. [PubMed]
  • Eigen M, Gardiner W, Schuster P, Winkler-Oswatitsch R. The origin of genetic information. Sci Am. 1981 Apr;244(4):88–passim. [PubMed]
  • Hausmann R. Bacteriophage T7 genetics. Curr Top Microbiol Immunol. 1976;75:77–110. [PubMed]
  • Helling RB, Goodman HM, Boyer HW. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J Virol. 1974 Nov;14(5):1235–1244. [PMC free article] [PubMed]
  • Hillis DM, Bull JJ, White ME, Badgett MR, Molineux IJ. Experimental phylogenetics: generation of a known phylogeny. Science. 1992 Jan 31;255(5044):589–592. [PubMed]
  • McDonell MW, Simon MN, Studier FW. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977 Feb 15;110(1):119–146. [PubMed]
  • Moffatt BA, Studier FW. Entry of bacteriophage T7 DNA into the cell and escape from host restriction. J Bacteriol. 1988 May;170(5):2095–2105. [PMC free article] [PubMed]
  • Parvin JD, Moscona A, Pan WT, Leider JM, Palese P. Measurement of the mutation rates of animal viruses: influenza A virus and poliovirus type 1. J Virol. 1986 Aug;59(2):377–383. [PMC free article] [PubMed]
  • Pierce JC, Masker W. Genetic deletions between directly repeated sequences in bacteriophage T7. Mol Gen Genet. 1989 Jun;217(2-3):215–222. [PubMed]
  • Rosenberg AH, Simon MN, Studier FW, Roberts RJ. Survey and mapping of restriction endonuclease cleavage sites in bacteriophage T7 DNA. J Mol Biol. 1979 Dec 25;135(4):907–915. [PubMed]
  • Scearce LM, Pierce JC, McInroy B, Masker W. Deletion mutagenesis independent of recombination in bacteriophage T7. J Bacteriol. 1991 Jan;173(2):869–878. [PMC free article] [PubMed]
  • Studier FW. The genetics and physiology of bacteriophage T7. Virology. 1969 Nov;39(3):562–574. [PubMed]
  • Studier FW. Genetic analysis of non-essential bacteriophage T7 genes. J Mol Biol. 1973 Sep 15;79(2):227–236. [PubMed]
  • Studier FW, Dunn JJ. Organization and expression of bacteriophage T7 DNA. Cold Spring Harb Symp Quant Biol. 1983;47(Pt 2):999–1007. [PubMed]
  • Studier FW, Moffatt BA. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol. 1986 May 5;189(1):113–130. [PubMed]
  • Studier FW, Rosenberg AH, Simon MN, Dunn JJ. Genetic and physical mapping in the early region of bacteriophage T7 DNA. J Mol Biol. 1979 Dec 25;135(4):917–937. [PubMed]
  • Yin J. A quantifiable phenotype of viral propagation. Biochem Biophys Res Commun. 1991 Jan 31;174(2):1009–1014. [PubMed]
  • Yin J, McCaskill JS. Replication of viruses in a growing plaque: a reaction-diffusion model. Biophys J. 1992 Jun;61(6):1540–1549. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...