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Antimicrob Agents Chemother. 1992 September; 36(9): 1859–1863. | PMCID: PMC192200 |
Liquid chromatographic determination of amoxicillin concentrations in bovine plasma by using a tandem solid-phase extraction method. H J Nelis, J Vandenbranden, A De Kruif, and A P De Leenheer Laboratoria voor Medische Biochemie en voor Klinische Analyse, Rijksuniversiteit Gent, Belgium. Abstract We report a means of determining amoxicillin in bovine plasma by liquid chromatography with UV detection at 235 nm. Purification and concentration of extracts were accomplished by a tandem solid-phase extraction procedure with two reversed-phase columns. Separation of amoxicillin from interferences was improved by the incorporation of a crown ether in the solvent systems used both for the solid-phase extraction and the final high-pressure liquid chromatography. Cefadroxil was added as an internal standard. The average recovery of amoxicillin from plasma (n = 23) was 78.2 +/- 3.0%, and the within-run and between-run coefficients of variation ranged from 1.8 to 7.0%. The detection limit was estimated at 0.1 microgram/ml. This method was used to determine amoxicillin in bovine plasma after intramuscular administration of the drug. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (864K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References. These references are in PubMed. This may not be the complete list of references from this article. - Carlqvist J, Westerlund D. Determination of amoxicillin in body fluids by reversed-phase liquid chromatography coupled with a post-column derivatization procedure. J Chromatogr. 1979 Nov 11;164(3):373–381. [PubMed]
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