• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
J Virol. Feb 1997; 71(2): 1713–1717.
PMCID: PMC191237

Construction of a genome-length cDNA clone for human astrovirus serotype 1 and synthesis of infectious RNA transcripts.

Abstract

We have constructed a genome-length cDNA clone for human astrovirus serotype 1. When a human colon cancer-derived cell line, CaCo-2, is transfected with RNA transcribed in vitro from this cDNA clone, infectious virus is produced at titers close to those observed after infection with intact astrovirus. A rodent cell line, BHK, which is largely refractory to astrovirus infection, was found to support efficient growth of the virus if transfected with viral RNA. The high transfection efficiency seen in the BHK cells allows studies of the viral replication in the transfected cells and thus should prove useful for the characterization of noninfectious astroviral mutants.

Full Text

The Full Text of this article is available as a PDF (173K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Boyer JC, Haenni AL. Infectious transcripts and cDNA clones of RNA viruses. Virology. 1994 Feb;198(2):415–426. [PubMed]
  • Burroughs JN, Brown F. Presence of a covalently linked protein on calicivirus RNA. J Gen Virol. 1978 Nov;41(2):443–446. [PubMed]
  • Cunningham TP, Montelaro RC, Rushlow KE. Lentivirus envelope sequences and proviral genomes are stabilized in Escherichia coli when cloned in low-copy-number plasmid vectors. Gene. 1993 Feb 14;124(1):93–98. [PubMed]
  • Dormitzer PR, Ho DY, Mackow ER, Mocarski ES, Greenberg HB. Neutralizing epitopes on herpes simplex virus-1-expressed rotavirus VP7 are dependent on coexpression of other rotavirus proteins. Virology. 1992 Mar;187(1):18–32. [PubMed]
  • Gorbalenya AE, Donchenko AP, Blinov VM, Koonin EV. Cysteine proteases of positive strand RNA viruses and chymotrypsin-like serine proteases. A distinct protein superfamily with a common structural fold. FEBS Lett. 1989 Jan 30;243(2):103–114. [PubMed]
  • Herrmann JE, Nowak NA, Perron-Henry DM, Hudson RW, Cubitt WD, Blacklow NR. Diagnosis of astrovirus gastroenteritis by antigen detection with monoclonal antibodies. J Infect Dis. 1990 Feb;161(2):226–229. [PubMed]
  • Herrmann JE, Taylor DN, Echeverria P, Blacklow NR. Astroviruses as a cause of gastroenteritis in children. N Engl J Med. 1991 Jun 20;324(25):1757–1760. [PubMed]
  • Jiang B, Monroe SS, Koonin EV, Stine SE, Glass RI. RNA sequence of astrovirus: distinctive genomic organization and a putative retrovirus-like ribosomal frameshifting signal that directs the viral replicase synthesis. Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10539–10543. [PMC free article] [PubMed]
  • Kraehenbuhl JP, Neutra MR. Molecular and cellular basis of immune protection of mucosal surfaces. Physiol Rev. 1992 Oct;72(4):853–879. [PubMed]
  • Lee TW, Kurtz JB. Serial propagation of astrovirus in tissue culture with the aid of trypsin. J Gen Virol. 1981 Dec;57(Pt 2):421–424. [PubMed]
  • Lewis TL, Greenberg HB, Herrmann JE, Smith LS, Matsui SM. Analysis of astrovirus serotype 1 RNA, identification of the viral RNA-dependent RNA polymerase motif, and expression of a viral structural protein. J Virol. 1994 Jan;68(1):77–83. [PMC free article] [PubMed]
  • Lewis TL, Matsui SM. An astrovirus frameshift signal induces ribosomal frameshifting in vitro. Arch Virol. 1995;140(6):1127–1135. [PubMed]
  • Liljeström P, Lusa S, Huylebroeck D, Garoff H. In vitro mutagenesis of a full-length cDNA clone of Semliki Forest virus: the small 6,000-molecular-weight membrane protein modulates virus release. J Virol. 1991 Aug;65(8):4107–4113. [PMC free article] [PubMed]
  • Madeley CR, Cosgrove BP. Letter: Viruses in infantile gastroenteritis. Lancet. 1975 Jul 19;2(7925):124–124. [PubMed]
  • Marczinke B, Bloys AJ, Brown TD, Willcocks MM, Carter MJ, Brierley I. The human astrovirus RNA-dependent RNA polymerase coding region is expressed by ribosomal frameshifting. J Virol. 1994 Sep;68(9):5588–5595. [PMC free article] [PubMed]
  • Meyers G, Thiel HJ, Rümenapf T. Classical swine fever virus: recovery of infectious viruses from cDNA constructs and generation of recombinant cytopathogenic defective interfering particles. J Virol. 1996 Mar;70(3):1588–1595. [PMC free article] [PubMed]
  • Meyers G, Wirblich C, Thiel HJ. Rabbit hemorrhagic disease virus--molecular cloning and nucleotide sequencing of a calicivirus genome. Virology. 1991 Oct;184(2):664–676. [PubMed]
  • Monroe SS, Jiang B, Stine SE, Koopmans M, Glass RI. Subgenomic RNA sequence of human astrovirus supports classification of Astroviridae as a new family of RNA viruses. J Virol. 1993 Jun;67(6):3611–3614. [PMC free article] [PubMed]
  • Monroe SS, Stine SE, Gorelkin L, Herrmann JE, Blacklow NR, Glass RI. Temporal synthesis of proteins and RNAs during human astrovirus infection of cultured cells. J Virol. 1991 Feb;65(2):641–648. [PMC free article] [PubMed]
  • Moormann RJ, van Gennip HG, Miedema GK, Hulst MM, van Rijn PA. Infectious RNA transcribed from an engineered full-length cDNA template of the genome of a pestivirus. J Virol. 1996 Feb;70(2):763–770. [PMC free article] [PubMed]
  • Palombo EA, Bishop RF. Annual incidence, serotype distribution, and genetic diversity of human astrovirus isolates from hospitalized children in Melbourne, Australia. J Clin Microbiol. 1996 Jul;34(7):1750–1753. [PMC free article] [PubMed]
  • Rice CM, Grakoui A, Galler R, Chambers TJ. Transcription of infectious yellow fever RNA from full-length cDNA templates produced by in vitro ligation. New Biol. 1989 Dec;1(3):285–296. [PubMed]
  • Sanchez-Fauquier A, Carrascosa AL, Carrascosa JL, Otero A, Glass RI, Lopez JA, San Martin C, Melero JA. Characterization of a human astrovirus serotype 2 structural protein (VP26) that contains an epitope involved in virus neutralization. Virology. 1994 Jun;201(2):312–320. [PubMed]
  • Schlesinger S. Alphaviruses--vectors for the expression of heterologous genes. Trends Biotechnol. 1993 Jan;11(1):18–22. [PubMed]
  • Sosnovtsev S, Green KY. RNA transcripts derived from a cloned full-length copy of the feline calicivirus genome do not require VpG for infectivity. Virology. 1995 Jul 10;210(2):383–390. [PubMed]
  • Wang CY, Dominguez G, Frey TK. Construction of rubella virus genome-length cDNA clones and synthesis of infectious RNA transcripts. J Virol. 1994 Jun;68(6):3550–3557. [PMC free article] [PubMed]
  • Willcocks MM, Brown TD, Madeley CR, Carter MJ. The complete sequence of a human astrovirus. J Gen Virol. 1994 Jul;75(Pt 7):1785–1788. [PubMed]
  • Willcocks MM, Carter MJ, Laidler FR, Madeley CR. Growth and characterisation of human faecal astrovirus in a continuous cell line. Arch Virol. 1990;113(1-2):73–81. [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...