• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
J Virol. Aug 1996; 70(8): 5634–5637.
PMCID: PMC190524

Transmissible gastroenteritis coronavirus, but not the related porcine respiratory coronavirus, has a sialic acid (N-glycolylneuraminic acid) binding activity.

Abstract

The hemagglutinating activity of transmissible gastroenteritis virus (TGEV), an enteric porcine coronavirus, was analyzed and found to be dependent on the presence of alpha-2,3-linked sialic acid on the erythrocyte surface. N-Glycolylneuraminic acid was recognized more efficiently by TGEV than was N-acetylneuraminic acid. For an efficient hemagglutination reaction the virions had to be treated with sialidase. This result suggests that the sialic acid binding site is blocked by virus-associated competitive inhibitors. Porcine respiratory coronavirus (PRCV), which is serologically related to TGEV but not enteropathogenic, was found to be unable to agglutinate erythrocytes. Incubation with sialidase did not induce a hemagglutinating activity of PRCV, indicating that the lack of this activity is an intrinsic property of the virus and not due to the presence of competitive inhibitors. Only monoclonal antibodies to an antigenic site that is absent from the S protein of PRCV were able to prevent TGEV from agglutinating erythrocytes. The epitope recognized by these antibodies is located within a stretch of 224 amino acids that is missing in the S protein of PRCV. Our results indicate that the sialic acid binding activity is also located in that portion of the S protein. The presence of a hemagglutinating activity in TGEV and its absence in PRCV open the possibility that the sialic acid binding activity contributes to the enterotropism of TGEV.

Full Text

The Full Text of this article is available as a PDF (187K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bernard S, Laude H. Site-specific alteration of transmissible gastroenteritis virus spike protein results in markedly reduced pathogenicity. J Gen Virol. 1995 Sep;76(Pt 9):2235–2241. [PubMed]
  • Bingham RW, Madge MH, Tyrrell DA. Haemagglutination by avian infectious bronchitis virus-a coronavirus. J Gen Virol. 1975 Sep;28(3):381–390. [PubMed]
  • Callebaut P, Correa I, Pensaert M, Jiménez G, Enjuanes L. Antigenic differentiation between transmissible gastroenteritis virus of swine and a related porcine respiratory coronavirus. J Gen Virol. 1988 Jul;69(Pt 7):1725–1730. [PubMed]
  • Correa I, Jiménez G, Suñ C, Bullido MJ, Enjuanes L. Antigenic structure of the E2 glycoprotein from transmissible gastroenteritis coronavirus. Virus Res. 1988 Apr;10(1):77–93. [PubMed]
  • Cox E, Pensaert MB, Callebaut P, van Deun K. Intestinal replication of a porcine respiratory coronavirus closely related antigenically to the enteric transmissible gastroenteritis virus. Vet Microbiol. 1990 Jun;23(1-4):237–243. [PubMed]
  • Delmas B, Gelfi J, Laude H. Antigenic structure of transmissible gastroenteritis virus. II. Domains in the peplomer glycoprotein. J Gen Virol. 1986 Jul;67(Pt 7):1405–1418. [PubMed]
  • Delmas B, Gelfi J, L'Haridon R, Vogel LK, Sjöström H, Norén O, Laude H. Aminopeptidase N is a major receptor for the entero-pathogenic coronavirus TGEV. Nature. 1992 Jun 4;357(6377):417–420. [PubMed]
  • Delmas B, Gelfi J, Sjöström H, Noren O, Laude H. Further characterization of aminopeptidase-N as a receptor for coronaviruses. Adv Exp Med Biol. 1993;342:293–298. [PubMed]
  • Gebauer F, Posthumus WP, Correa I, Suñ C, Smerdou C, Sánchez CM, Lenstra JA, Meloen RH, Enjuanes L. Residues involved in the antigenic sites of transmissible gastroenteritis coronavirus S glycoprotein. Virology. 1991 Jul;183(1):225–238. [PubMed]
  • Lepers A, Shaw L, Cacan R, Schauer R, Montreuil J, Verbert A. Transport of CMP-N-glycoloylneuraminic acid into mouse liver Golgi vesicles. FEBS Lett. 1989 Jul 3;250(2):245–250. [PubMed]
  • Morrison LA, Fields BN. Parallel mechanisms in neuropathogenesis of enteric virus infections. J Virol. 1991 Jun;65(6):2767–2772. [PMC free article] [PubMed]
  • Noda M, Koide F, Asagi M, Inaba Y. Physicochemical properties of transmissible gastroenteritis virus hemagglutinin. Arch Virol. 1988;99(3-4):163–172. [PubMed]
  • Noda M, Yamashita H, Koide F, Kadoi K, Omori T, Asagi M, Inaba Y. Hemagglutination with transmissible gastroenteritis virus. Arch Virol. 1987;96(1-2):109–115. [PubMed]
  • Pensaert M, Callebaut P, Vergote J. Isolation of a porcine respiratory, non-enteric coronavirus related to transmissible gastroenteritis. Vet Q. 1986 Jul;8(3):257–261. [PubMed]
  • Rasschaert D, Duarte M, Laude H. Porcine respiratory coronavirus differs from transmissible gastroenteritis virus by a few genomic deletions. J Gen Virol. 1990 Nov;71(Pt 11):2599–2607. [PubMed]
  • Sánchez CM, Jiménez G, Laviada MD, Correa I, Suñ C, Bullido M j, Gebauer F, Smerdou C, Callebaut P, Escribano JM, et al. Antigenic homology among coronaviruses related to transmissible gastroenteritis virus. Virology. 1990 Feb;174(2):410–417. [PubMed]
  • Sánchez CM, Gebauer F, Suñ C, Mendez A, Dopazo J, Enjuanes L. Genetic evolution and tropism of transmissible gastroenteritis coronaviruses. Virology. 1992 Sep;190(1):92–105. [PubMed]
  • Schultze B, Cavanagh D, Herrler G. Neuraminidase treatment of avian infectious bronchitis coronavirus reveals a hemagglutinating activity that is dependent on sialic acid-containing receptors on erythrocytes. Virology. 1992 Aug;189(2):792–794. [PubMed]
  • Schultze B, Gross HJ, Brossmer R, Herrler G. The S protein of bovine coronavirus is a hemagglutinin recognizing 9-O-acetylated sialic acid as a receptor determinant. J Virol. 1991 Nov;65(11):6232–6237. [PMC free article] [PubMed]
  • Schultze B, Gross HJ, Brossmer R, Klenk HD, Herrler G. Hemagglutinating encephalomyelitis virus attaches to N-acetyl-9-O-acetylneuraminic acid-containing receptors on erythrocytes: comparison with bovine coronavirus and influenza C virus. Virus Res. 1990 Jun;16(2):185–194. [PubMed]
  • Schultze B, Herrler G. Bovine coronavirus uses N-acetyl-9-O-acetylneuraminic acid as a receptor determinant to initiate the infection of cultured cells. J Gen Virol. 1992 Apr;73(Pt 4):901–906. [PubMed]
  • Schultze B, Wahn K, Klenk HD, Herrler G. Isolated HE-protein from hemagglutinating encephalomyelitis virus and bovine coronavirus has receptor-destroying and receptor-binding activity. Virology. 1991 Jan;180(1):221–228. [PubMed]
  • Suñ C, Jiménez G, Correa I, Bullido MJ, Gebauer F, Smerdou C, Enjuanes L. Mechanisms of transmissible gastroenteritis coronavirus neutralization. Virology. 1990 Aug;177(2):559–569. [PubMed]
  • Vlasak R, Luytjes W, Spaan W, Palese P. Human and bovine coronaviruses recognize sialic acid-containing receptors similar to those of influenza C viruses. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4526–4529. [PMC free article] [PubMed]
  • Wesley RD, Woods RD, Cheung AK. Genetic analysis of porcine respiratory coronavirus, an attenuated variant of transmissible gastroenteritis virus. J Virol. 1991 Jun;65(6):3369–3373. [PMC free article] [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...