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Fig. 2

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Depletion of CD14+ CD16+ monocytes by glucocorticoid (GC) therapy. Whole blood from patients before and after 5 days of daily treatment with 500 mg methylprednisolone was stained with directly fluorochrome-conjugated monoclonal antibodies (PE–anti-CD14 and FITC–anti-CD16 or FITC–anti-CD33) and 5000 cells per sample were analysed in the monocyte scatter gate by FACS. (a,b) Patient 3. (c,d) Patient 8. Absolute count of CD14+ CD16+ monocytes in patient 3 decreased from 52 cells/μl to eight cells/μl. CD14+ CD33+ monocytes in patient 8 decreased from 23 cells/μl to 0.3 cells/μl.

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