• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
J Virol. Dec 1995; 69(12): 7430–7436.
PMCID: PMC189680

High-titer packaging cells producing recombinant retroviruses resistant to human serum.

Abstract

Novel retroviral protein expression constructs were designed to retain minimal retroviral sequences and to express dominant selectable markers by reinitiation of translation after expression of the viral genes. HT1080 cells were selected as producer cells for their ability to release high-titer viruses that are resistant to inactivation by human serum. Two HT1080-based packaging cell lines which produce Moloney murine leukemia virus cores with envelope glycoproteins of either amphotropic murine leukemia virus (FLYA13 line) or cat endogenous virus RD114 (FLYRD18 line) are described. Direct comparison with previous retroviral packaging systems indicated that 100-fold-higher titers of helper-free recombinant viruses were released by the FLYA13 and FLYRD18 lines.

Full Text

The Full Text of this article is available as a PDF (209K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Battini JL, Heard JM, Danos O. Receptor choice determinants in the envelope glycoproteins of amphotropic, xenotropic, and polytropic murine leukemia viruses. J Virol. 1992 Mar;66(3):1468–1475. [PMC free article] [PubMed]
  • Benveniste RE, Todaro GJ. Homology between type-C viruses of various species as determined by molecular hybridization. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3316–3320. [PMC free article] [PubMed]
  • Bestwick RK, Kozak SL, Kabat D. Overcoming interference to retroviral superinfection results in amplified expression and transmission of cloned genes. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5404–5408. [PMC free article] [PubMed]
  • Cosset FL, Girod A, Flamant F, Drynda A, Ronfort C, Valsesia S, Molina RM, Faure C, Nigon VM, Verdier G. Use of helper cells with two host ranges to generate high-titer retroviral vectors. Virology. 1993 Mar;193(1):385–395. [PubMed]
  • Cosset FL, Legras C, Chebloune Y, Savatier P, Thoraval P, Thomas JL, Samarut J, Nigon VM, Verdier G. A new avian leukosis virus-based packaging cell line that uses two separate transcomplementing helper genomes. J Virol. 1990 Mar;64(3):1070–1078. [PMC free article] [PubMed]
  • Cosset FL, Legras C, Thomas JL, Molina RM, Chebloune Y, Faure C, Nigon VM, Verdier G. Improvement of avian leukosis virus (ALV)-based retrovirus vectors by using different cis-acting sequences from ALVs. J Virol. 1991 Jun;65(6):3388–3394. [PMC free article] [PubMed]
  • Danos O, Mulligan RC. Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges. Proc Natl Acad Sci U S A. 1988 Sep;85(17):6460–6464. [PMC free article] [PubMed]
  • Gatignol A, Durand H, Tiraby G. Bleomycin resistance conferred by a drug-binding protein. FEBS Lett. 1988 Mar 28;230(1-2):171–175. [PubMed]
  • Girod A, Cosset FL, Verdier G, Ronfort C. Analysis of ALV-based packaging cell lines for production of contaminant defective viruses. Virology. 1995 Jun 1;209(2):671–675. [PubMed]
  • Heard JM, Danos O. An amino-terminal fragment of the Friend murine leukemia virus envelope glycoprotein binds the ecotropic receptor. J Virol. 1991 Aug;65(8):4026–4032. [PMC free article] [PubMed]
  • Hu WS, Temin HM. Retroviral recombination and reverse transcription. Science. 1990 Nov 30;250(4985):1227–1233. [PubMed]
  • Izumi M, Miyazawa H, Kamakura T, Yamaguchi I, Endo T, Hanaoka F. Blasticidin S-resistance gene (bsr): a novel selectable marker for mammalian cells. Exp Cell Res. 1991 Dec;197(2):229–233. [PubMed]
  • Kavanaugh MP, Miller DG, Zhang W, Law W, Kozak SL, Kabat D, Miller AD. Cell-surface receptors for gibbon ape leukemia virus and amphotropic murine retrovirus are inducible sodium-dependent phosphate symporters. Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7071–7075. [PMC free article] [PubMed]
  • Kozak M. Effects of intercistronic length on the efficiency of reinitiation by eucaryotic ribosomes. Mol Cell Biol. 1987 Oct;7(10):3438–3445. [PMC free article] [PubMed]
  • Kozak SL, Kabat D. Ping-pong amplification of a retroviral vector achieves high-level gene expression: human growth hormone production. J Virol. 1990 Jul;64(7):3500–3508. [PMC free article] [PubMed]
  • Markowitz D, Goff S, Bank A. Construction and use of a safe and efficient amphotropic packaging cell line. Virology. 1988 Dec;167(2):400–406. [PubMed]
  • Miller AD. Retrovirus packaging cells. Hum Gene Ther. 1990 Spring;1(1):5–14. [PubMed]
  • Miller AD. Human gene therapy comes of age. Nature. 1992 Jun 11;357(6378):455–460. [PubMed]
  • Miller AD, Garcia JV, von Suhr N, Lynch CM, Wilson C, Eiden MV. Construction and properties of retrovirus packaging cells based on gibbon ape leukemia virus. J Virol. 1991 May;65(5):2220–2224. [PMC free article] [PubMed]
  • Miller DG, Miller AD. A family of retroviruses that utilize related phosphate transporters for cell entry. J Virol. 1994 Dec;68(12):8270–8276. [PMC free article] [PubMed]
  • Muenchau DD, Freeman SM, Cornetta K, Zwiebel JA, Anderson WF. Analysis of retroviral packaging lines for generation of replication-competent virus. Virology. 1990 May;176(1):262–265. [PubMed]
  • Ott D, Friedrich R, Rein A. Sequence analysis of amphotropic and 10A1 murine leukemia viruses: close relationship to mink cell focus-inducing viruses. J Virol. 1990 Feb;64(2):757–766. [PMC free article] [PubMed]
  • Perryman S, Nishio J, Chesebro B. Complete nucleotide sequence of Friend murine leukemia virus, strain FB29. Nucleic Acids Res. 1991 Dec 25;19(24):6950–6950. [PMC free article] [PubMed]
  • Reeves RH, O'Brien SJ. Molecular genetic characterization of the RD-114 gene family of endogenous feline retroviral sequences. J Virol. 1984 Oct;52(1):164–171. [PMC free article] [PubMed]
  • Ronfort C, Girod A, Cosset FL, Legras C, Nigon VM, Chebloune Y, Verdier G. Defective retroviral endogenous RNA is efficiently transmitted by infectious particles produced on an avian retroviral vector packaging cell line. Virology. 1995 Feb 20;207(1):271–275. [PubMed]
  • Russell SJ, Hawkins RE, Winter G. Retroviral vectors displaying functional antibody fragments. Nucleic Acids Res. 1993 Mar 11;21(5):1081–1085. [PMC free article] [PubMed]
  • Scadden DT, Fuller B, Cunningham JM. Human cells infected with retrovirus vectors acquire an endogenous murine provirus. J Virol. 1990 Jan;64(1):424–427. [PMC free article] [PubMed]
  • Scarpa M, Cournoyer D, Muzny DM, Moore KA, Belmont JW, Caskey CT. Characterization of recombinant helper retroviruses from Moloney-based vectors in ecotropic and amphotropic packaging cell lines. Virology. 1991 Feb;180(2):849–852. [PubMed]
  • Shih A, Misra R, Rush MG. Detection of multiple, novel reverse transcriptase coding sequences in human nucleic acids: relation to primate retroviruses. J Virol. 1989 Jan;63(1):64–75. [PMC free article] [PubMed]
  • Shinnick TM, Lerner RA, Sutcliffe JG. Nucleotide sequence of Moloney murine leukaemia virus. Nature. 1981 Oct 15;293(5833):543–548. [PubMed]
  • Sommerfelt MA, Weiss RA. Receptor interference groups of 20 retroviruses plating on human cells. Virology. 1990 May;176(1):58–69. [PubMed]
  • Sommerfelt MA, Williams BP, McKnight A, Goodfellow PN, Weiss RA. Localization of the receptor gene for type D simian retroviruses on human chromosome 19. J Virol. 1990 Dec;64(12):6214–6220. [PMC free article] [PubMed]
  • Tailor CS, Takeuchi Y, O'Hara B, Johann SV, Weiss RA, Collins MK. Mutation of amino acids within the gibbon ape leukemia virus (GALV) receptor differentially affects feline leukemia virus subgroup B, simian sarcoma-associated virus, and GALV infections. J Virol. 1993 Nov;67(11):6737–6741. [PMC free article] [PubMed]
  • Takeuchi Y, Cosset FL, Lachmann PJ, Okada H, Weiss RA, Collins MK. Type C retrovirus inactivation by human complement is determined by both the viral genome and the producer cell. J Virol. 1994 Dec;68(12):8001–8007. [PMC free article] [PubMed]
  • Torrent C, Bordet T, Darlix JL. Analytical study of rat retrotransposon VL30 RNA dimerization in vitro and packaging in murine leukemia virus. J Mol Biol. 1994 Jul 29;240(5):434–444. [PubMed]
  • Vanin EF, Kaloss M, Broscius C, Nienhuis AW. Characterization of replication-competent retroviruses from nonhuman primates with virus-induced T-cell lymphomas and observations regarding the mechanism of oncogenesis. J Virol. 1994 Jul;68(7):4241–4250. [PMC free article] [PubMed]
  • Zhang J, Temin HM. Retrovirus recombination depends on the length of sequence identity and is not error prone. J Virol. 1994 Apr;68(4):2409–2414. [PMC free article] [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...