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J Virol. Sep 1995; 69(9): 5455–5460.
PMCID: PMC189393

Positionally independent and exchangeable late budding functions of the Rous sarcoma virus and human immunodeficiency virus Gag proteins.

Abstract

The Gag proteins of Rous sarcoma virus and human immunodeficiency virus (HIV) each contain a function involved in a late step in budding, defects in which result in the accumulation of these molecules at the plasma membrane. In the Rous sarcoma virus Gag protein (Pr76gag), this assembly domain is associated with a PPPY motif, which is located at an internal position between the MA and CA sequences. This motif is not contained anywhere within the HIV Gag protein (Pr55gag), and the MA sequence is linked directly to CA. Instead, a late assembly function of HIV has been associated with the p6 sequence situated at the C terminus of Gag. Here we demonstrate the remarkable finding that the late assembly domains from these two unrelated Gag proteins are exchangeable between retroviruses and can function in a positionally independent manner.

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Selected References

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  • Becker J, Craig EA. Heat-shock proteins as molecular chaperones. Eur J Biochem. 1994 Jan 15;219(1-2):11–23. [PubMed]
  • Bennett RP, Nelle TD, Wills JW. Functional chimeras of the Rous sarcoma virus and human immunodeficiency virus gag proteins. J Virol. 1993 Nov;67(11):6487–6498. [PMC free article] [PubMed]
  • Bennett RP, Rhee S, Craven RC, Hunter E, Wills JW. Amino acids encoded downstream of gag are not required by Rous sarcoma virus protease during gag-mediated assembly. J Virol. 1991 Jan;65(1):272–280. [PMC free article] [PubMed]
  • Blond-Elguindi S, Cwirla SE, Dower WJ, Lipshutz RJ, Sprang SR, Sambrook JF, Gething MJ. Affinity panning of a library of peptides displayed on bacteriophages reveals the binding specificity of BiP. Cell. 1993 Nov 19;75(4):717–728. [PubMed]
  • Craven RC, Bennett RP, Wills JW. Role of the avian retroviral protease in the activation of reverse transcriptase during virion assembly. J Virol. 1991 Nov;65(11):6205–6217. [PMC free article] [PubMed]
  • Craven RC, Leure-duPree AE, Erdie CR, Wilson CB, Wills JW. Necessity of the spacer peptide between CA and NC in the Rous sarcoma virus gag protein. J Virol. 1993 Oct;67(10):6246–6252. [PMC free article] [PubMed]
  • Craven RC, Leure-duPree AE, Weldon RA, Jr, Wills JW. Genetic analysis of the major homology region of the Rous sarcoma virus Gag protein. J Virol. 1995 Jul;69(7):4213–4227. [PMC free article] [PubMed]
  • Göttlinger HG, Dorfman T, Sodroski JG, Haseltine WA. Effect of mutations affecting the p6 gag protein on human immunodeficiency virus particle release. Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3195–3199. [PMC free article] [PubMed]
  • Hartl FU, Hlodan R, Langer T. Molecular chaperones in protein folding: the art of avoiding sticky situations. Trends Biochem Sci. 1994 Jan;19(1):20–25. [PubMed]
  • Henderson LE, Bowers MA, Sowder RC, 2nd, Serabyn SA, Johnson DG, Bess JW, Jr, Arthur LO, Bryant DK, Fenselau C. Gag proteins of the highly replicative MN strain of human immunodeficiency virus type 1: posttranslational modifications, proteolytic processings, and complete amino acid sequences. J Virol. 1992 Apr;66(4):1856–1865. [PMC free article] [PubMed]
  • Henderson LE, Sowder RC, Smythers GW, Oroszlan S. Chemical and immunological characterizations of equine infectious anemia virus gag-encoded proteins. J Virol. 1987 Apr;61(4):1116–1124. [PMC free article] [PubMed]
  • Hoshikawa N, Kojima A, Yasuda A, Takayashiki E, Masuko S, Chiba J, Sata T, Kurata T. Role of the gag and pol genes of human immunodeficiency virus in the morphogenesis and maturation of retrovirus-like particles expressed by recombinant vaccinia virus: an ultrastructural study. J Gen Virol. 1991 Oct;72(Pt 10):2509–2517. [PubMed]
  • Jowett JB, Hockley DJ, Nermut MV, Jones IM. Distinct signals in human immunodeficiency virus type 1 Pr55 necessary for RNA binding and particle formation. J Gen Virol. 1992 Dec;73(Pt 12):3079–3086. [PubMed]
  • Montelaro RC, Lohrey N, Parekh B, Blakeney EW, Issel CJ. Isolation and comparative biochemical properties of the major internal polypeptides of equine infectious anemia virus. J Virol. 1982 Jun;42(3):1029–1038. [PMC free article] [PubMed]
  • Paxton W, Connor RI, Landau NR. Incorporation of Vpr into human immunodeficiency virus type 1 virions: requirement for the p6 region of gag and mutational analysis. J Virol. 1993 Dec;67(12):7229–7237. [PMC free article] [PubMed]
  • Payne SL, Rausch J, Rushlow K, Montelaro RC, Issel C, Flaherty M, Perry S, Sellon D, Fuller F. Characterization of infectious molecular clones of equine infectious anaemia virus. J Gen Virol. 1994 Feb;75(Pt 2):425–429. [PubMed]
  • Pepinsky RB, Mattaliano RJ, Vogt VM. Structure and processing of the p2 region of avian sarcoma and leukemia virus gag precursor polyproteins. J Virol. 1986 Apr;58(1):50–58. [PMC free article] [PubMed]
  • Royer M, Cerutti M, Gay B, Hong SS, Devauchelle G, Boulanger P. Functional domains of HIV-1 gag-polyprotein expressed in baculovirus-infected cells. Virology. 1991 Sep;184(1):417–422. [PubMed]
  • Stys D, Blaha I, Strop P. Structural and functional studies in vitro on the p6 protein from the HIV-1 gag open reading frame. Biochim Biophys Acta. 1993 Sep 8;1182(2):157–161. [PubMed]
  • Vogt VM, Eisenman R, Diggelmann H. Generation of avian myeloblastosis virus structural proteins by proteolytic cleavage of a precursor polypeptide. J Mol Biol. 1975 Aug 15;96(3):471–493. [PubMed]
  • Weldon RA, Jr, Erdie CR, Oliver MG, Wills JW. Incorporation of chimeric gag protein into retroviral particles. J Virol. 1990 Sep;64(9):4169–4179. [PMC free article] [PubMed]
  • Weldon RA, Jr, Wills JW. Characterization of a small (25-kilodalton) derivative of the Rous sarcoma virus Gag protein competent for particle release. J Virol. 1993 Sep;67(9):5550–5561. [PMC free article] [PubMed]
  • Wills JW, Cameron CE, Wilson CB, Xiang Y, Bennett RP, Leis J. An assembly domain of the Rous sarcoma virus Gag protein required late in budding. J Virol. 1994 Oct;68(10):6605–6618. [PMC free article] [PubMed]
  • Wills JW, Craven RC. Form, function, and use of retroviral gag proteins. AIDS. 1991 Jun;5(6):639–654. [PubMed]
  • Wills JW, Craven RC, Achacoso JA. Creation and expression of myristylated forms of Rous sarcoma virus gag protein in mammalian cells. J Virol. 1989 Oct;63(10):4331–4343. [PMC free article] [PubMed]
  • Wills JW, Craven RC, Weldon RA, Jr, Nelle TD, Erdie CR. Suppression of retroviral MA deletions by the amino-terminal membrane-binding domain of p60src. J Virol. 1991 Jul;65(7):3804–3812. [PMC free article] [PubMed]

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