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Am J Pathol. 1993 Jul; 143(1): 211–220.
PMCID: PMC1886958

Nuclear A-type lamins are differentially expressed in human lung cancer subtypes.


Nuclear A-type and B-type lamin expression was investigated in the major human lung cancer subtypes: small cell lung cancer (SCLC), squamous cell carcinomas, and adenocarcinomas (both non-SCLC). Twenty-two human lung cancer cell lines and 46 fresh frozen human lung cancer specimens were examined. Expression of B-type lamins was found in all the different cell lines. A-type lamins were expressed in all non-SCLC cell lines but were absent or only weakly expressed in 14 out of 16 SCLC cell lines. The immunocytochemical results were confirmed by immunoblotting and Northern blot analyses. In sections of SCLCs and non-SCLCs, B-type lamins were found to be expressed in all tumors. However, in some non-SCLCs, particularly in adenocarcinomas, a considerable proportion of the tumor cells were negative for B-type lamins. A-type lamin expression in SCLCs was weakly positive or negative in 14 out of 15 cases. In contrast, all non-SCLCs displayed A-type lamins, but in several of these samples, both cytoplasmic and nuclear staining was observed.

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  • McKeon FD, Kirschner MW, Caput D. Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins. Nature. 1986 Feb 6;319(6053):463–468. [PubMed]
  • Fisher DZ, Chaudhary N, Blobel G. cDNA sequencing of nuclear lamins A and C reveals primary and secondary structural homology to intermediate filament proteins. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6450–6454. [PMC free article] [PubMed]
  • Höger TH, Zatloukal K, Waizenegger I, Krohne G. Characterization of a second highly conserved B-type lamin present in cells previously thought to contain only a single B-type lamin. Chromosoma. 1990 Oct;99(6):379–390. [PubMed]
  • Lebel S, Lampron C, Royal A, Raymond Y. Lamins A and C appear during retinoic acid-induced differentiation of mouse embryonal carcinoma cells. J Cell Biol. 1987 Sep;105(3):1099–1104. [PMC free article] [PubMed]
  • Stewart C, Burke B. Teratocarcinoma stem cells and early mouse embryos contain only a single major lamin polypeptide closely resembling lamin B. Cell. 1987 Nov 6;51(3):383–392. [PubMed]
  • Röber RA, Sauter H, Weber K, Osborn M. Cells of the cellular immune and hemopoietic system of the mouse lack lamins A/C: distinction versus other somatic cells. J Cell Sci. 1990 Apr;95(Pt 4):587–598. [PubMed]
  • Guilly MN, Kolb JP, Gosti F, Godeau F, Courvalin JC. Lamins A and C are not expressed at early stages of human lymphocyte differentiation. Exp Cell Res. 1990 Jul;189(1):145–147. [PubMed]
  • Kaufmann SH, Mabry M, Jasti R, Shaper JH. Differential expression of nuclear envelope lamins A and C in human lung cancer cell lines. Cancer Res. 1991 Jan 15;51(2):581–586. [PubMed]
  • Bepler G, Jaques G, Neumann K, Aumüller G, Gropp C, Havemann K. Establishment, growth properties, and morphological characteristics of permanent human small cell lung cancer cell lines. J Cancer Res Clin Oncol. 1987;113(1):31–40. [PubMed]
  • Bepler G, Jaques G, Havemann K, Koehler A, Johnson BE, Gazdar AF. Characterization of two cell lines with distinct phenotypes established from a patient with small cell lung cancer. Cancer Res. 1987 Apr 1;47(7):1883–1891. [PubMed]
  • Bepler G, Koehler A, Kiefer P, Havemann K, Beisenherz K, Jaques G, Gropp C, Haeder M. Characterization of the state of differentiation of six newly established human non-small-cell lung cancer cell lines. Differentiation. 1988;37(2):158–171. [PubMed]
  • Broers JL, Rot MK, Oostendorp T, Bepler G, De Leij L, Carney DN, Vooijs GP, Ramaekers FC. Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines. J Cell Sci. 1988 Sep;91(Pt 1):91–108. [PubMed]
  • de Leij L, Postmus PE, Buys CH, Elema JD, Ramaekers F, Poppema S, Brouwer M, van der Veen AY, Mesander G, The TH. Characterization of three new variant type cell lines derived from small cell carcinoma of the lung. Cancer Res. 1985 Dec;45(12 Pt 1):6024–6033. [PubMed]
  • Carney DN, Gazdar AF, Bepler G, Guccion JG, Marangos PJ, Moody TW, Zweig MH, Minna JD. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 1985 Jun;45(6):2913–2923. [PubMed]
  • Gazdar AF, Bunn PA, Jr, Minna JD, Baylin SB. Origin of human small cell lung cancer. Science. 1985 Aug 16;229(4714):679–680. [PubMed]
  • Broers JL, Rot MK, Oostendorp T, Huysmans A, Wagenaar SS, Wiersma-van Tilburg AJ, Vooijs GP, Ramaekers FC. Immunocytochemical detection of human lung cancer heterogeneity using antibodies to epithelial, neuronal, and neuroendocrine antigens. Cancer Res. 1987 Jun 15;47(12):3225–3234. [PubMed]
  • Broers JL, Ramaekers FC, Rot MK, Oostendorp T, Huysmans A, van Muijen GN, Wagenaar SS, Vooijs GP. Cytokeratins in different types of human lung cancer as monitored by chain-specific monoclonal antibodies. Cancer Res. 1988 Jun 1;48(11):3221–3229. [PubMed]
  • Broers JL, Carney DN, Klein Rot M, Schaart G, Lane EB, Vooijs GP, Ramaekers FC. Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies. J Cell Sci. 1986 Jul;83:37–60. [PubMed]
  • Lehner CF, Stick R, Eppenberger HM, Nigg EA. Differential expression of nuclear lamin proteins during chicken development. J Cell Biol. 1987 Jul;105(1):577–587. [PMC free article] [PubMed]
  • Burke B, Tooze J, Warren G. A monoclonal antibody which recognises each of the nuclear lamin polypeptides in mammalian cells. EMBO J. 1983;2(3):361–367. [PMC free article] [PubMed]
  • Zatloukal K, Denk H, Spurej G, Hutter H. Modulation of protein composition of nuclear lamina. Reduction of lamins B1 and B2 in livers of griseofulvin-treated mice. Lab Invest. 1992 May;66(5):589–597. [PubMed]
  • Auffray C, Rougeon F. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem. 1980 Jun;107(2):303–314. [PubMed]
  • Lehrach H, Diamond D, Wozney JM, Boedtker H. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry. 1977 Oct 18;16(21):4743–4751. [PubMed]
  • Thomas PS. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. [PMC free article] [PubMed]
  • Church GM, Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. [PMC free article] [PubMed]
  • Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. [PubMed]
  • Fort P, Marty L, Piechaczyk M, el Sabrouty S, Dani C, Jeanteur P, Blanchard JM. Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family. Nucleic Acids Res. 1985 Mar 11;13(5):1431–1442. [PMC free article] [PubMed]
  • Krohne G, Benavente R. The nuclear lamins. A multigene family of proteins in evolution and differentiation. Exp Cell Res. 1986 Jan;162(1):1–10. [PubMed]
  • Gerace L, Burke B. Functional organization of the nuclear envelope. Annu Rev Cell Biol. 1988;4:335–374. [PubMed]
  • Nigg EA. Assembly-disassembly of the nuclear lamina. Curr Opin Cell Biol. 1992 Feb;4(1):105–109. [PubMed]
  • Stick R, Hausen P. Changes in the nuclear lamina composition during early development of Xenopus laevis. Cell. 1985 May;41(1):191–200. [PubMed]
  • Benavente R, Krohne G, Franke WW. Cell type-specific expression of nuclear lamina proteins during development of Xenopus laevis. Cell. 1985 May;41(1):177–190. [PubMed]
  • Houliston E, Guilly MN, Courvalin JC, Maro B. Expression of nuclear lamins during mouse preimplantation development. Development. 1988 Feb;102(2):271–278. [PubMed]
  • Röber RA, Weber K, Osborn M. Differential timing of nuclear lamin A/C expression in the various organs of the mouse embryo and the young animal: a developmental study. Development. 1989 Feb;105(2):365–378. [PubMed]
  • Franke WW, Schmid E, Winter S, Osborn M, Weber K. Widespread occurrence of intermediate-sized filaments of the vimentin-type in cultured cells from diverse vertebrates. Exp Cell Res. 1979 Oct 1;123(1):25–46. [PubMed]
  • Ben-Ze'ev A, Robinson GS, Bucher NL, Farmer SR. Cell-cell and cell-matrix interactions differentially regulate the expression of hepatic and cytoskeletal genes in primary cultures of rat hepatocytes. Proc Natl Acad Sci U S A. 1988 Apr;85(7):2161–2165. [PMC free article] [PubMed]
  • Gerace L, Comeau C, Benson M. Organization and modulation of nuclear lamina structure. J Cell Sci Suppl. 1984;1:137–160. [PubMed]
  • Loewinger L, McKeon F. Mutations in the nuclear lamin proteins resulting in their aberrant assembly in the cytoplasm. EMBO J. 1988 Aug;7(8):2301–2309. [PMC free article] [PubMed]
  • Moir RD, Donaldson AD, Stewart M. Expression in Escherichia coli of human lamins A and C: influence of head and tail domains on assembly properties and paracrystal formation. J Cell Sci. 1991 Jun;99(Pt 2):363–372. [PubMed]
  • Worman HJ, Lazaridis I, Georgatos SD. Nuclear lamina heterogeneity in mammalian cells. Differential expression of the major lamins and variations in lamin B phosphorylation. J Biol Chem. 1988 Aug 25;263(24):12135–12141. [PubMed]
  • Raats JM, Henderik JB, Verdijk M, van Oort FL, Gerards WL, Ramaekers FC, Bloemendal H. Assembly of carboxy-terminally deleted desmin in vimentin-free cells. Eur J Cell Biol. 1991 Oct;56(1):84–103. [PubMed]
  • Raats JM, Pieper FR, Vree Egberts WT, Verrijp KN, Ramaekers FC, Bloemendal H. Assembly of amino-terminally deleted desmin in vimentin-free cells. J Cell Biol. 1990 Nov;111(5 Pt 1):1971–1985. [PMC free article] [PubMed]
  • Heitlinger E, Peter M, Lustig A, Villiger W, Nigg EA, Aebi U. The role of the head and tail domain in lamin structure and assembly: analysis of bacterially expressed chicken lamin A and truncated B2 lamins. J Struct Biol. 1992 Jan-Feb;108(1):74–89. [PubMed]
  • Sternberger LA, Sternberger NH. Monoclonal antibodies distinguish phosphorylated and nonphosphorylated forms of neurofilaments in situ. Proc Natl Acad Sci U S A. 1983 Oct;80(19):6126–6130. [PMC free article] [PubMed]
  • Peter M, Nakagawa J, Dorée M, Labbé JC, Nigg EA. In vitro disassembly of the nuclear lamina and M phase-specific phosphorylation of lamins by cdc2 kinase. Cell. 1990 May 18;61(4):591–602. [PubMed]
  • Georgatos SD, Weber K, Geisler N, Blobel G. Binding of two desmin derivatives to the plasma membrane and the nuclear envelope of avian erythrocytes: evidence for a conserved site-specificity in intermediate filament-membrane interactions. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6780–6784. [PMC free article] [PubMed]
  • Georgatos SD, Blobel G. Lamin B constitutes an intermediate filament attachment site at the nuclear envelope. J Cell Biol. 1987 Jul;105(1):117–125. [PMC free article] [PubMed]
  • Lebkowski JS, Laemmli UK. Non-histone proteins and long-range organization of HeLa interphase DNA. J Mol Biol. 1982 Apr 5;156(2):325–344. [PubMed]
  • Benavente R, Krohne G. Involvement of nuclear lamins in postmitotic reorganization of chromatin as demonstrated by microinjection of lamin antibodies. J Cell Biol. 1986 Nov;103(5):1847–1854. [PMC free article] [PubMed]
  • Stuurman N, Meijne AM, van der Pol AJ, de Jong L, van Driel R, van Renswoude J. The nuclear matrix from cells of different origin. Evidence for a common set of matrix proteins. J Biol Chem. 1990 Apr 5;265(10):5460–5465. [PubMed]
  • Collard JF, Senécal JL, Raymond Y. Redistribution of nuclear lamin A is an early event associated with differentiation of human promyelocytic leukemia HL-60 cells. J Cell Sci. 1992 Mar;101(Pt 3):657–670. [PubMed]
  • Collard JF, Raymond Y. Phorbol esters induce transient changes in the accessibility of the carboxy-terminal domain of nuclear lamin A. Exp Cell Res. 1992 Jul;201(1):174–183. [PubMed]

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