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Antimicrob Agents Chemother. Sep 1993; 37(9): 1856–1859.
PMCID: PMC188082

Age and therapeutic outcome of experimental Pseudomonas aeruginosa keratitis treated with ciprofloxacin, prednisolone, and flurbiprofen.

Abstract

This study was conducted to determine whether the age of the host influences the pathogenesis and therapeutic outcome of drug-treated Pseudomonas aeruginosa keratitis. Young (3- to 5-month-old) and old (1.5- to 3-year-old) rabbits were intrastromally infected with P. aeruginosa ATCC 27853. Sixteen hours later, rabbits in both age subpopulations were divided into three groups and treated topically as follows: group 1, phosphate-buffered saline; group 2, 0.3% ciprofloxacin; and group 3, 0.3% ciprofloxacin, 1.0% prednisolone, and 0.03% flurbiprofen. Drops were given every 15 min for 1 h and then every 30 min for 9 h. At 27 h postinfection, ocular pathology was graded with a slit lamp examination (SLE) scoring system. Aqueous humor was collected for ciprofloxacin quantitation, and corneas were harvested for bacterial enumeration and estimation of polymorphonuclear leukocytes. Young rabbits had more severe inflammation and pathology than old rabbits. At 27 h postinfection, SLE scores and polymorphonuclear leukocyte numbers were significantly higher for young rabbits than old rabbits (P < 0.02), regardless of treatment. Prednisolone and flurbiprofen significantly reduced SLE scores in both age groups (P < 0.03) without affecting the antimicrobial efficacy of ciprofloxacin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Fox RA. Atypical presentation of geriatric infections. Geriatrics. 1988 May;43(5):58–68. [PubMed]
  • Gray LD, Kreger AS. Rabbit corneal damage produced by Pseudomonas aeruginosa infection. Infect Immun. 1975 Aug;12(2):419–432. [PMC free article] [PubMed]
  • Gritz DC, Lee TY, Kwitko S, McDonnell PJ. Topical anti-inflammatory agents in an animal model of microbial keratitis. Arch Ophthalmol. 1990 Jul;108(7):1001–1005. [PubMed]
  • Hazlett LD, Kreindler FB, Berk RS, Barrett R. Aging alters the phagocytic capability of inflammatory cells induced into cornea. Curr Eye Res. 1990 Feb;9(2):129–138. [PubMed]
  • Hobden JA, O'Callaghan RJ, Hill JM, Hagenah M, Insler MS, Reidy JJ. Ciprofloxacin and prednisolone therapy for experimental Pseudomonas keratitis. Curr Eye Res. 1992 Mar;11(3):259–265. [PubMed]
  • Hobden JA, Reidy JJ, O'Callaghan RJ, Insler MS, Hill JM. Ciprofloxacin iontophoresis for aminoglycoside-resistant pseudomonal keratitis. Invest Ophthalmol Vis Sci. 1990 Oct;31(10):1940–1944. [PubMed]
  • Johnson MK, Hobden JA, Hagenah M, O'Callaghan RJ, Hill JM, Chen S. The role of pneumolysin in ocular infections with Streptococcus pneumoniae. Curr Eye Res. 1990 Nov;9(11):1107–1114. [PubMed]
  • Matsumoto K, Shams NB, Hanninen LA, Kenyon KR. Cleavage and activation of corneal matrix metalloproteases by Pseudomonas aeruginosa proteases. Invest Ophthalmol Vis Sci. 1993 May;34(6):1945–1953. [PubMed]
  • Nanda M, Pflugfelder SC, Holland S. Fulminant pseudomonal keratitis and scleritis in human immunodeficiency virus-infected patients. Arch Ophthalmol. 1991 Apr;109(4):503–505. [PubMed]
  • Ohadi C, Litwin KL, Moreira H, Kwitko S, Gauderman WJ, Gritz DC, Gwon A, Jones R, McDonnell PJ. Anti-inflammatory therapy and outcome in a guinea pig model of Pseudomonas keratitis. Cornea. 1992 Sep;11(5):398–403. [PubMed]
  • Rootman DS, Hobden JA, Jantzen JA, Gonzalez JR, O'Callaghan RJ, Hill JM. Iontophoresis of tobramycin for the treatment of experimental Pseudomonas keratitis in the rabbit. Arch Ophthalmol. 1988 Feb;106(2):262–265. [PubMed]
  • Simons RJ, Reynolds HY. Altered immune status in the elderly. Semin Respir Infect. 1990 Dec;5(4):251–259. [PubMed]
  • Solana R, Villanueva JL, Peña J, De la Fuente M. Cell mediated immunity in ageing. Comp Biochem Physiol A Comp Physiol. 1991;99(1-2):1–4. [PubMed]
  • Steuhl KP, Döring G, Henni A, Thiel HJ, Botzenhart K. Relevance of host-derived and bacterial factors in Pseudomonas aeruginosa corneal infections. Invest Ophthalmol Vis Sci. 1987 Sep;28(9):1559–1568. [PubMed]
  • Williams RN, Paterson CA, Eakins KE, Bhattacherjee P. Quantification of ocular inflammation: evaluation of polymorphonuclear leucocyte infiltration by measuring myeloperoxidase activity. Curr Eye Res. 1982;2(7):465–470. [PubMed]

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